Báo cáo khoa học: On the mechanism of a-amylase Acarbose and cyclodextrin inhibition of barley amylase isozymes
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Two inhibitors, acarbose and cyclodextrins (CD), were used to investigate the active site structure and function of barleya-amylase isozymes, AMY1 and AMY2. The hydrolysis of DP 4900-amylose, reduced (r) DP18-malto-dextrin and maltoheptaose (catalysed by AMY1 and AMY2) was followed in the absence and in the presence of inhibitor. Without inhibitor, the highest activity was obtained with amylose,kcat /Kmdecreased 10 3 -fold using rDP18-maltodextrin and 10 5 to 10 6 -fold using maltohep-taose as substrate. ...
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