Báo cáo khoa học: Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea
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The basidiomyceteCoprinopsis cinereaproduces the glycoside hydrolase family 6 enzyme CcCel6C at low and constitutive levels. CcCel6C exhibits unusual cellobiohydrolase activity; it hydrolyses carboxymethyl cellulose, which is a poor substrate for typical cellobiohydrolases. Here, we deter-mined the crystal structures of CcCel6C unbound and in complex with p-nitrophenyl b-d-cellotrioside and cellobiose.
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