Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase
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TheLactococcus lactisNRRL B-1821 prolidase gene was cloned and over-expressed in Escherichia coli. Under suboptimum growth conditions, recombinant soluble and active prolidase was produced; in contrast, inclu-sion bodies were formed under conditions preferred for cell growth.
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