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Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:9

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Mutations in the proteina-tropomyosin (Tm) can cause a disease known as familial hypertrophic cardiomyopathy. In order to understand how such mutations lead to protein dysfunction, three point mutations were introduced into cDNA encoding the human skeletal tropomyosin, and the recombinant Tms were produced at high levels in the yeast Pichia pastoris. Two mutations (A63V and K70T) were located in theN-terminal regionofTmandone (E180G)was located close to the calcium-dependent troponin T binding domain....

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Nội dung Text: Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin

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