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Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli
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IbeA of Escherichia coliK1 was cloned, expressed and purified as a His6-tag fusion protein. The purified fusion protein inhibited E. coliK1 invasion of human brain microvascular endothelial cells and was heat-modifiable. The structural and functional aspects, along with equilibrium unfolding of IbeA, were studied in solution.
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