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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:12

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An efficient bacterial expression system of cyanobacterium Synechocystissp. PCC 6803 heme oxygenase gene, ho-1, has been constructed, using a synthetic gene. A soluble protein was expressedat high levels and was highly purified, for the first time. The protein binds equimolar free hemin to catabolize the boundhemin to ferric-bili-verdin IXa in the presence of oxygen andreducing equivalents, showing the heme oxygenase activity. During the reaction, verdoheme intermediate is formed with the evolution of carbon monoxide. ...

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Nội dung Text: Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme

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