Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase
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To shed light on the mechanism of isotopic exchange of a-protons in amino acids catalyzed by pyridoxal phosphate (PLP)-dependent enzymes, we studied the kinetics of quinonoid intermediate formation for the reactions of tyrosine phenol-lyase with L-phenylalanine, L-methionine, and theira-deuterated analogues inD2O, andwe compared the results with the rates of the isotopic exchange under the same conditions.Wehave found that, in theL-phenylalanine reaction, the internal returnof thea-proton is operative, and allowing for its effect, the exchange rate is accounted for satisfactorily....
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