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Báo cáo khoa học: Analysis of the vacuolar luminal proteome of Saccharomyces cerevisiae
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Despite its large size and the numerous processes in which it is implicated, neither the identity nor the functions of the proteins targeted to the yeast vacuole have been defined comprehensively. In order to establish a method-ological platform and protein inventory to address this shortfall, we refined techniques for the purification of ‘proteomics-grade’ intact vacuoles.
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