intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:10

41
lượt xem
3
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

The HeLa cell terminal uridylyltransferase (TUTase) that specifically modifies the 3¢-end of mammalian U6 small nuclear RNA (snRNA) was characterized with respect to ionic dependence and substrate requirements. Optimal enzyme activity was obtained at moderate ionic strength (60 mMKCl) anddependedon thepresenceof 5 mMMgCl2. In vitrosynthesizedU6 snRNA without a 3¢-terminal UMP residue was not accepted as substrate.

Chủ đề:
Lưu

Nội dung Text: Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
3=>0