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Báo cáo khoa học: Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:8

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The nitrilase AtNIT1 fromArabidopsis thalianawas over-expressed inEscherichia coliwith an N-terminal His6tag and puri®ed by zinc chelate anity chromatography in a single step almost to homogeneity in a 68% yield with a speci®c activity of 34.1 Uámg )1 . The native enzyme (450 kDa) consists of 11±13 subunits (38 kDa). The temperature optimum was determined to be 35°C, and a pH optimum of 9 was found.

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Nội dung Text: Báo cáo khoa học: Characterization and synthetic applications of recombinant AtNIT1 from Arabidopsis thaliana

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