Time expressions

This review explores recent ML advancements in assessing corrosion in RC structures. Various algorithms, such as Artificial Neural Networks (ANNs), Gene Expression Programming (GEP), Extreme Gradient Boosting (XGBoost), Support Vector Machine (SVM) and Ensemble Learning, have shown potential in estimating corrosion processes, predicting material properties, and evaluating structural durability.

7p vibenya 31-12-2024 0 0   Download

Consider a system with a single degree of freedom and assume that the equation expressing its dynamic equilibrium is a second order ordinary differential equation (ODE) in the generalized coordinate x.

164p hiruscar 19-10-2010 87 8   Download

Bài giảng "Vi xử lý - Chương 3.8: System và program developments of 8051" giới thiệu tới người đọc các nội dung: Assembly Language Programming, Assembly Language Program Format, Assemble-Time Expression Evaluation, Assembler Directives, Assembler State Controls, Storage Initialization/Reservation,... Mời các bạn cùng tham khảo nội dung chi tiết.

49p nhansinhaoanh_03 22-10-2015 88 6   Download

Tham khảo “Đề cương ôn tập giữa học kì 1 môn Tiếng Anh lớp 7 năm 2022-2023 - Trường THCS Dương Nội” để bổ sung kiến thức, nâng cao tư duy và rèn luyện kỹ năng giải đề chuẩn bị thật tốt cho kì thi học kì sắp tới các em nhé! Chúc các em ôn tập kiểm tra đạt kết quả cao!

11p bongbay01 05-05-2023 9 3   Download

Lesson plan number 9 have content the lesson belongs to Unit 8. A date to remember of New Headway elementary book. It introduces the basic knowledge about the usage of past simple 2, time expressions; vocabulary about relationships, spelling and silent letters; reading about three inventions; speaking about famous inventions; writing about a friend.

5p mailan1977 15-08-2016 90 6   Download

Mitochondrial functional and structural impairment and generation of oxidative stress have been implicated in aging, various diseases and chemotherapies. This study analyzed azidothymidine (AZT)-caused failures in mitochondrial functions, in redox regulation and activation of the HIV-1 gene expression. We monitored intracellular concentrations of ATP and glutathione (GSH) as the indicators of energy production and redox conditions, respectively, during the time-course experiments with U937 and MOLT4 human lymphoid cells in the presence of AZT (0.05 mgÆmL)1) or H2O2 (0.01 mM) for 15–25 days.

7p system191 01-06-2013 43 3   Download

Cloning and over-expression of human glucose 6-phosphate dehydrogenase (Glc6P dehydrogenase) has for the first time allowed a detailed kinetic study of a preparation that is genetically homogeneous and in which all the protein molecules are of identical age. The steady-state kinetics of the recombinant enzyme, studied by fluorimetric initial-rate measurements, gave converging linear Lineweaver–Burk plots as expected for a ternary-complex mechanism.

8p research12 01-06-2013 63 4   Download

Acomputational approach is used to analyse temporal gene expression in the context of metabolic regulation. It is based on the assumption that cells developed optimal adaptation strategies to changing environmental conditions. Time-dependent enzyme profiles are calculatedwhichoptimize the function of a metabolic pathway under the constraint of limited total enzymeamount.For linearmodel pathways it is shown thatwave-like enzyme profiles are optimal for a rapid substrate turnover.

8p tumor12 22-04-2013 49 3   Download

Human D2Long(D2L)andD2Short(D2S) dopamine receptor isoforms were modified at their N-terminus by the addition of a human immunodeficiency virus (HIV) or a FLAG epitope tag. The receptors were then expressed inSpodop-tera frugiperda 9(Sf9)cells using the baculovirus system, and their oligomerization was investigated by means of co-immunoprecipitation and time-resolved fluorescence resonance energy transfer (FRET).

11p tumor12 20-04-2013 39 2   Download

cAMP receptor protein (CRP), allosterically activated by cAMP, regulates the expression of several genes inEscheri-chia coli. As binding of cAMP leads to undefined conform-ational changes in CRP, we performed a steady-state and time-resolved fluorescence study to showhow the binding of the ligand influences the structure and dynamics of the protein. We used CRP mutants containing a single trypto-phan residue at position 85 or 13, and fluorescently labeled with 1,5-I-AEDANS attached toCys178.

11p tumor12 20-04-2013 31 3   Download

An efficient bacterial expression system of cyanobacterium Synechocystissp. PCC 6803 heme oxygenase gene, ho-1, has been constructed, using a synthetic gene. A soluble protein was expressedat high levels and was highly purified, for the first time. The protein binds equimolar free hemin to catabolize the boundhemin to ferric-bili-verdin IXa in the presence of oxygen andreducing equivalents, showing the heme oxygenase activity. During the reaction, verdoheme intermediate is formed with the evolution of carbon monoxide. ...

12p tumor12 20-04-2013 32 4   Download

The expression of the major protein kinase C substrate MARCKS (myristoylatedalanine-richCkinase substrate) is controlled by the stability of its mRNA. While the MARCKS mRNA is long living in quiescent fibroblasts (t1/2¼14 h), its half-life time is drastically reduced (t1/2¼2 h) in cells treated with phorbol esters to activate protein kinase C (PKC) or treatedwith growth factors. In a first step to study the underlying mechanism we identified both acis-element on the MARCKS mRNA and the cor-responding trans-acting factors. ...

16p tumor12 20-04-2013 40 3   Download

TheST2gene, which is specifically induced by growth sti-mulation in fibroblasts, encodes interleukin-1 receptor-rela-tedproteins and iswidely expressed in hematopoietic, helper T, and various cancer cells. However, the physiological as well as pathological functions of the ST2 gene products are not yet fully understood. In this study, we analyzed the expression of theST2gene in human glioma cell lines and human brain tumor samples with real-time polymerase chain reactionmethod,

8p tumor12 20-04-2013 48 4   Download

Acetylcholinesterase is an enzyme whose best-known function is to hydro-lyze the neurotransmitter acetylcholine. Acetylcholinesterase is expressed in several noncholinergic tissues. Accordingly, we report for the first time the identification of acetylcholinesterase in human umbilical cord vein endo-thelial cells. Here we further performed an electrophoretic and biochemical characterization of this enzyme, using protein extracts obtained by solubili-zation of human endothelial cell membranes with Triton X-100. ...

11p fptmusic 12-04-2013 46 3   Download

In this study, we have characterized the influence of hypoxia on the expression of hydroxylases crucially involved in col-lagen fiber formation, such as prolyl-4-hydroxylases (Ph4) and procollagen lysyl-hydroxylases (PLOD). Using the rat vascular smooth muscle cell line A7r5, we found that an hypoxic atmosphere caused a characteristic time-dependent five- to 12-fold up-regulation of the mRNAs of the two P4h a-subunits [aI (P4ha1) andaII (P4ha2)] and of two lysyl-hydroxylases (PLOD1 and PLOD2).

8p fptmusic 12-04-2013 30 3   Download

For a long time, the haemerythrin family of proteins was considered to be restricted to only a few phyla of marine invertebrates. When analysing dif-ferential protein expression in the methane-oxidizing bacterium, Methylo-coccus capsulatus(Bath), grown at a high and low copper-to-biomass ratio, respectively, we identified a putative prokaryotic haemerythrin expressed in high-copper cultures.

0p awards 06-04-2013 30 1   Download

Thebform of human cAMP-dependent protein kinase inhibitor (human PKIb), a novel heat-stable protein, was isolated with high yield using a bacterial expression system. Assays of PKI activity demonstrated that purified PKIb inhibits the catalytic subunit of cAMP-dependent protein kinase. FTIR, Raman spectroscopy and CD experiments implied that human PKIbcontained only small amounts of a-helix andb-structures, but large amounts of random coil and turn structures, which may explain its high thermosta-bility.

6p dell39 03-04-2013 46 4   Download

A generic sample normalization method applicable in relative comparison of mRNAs quantified with real-time polymerase chain reaction (PCR) is proposed. The method was applied in samples obtained from tomato seeds after osmopriming and aging treatments and from untreated seeds at early imbibition stage, when seeds had not completed germination.

8p dell39 27-03-2013 51 2   Download

Control analysis of the glycolytic flux was carried out in two fast-growth tumor cell types of human and rodent origin (HeLa and AS-30D, respect-ively). Determination of the maximal velocity (Vmax) of the 10 glycolytic enzymes from hexokinase to lactate dehydrogenase revealed that hexokin-ase (153–306 times) and phosphfructokinase-1 (PFK-1) (22–56 times) had higher over-expression in rat AS-30D hepatoma cells than in normal freshly isolated rat hepatocytes.

14p inspiron33 26-03-2013 48 5   Download

The mechanism behind the growth-promoting effect of insulin is a subject of debate. Employing RT4 bladder cancer cells, we examined the cross-talk between insulin and the epidermal growth factor system. We found that insulin induced a time- and dose-dependent (25–1000 nmolÆL )1 insulin) increase in mRNA expression of three ligands from the epidermal growth factor system.

11p inspiron33 23-03-2013 41 3   Download