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Báo cáo khoa học: Unfolding process of rusticyanin Evidence of protein aggregation

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:9

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The unfolding process of the Blue Copper Protein (BCP) rusticyanin (Rc) has been studied using a wide variety of biochemical techniques. Fluorescence and CD spectroscop-ies reveal that the copper ion plays an essential role in sta-bilizing the protein and that the oxidized form is more efficient than the reducedspecies in this respect. Theaddition of guanidinium chloride to Rc samples produces aggrega-tion of the protein. Gel filtration chromatography and glutaraldehyde cross-linking experiments confirm the for-mation of such aggregates. ...

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