
Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies
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Schizophyllum commune a,a-trehalose phosphorylase utilizes a glycosyl-transferase-like catalytic mechanism to convert its disaccharide substrate into a-d-glucose 1-phosphate and a-d-glucose. Recruitment of phosphate by the free enzyme inducesa,a-trehalose binding recognition and promotes the catalytic steps.
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