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Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein
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To determine the glycoforms of squid rhodopsin, N-glycans were released by glycoamidase A digestion, reductively aminated with 2-aminopyridine, and then subjected to 2D HPLC analysis [Takahashi, N., Nakagawa, H., Fujikawa, K.,Kawamura,Y.&Tomiya,N. (1995)Anal. Biochem.226, 139–146]. Themajor glycans of squid rhodopsinwere shown to possess the a1–3 and a1–6 difucosylated innermost GlcNAc residue found in glycoproteins produced by insects and helminths.
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