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Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme
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Malate dehydrogenase (MDH) may be important in car-bohydrate and energymetabolism inmalarial parasites. The cDNA corresponding to the MDHgene, identified on chromosome 6 of thePlasmodium falciparumgenome, was amplified by RT-PCR, cloned and overexpressed in Escherichia coli. The recombinant PfMDH was purified to homogeneity and biochemically characterized as an NAD + (H)-specific MDH, which catalysed reversible inter-conversion of malate to oxaloacetate.
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