Structural heterogeneity

The structural and density heterogeneity of the liquid 3Al2O3.2SiO2 (l-3Al2O3.2SiO2) system was studied using Molecular Dynamics (MD) and Monte Carlo (MC) simulations. The results showed that the structural phase transition occurred at an oxygen packing factor of approximately 0.58.

8p vimitsuki 30-03-2025 6 0   Download

The paper is aimed at providing approaches to the analysis of buildings alternative to simplified methods usually adopted in professional practice, which do typically involve drastic simplifications of the fabric.

10p tohitohi 19-05-2020 8 1   Download

We report here the direct evidence for the formation of alternative DNA structures in a plasmid DNA, termed pTIR10, containing a 0.23-kb pyrimidine/purine-biased (Pyr/Pur) stretch isolated from the rat genome. Long Pyr/Pur sequences are abundant in eukaryotic genomes, and they may modulate the biological activity of genes and genomes via formation of various types of triplex-related structures. The plasmid DNA in sodium acetate buffer (pH 4.35) was deposited on APS-modified mica, and after drying it was imaged with an atomic force microscope in air. ...

5p system191 01-06-2013 34 3   Download

The heterogeneous nuclear ribonucleoprotein (hnRNP) type I, a modulator of alternative splicing, localizes in the nucleoplasm of mammalian cells and in a discrete perinucleolar structure. HnRNP I contains a novel type of bipartite nuclear localization signal (NLS) at the N-terminus of the protein that we have previously named nuclear determinant localization type I (NLD-I). Recently, a neural counterpart of hnRNP I has been identified that contains a putative NLS with two strings of basic amino acids separated by a spacer of 30 residues....

8p system191 01-06-2013 26 3   Download

Structural elucidation of the lipopolysaccharide (LPS) from three nontypeable Haemophilus influenzaeclinical isolates, 1209, 1207 and 1233 was achieved using NMR spectro-scopy and ESI-MS on O-deacylated LPS and core oligo-saccharide (OS) material as well as ESI-MS n on permethylated dephosphorylated OS. It was found that the organisms expressed a tremendous heterogeneous glyco-form mixture resulting from the variable length of the OS chains attached to the common structural element of H.

15p tumor12 20-04-2013 37 2   Download

Therehas longbeenconjectureover thedegree towhichthere may be structural and functional heterogeneity in the tetra-mericserineproteasetryptase(EC 3.4.21.59), amajor mediator of allergic inflammation. We have applied 2D gel electrophoresis to analyze the extent, nature, and variability of thisheterogeneity inlysatesofmast cells isolatedfromlung and skin, and in preparations of purified tryptase. Gels were silver stained, or the proteins transferred to nitrocellulose blots and probed with either tryptase-specific monoclonal antibodies or various lectins. ...

14p tumor12 20-04-2013 42 3   Download

Structural analysis of the lipopolysaccharide (LPS) from nontypeableHaemophilus influenzaestrain 981 has been achieved using NMRspectroscopy and ESI-MS on O-deacylatedLPSand core oligosaccharide (OS)material as well as by ESI-MS n on permethylated dephosphorylated OS. A heterogeneous glycoform population was identified, resulting from the variable length of the OS branches attached to the glucose residue in the common structural element of H. influenzae LPS, L-a-D-Hepp-(1fi2)-[PEtnfi6]-L-a-D-Hepp-(1fi3)-[b-D-Glcp-(1fi4)]-L-a-D-Hepp-(1fi5)-[PPEtnfi4]-a-Kdop-(2fi6)-Lipid A....

13p fptmusic 16-04-2013 48 3   Download

We used a PCR-based method to determine the genomic DNA sequences encoding phospholipases A2(PLA2s) from the venoms ofVipera aspis aspis(V. a. aspis),Vipera aspis zinnikeri (V. a. zinnikeri), Vipera berus berus(V. b. berus) and a neurotoxicV. a. aspissnake (neurotoxicV. a. aspis) from a population responsible for unusual neurotoxic envenomations in south-east France. We sequenced five groups of genes, each corresponding to a different PLA2.

10p fptmusic 16-04-2013 44 4   Download

bR, N-like and O-like intermediate states of [ 15 N]methionine-labelled wild type and D85N⁄T170C bacteriorhodopsin were accumulated in native membranes by controlling the pH of the preparations. 15 N cross polariza-tion and magic angle sample spinning (CPMAS) NMR spectroscopy allowed resolution of seven out of nine resonances in the bR-state. It was possible to assign some of the observed resonances by using 13 C⁄ 15 N rota

0p awards 06-04-2013 28 3   Download

Fifty-nine amino acid sequences belonging to family 57 (GH-57) of the glycoside hydrolases were collected using the CAZy server, Pfamdatabase andBLASTtools. Owing to the sequence heterogeneity of the GH-57 members, sequence alignments were performed using mainly manual methods. Likewise, five conserved regions were identified, which are postulated to be GH-57 consensus motifs.

10p dell39 03-04-2013 35 3   Download

The catalytic module ofHypocrea jecorina(previously Trichoderma reesei) Cel7B was homologously expressed by transformation of strain QM9414. Post-translational modi-fications in purified Cel7B preparations were analysed by enzymatic digestions, high performance chromatography, mass spectrometry and site-directed mutagenesis. Of the five potential sites found in the wild-type enzyme, only Asn56 and Asn182 were found to be N-glycosylated.

11p dell39 03-04-2013 45 5   Download

Acharan sulfate, a recently discovered glycosaminoglycan isolated from Achatina fulica, has a major disaccharide repeating unit of fi4)-2-acetyl,2-deoxy-a-D-glucopyra-nose(1fi4)-2-sulfo-a-L-idopyranosyluronic acid (1fi,mak-ing it structurally related to both heparin and heparan sulfate. It has been suggested that this glycosaminoglycan is polydisperse, with an average molecular mass of 29 kDa andknownminor disaccharide sequence variants containing unsulfated iduronic acid.

10p dell39 03-04-2013 41 4   Download

Patterns of protein interactions are organized around complex heterogene-ous networks. Their architecture has been suggested to be of relevance in understanding the interactome and its functional organization, which per-vades cellular robustness. Transcription factors are particularly relevant in this context, given their central role in gene regulation.

12p dell39 27-03-2013 39 3   Download

Protein glycation by methylglyoxal is a nonenzymatic post-translational modification whereby arginine and lysine side chains form a chemically heterogeneous group of advanced glycation end-products. Methylglyoxal-derived advanced glycation end-products are involved in pathologies such as diabetes and neurodegenerative diseases of the amyloid type.

15p inspiron33 23-03-2013 53 4   Download

Parkinson’s disease (PD) is a heterogeneous movement disorder character-ized by progressive degeneration of dopamine neurons in substantia nigra. We have previously presented genetic evidence for the possible involvement of alcohol and aldehyde dehydrogenases (ADH; ALDH) by identifying genetic variants in ADH1C and ADH4 that associate with PD.

12p galaxyss3 21-03-2013 37 4   Download

The structure of human telomeric DNA is controversial; it depends upon the sequence contexts and the methodologies used to determine it. The solution structure in the presence of K + is particularly interesting, but the structure is yet to be elucidated, due to possible conformational heterogen-eity.

12p galaxyss3 19-03-2013 37 3   Download

The hnRNP K homology (KH) domain was first identified in the protein human heterogeneous nuclear ribonucleoprotein K (hnRNP K) 14 years ago. Since then, KH domains have been identified as nucleic acid recognition motifs in proteins that perform a wide range of cellular functions.

15p media19 06-03-2013 36 3   Download

The interaction of multiple HIV-1 regulator of virion (Rev) proteins with the viral RNA target, the Rev response element (RRE), is critical for nuclear export of incompletely spliced and unspliced viral RNA, and for the onset of the late phase in the viral replication cycle. The heterogeneity of the Rev–RRE complex has made it difficult to study using conventional structural methods.

10p vinaphone15 25-02-2013 27 4   Download

Hyaluronate lyases are a class of endoglycosaminidase enzymes with a high level of complexity and heterogeneity. The main function of the Streptococ-cus pyogenesbacteriophage protein hyaluronate lyase, HylP2, is to degrade hyaluronan into unsaturated disaccharide units. HylP2 was cloned, over-expressed and purified to homogeneity.

11p viettel02 22-02-2013 42 3   Download

A great deal must still be learnt on the structural features of amyloid assemblies, particularly prefibrillar aggregates, and the relationship of the latter with amyloid cytotoxicity. Presently, it is recognized that the popula-tion of unstable, heterogeneous amyloid oligomers and protofibrils is mainly responsible for amyloid cytotoxicity.

12p viettel02 19-02-2013 38 4   Download