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Báo cáo khoa học: Kinetic mechanisms of glycine oxidase fromBacillus subtilis

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:9

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The kinetic properties of glycine oxidase fromBacillus sub-tiliswere investigated using glycine, sarcosine, andD-proline as substrate. The turnover numbers at saturating substrate andoxygen concentrationswere 4.0 s )1 ,4.2s )1 ,and3.5 s )1 , respectively, with glycine, sarcosine, andD-proline as sub-strate. Glycine oxidase was converted to a two-electron reduced form upon anaerobic reduction with the individual substrates and its reductive half-reaction was demonstrated tobe reversible.

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Nội dung Text: Báo cáo khoa học: Kinetic mechanisms of glycine oxidase fromBacillus subtilis

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