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Báo cáo khoa học: Structural basis for stereo-specific catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:13

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NAD + -dependent (R)-2-hydroxyglutarate dehydrogenase (HGDH) cata-lyses the reduction of 2-oxoglutarate to (R)-2-hydroxyglutarate and belongs to the D-2-hydroxyacid NAD + -dependent dehydrogenase (D-2-hydroxyacid dehydrogenase) protein family. Its crystal structure was determined by phase combination to 1.98 A˚ resolution. Structure–function relationships obtained by the comparison of HGDH with other members of theD-2-hydroxyacid dehydrogenase family give a chemically satisfying view of the substrate stereoselectivity and catalytic requirements for the hydride trans-fer reaction. A model for substrate recognition and turnover is discussed....

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Nội dung Text: Báo cáo khoa học: Structural basis for stereo-specific catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans

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