Báo cáo khoa học: Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis
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Penicillin amidase fromAlcaligenes faecalisis a recently identifiedN-terminal nucleophilehydrolase,whichpossesses the highest specificity constant (kcat /Km)forthehydrolysis of benzylpenicillin compared with penicillin amidases from other sources. Similar to theEscherichia colipenicillin ami-dase, theA. faecalispenicillin amidase is maturatedin vivo from an inactive precursor into the catalytically active enzyme, containing one tightly bound Ca 2+ ion, via a complex post-translational autocatalytic processing with a multi-step excision of a small internal pro-peptide. ...
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