Báo cáo khoa học: Biosynthesis of riboflavin 6,7-Dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe
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A cDNA sequence fromSchizosaccharomyces pombewith similarity to 6,7-dimethyl-8-ribityllumazine synthase was expressed in a recombinant Escherichia colistrain. The recombinant protein is ahomopentamer of 17-kDa subunits with an apparent molecular mass of 87 kDa as determined by sedimentation equilibriumcentrifugation (it sediments at an apparent velocity of 5.0 S at 20°C). The protein has been crystallized in space group C2221 .
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