Targeted deletion

Long-read sequencing is increasingly used to uncover structural variants in the human genome, both functionally neutral and deleterious. Structural variants occur more frequently in regions with a high homology or repetitive segments, and one rearrangement may predispose to additional events.

17p vicwell 29-02-2024 3 1   Download

Head spot is one of the phenotypes identified in the KFRS4/Kyo rat strain. Although previous linkage analysis suggested that Ednrb, which is frequently involved in coat color variations in various animals, could be the gene responsible for this phenotype, no mutations have been identified in its coding region.

8p vinarcissa 21-03-2023 3 1   Download

In higher vertebrates the notochord is a transient rod-like structure in the midline of the embryo. In mouse embryos beginning at embryonic stage E8.5 the notochord arises as a distinct anatomical entity. Its anterior end is close to Radtke’s pouch from which it extends posteriorly to the tip of the tail.

17p vinarcissa 21-03-2023 1 1   Download

The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and deletion of chromosomal segments. However, whether an entire chromosome could be eliminated by this technology is still unknown.

18p vialfrednobel 29-01-2022 14 0   Download

The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task.

3p viaristotle 29-01-2022 8 0   Download

Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field, has critical limitations for detecting and quantifying structural variants such as large deletions (greater than approximately 100 base pairs), inversions, and translocations.

10p vibeauty 23-10-2021 13 0   Download

The recent advances in agricultural biotechnology and genetic engineering have brought numerous benefits to the food and agricultural sector by improving the essential characteristics of plant agronomic traits. Targeted genome editing using sequence specific nucleases (SSNs) provides a general method for inducing targeted deletions, insertions and precise sequence changes in a broad range of organisms and cell types.

15p chauchaungayxua11 23-03-2021 12 2   Download

We further confirm the specificity of toxin-conjugated anti-CD137 mAb showing that only T cells that expressed CD137 underwent apoptosis by staining with toxin-FITC-mAb conjugates. We also found that toxinconjugated anti-CD137 mAb was able to delete allore-active T cells in vitro. Our result indicates that CD137 targeted delivery of toxin is a good strategy to delete antigen-specific T cells.

8p tamynhan8 04-11-2020 7 1   Download

The EGFR 3′ untranslated region (UTR) harbors a polyadenine repeat which is polymorphic (A13/A14) and undergoes somatic deletions in microsatellite instability (MSI) colorectal cancer (CRC). These mutations could be oncogenic in colorectal tissue since they were shown to result into increased EGFR mRNA stability in CRC cell lines.

8p vijennie2711 29-09-2020 19 2   Download

MicroRNAs (miRNAs) are frequently dysregulated in human cancers and can act as either potent oncogenes or tumor suppressor genes. In the present study, we intend to prove that the gene PTEN (phosphatase and tensin homolog deleted on chromosome ten) is a target gene of miR-205 and to investigate the suppressive effects on PTEN transcriptional activity by enhancing miR-205 expression in endometrial cancer Ishikawa cells.

8p virose2711 22-09-2020 13 1   Download

Conditional deletion of the tumour suppressor gene Apc within the murine intestine results in acute Wnt signalling activation. The associated over-expression of a myriad of Wnt signalling target genes yields phenotypic alterations that encompass many of the hallmarks of neoplasia.

9p vialabama2711 21-09-2020 6 0   Download

In invasive malignancies, Dll4/Notch signaling inhibition enhances non-functional vessel proliferation and limits tumor growth by reducing its blood perfusion. Methods: To assess the effects of targeted Dll4 allelic deletion in the incipient stages of tumor pathogenesis, we chemically induced skin papillomas in wild-type and Dll4+/− littermates, and compared tumor growth, their histological features, vascularization and the expression of angiogenesis-related molecules.

9p viamsterdam 18-09-2020 15 1   Download

Genetic alterations in pediatric primary brain tumors can be used as diagnostic and prognostic markers and are the basis for the development of new target therapies that, ideally, would be associated with lower mortality and morbidity.

10p viputrajaya2711 22-06-2020 5 0   Download

Practical approaches for multigene transformation and gene stacking are extremely important for engineering complex traits and adding new traits in transgenic crops. Trait deployment by gene stacking would greatly simplify downstream plant breeding and trait introgression into cultivars.

12p viuchiha2711 21-04-2020 7 0   Download

Next-generation sequencing (NGS) is a high throughput sequencing technology, which has revolutionized both basic and clinical research of the human genetic disorders. This technology is also called massively parallel sequencing (MPS) due to its ability to generate a huge amount of output data in a cost- and time-effective manner. NGS is widely utilized for different sequencing applications such as targeted sequencing (a group of candidate genes), exome sequencing (all coding regions), and whole genome sequencing (the entire human genome).

14p caygaocaolon1 13-11-2019 26 1   Download

Non - small cell lung cancer (NSCLC) is the largest subgroup of lung cancers, responsible for about 80% of all lung cancers. Targeted cancer therapy has opened a new window into treatment of non - small cell lung cancer. This therapy is very effective for patients who have EGFR (Epidermal growth factor receptor) activating mutations, primarily located in the tyrosine kinase domain in the form of a base - pair deletion at exon 19 (LREA deletions, accounting for about 54%) or a point mutation at exon 21 (L858R, accounting for about 43%).

9p quenchua1 04-11-2019 11 0   Download

Trim bodies View a topic Trim Body Split Body Trimmed Sheet Trim and Extend Untrim Divide Face Delete Edge 1. Trim Body Use Trim Body to trim one or more target bodies using a face or plane. You can specify the portion of the body to retain and the portion to discard. The target bodies take the shape of the trimming geometry.

33p allspackht 13-06-2013 52 11   Download

ACCELERATION Launching Pad Accelerators Macros Summary FOCUS Kill Distractions Search Trumps Navigation Find Hard Targets Use Rooted Views Use Sticky Attributes Use Project-Based Shortcuts Multiply Your Monitors Segregate Your Workspace with Virtual Desktops Summary AUTOMATION Don’t Reinvent Wheels Cache Stuff Locally Automate Your Interaction with Web Sites Interact with RSS Feeds Subvert Ant for Non-Build Tasks Subvert Rake for Common Tasks Subvert Selenium to Walk Web Pages Use Bash to Harvest Exception Counts Replace Batch Files with Windows Power Shell Use Mac OS X Automator to Delete ...

224p trac2_123 16-04-2013 59 3   Download

Oracle SQL Developer is a graphical tool that lets you browse, create, edit, and delete (drop) database objects, edit and debug PL/SQL code, run SQL statements and scripts, manipulate and export data, and create and view reports. With SQL Developer, you can connect to any target Oracle Database schema using standard Oracle Database authentication. Once connected, you can perform operations on objects in the database.

308p nhacsihuytuan 13-04-2013 55 3   Download

A small deletion in the second intron of humanNDUFV2 (IVS2+5_+8delGTAA) has been shown to cause hyper-trophic cardiomyopathy and encephalomyopathy [Be´nit, P., Beugnot, R., Chretien, D., Giurgea, I., de Lonlay-Debeney, P., Issartel, J.P., Kerscher, S., Rustin, P., Ro¨tig, A. & Munnich, A. (2003)Human Mutat.21, 582–586]. Skipping of exon 2 results in a partial deletion of the mitochondrial targeting sequence of the precursor for the 24 kDa subunit of respiratory chain complex I.

8p dell39 03-04-2013 42 2   Download