Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus
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The role of DNase I hypersensitive sites (DHSs) in transcription of the B cell-specificIg-bgene and in maintenance of active chromatin state in the Ig-b locus were examined. A total of 10 DHSs were divided into four regions, and each region was deleted separately in chicken B lymphocyte-derived DT40 cells. Deletion of three DHSs located between theIg-bpro-moter and its upstreamNa channelgene, resulted in the absence of Ig-b mRNA. Three regions except the region in theNa channelgene were involved in the transcription of Ig-bgene. ...
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