Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry
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Human alcohol dehydrogenase 3/glutathione-dependent formaldehyde dehydrogenase was shown to rapidly and irreversibly catalyse the reductive breakdown of S-nitroso-glutathione. The steady-state kinetics of S-nitrosogluta-thione reduction was studied for the wild-type and two mutated forms of human alcohol dehydrogenase 3, muta-tions that have previously been shown to affect the oxidative efficiency for the substrate S-hydroxymethylglutathione.
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