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Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:7

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We have developed a highly productive cell-free protein synthesis system from wheat germ, which is expected to become an important tool for postgenomic research. However, this system has not been optimized for the synthesis of disulfide-containing proteins. Thus, we sear-ched here for translation conditions under which a model protein, a single-chain antibody variable fragment (scFv), could be synthesized into its active form.

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Nội dung Text: Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ

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