Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme
33
lượt xem 4
download
lượt xem 4
download
Download
Vui lòng tải xuống để xem tài liệu đầy đủ
An efficient bacterial expression system of cyanobacterium Synechocystissp. PCC 6803 heme oxygenase gene, ho-1, has been constructed, using a synthetic gene. A soluble protein was expressedat high levels and was highly purified, for the first time. The protein binds equimolar free hemin to catabolize the boundhemin to ferric-bili-verdin IXa in the presence of oxygen andreducing equivalents, showing the heme oxygenase activity. During the reaction, verdoheme intermediate is formed with the evolution of carbon monoxide. ...
Chủ đề:
Bình luận(0) Đăng nhập để gửi bình luận!
CÓ THỂ BẠN MUỐN DOWNLOAD