Một số kết quả nghiên cứu về chất diệt khuẩn cho xử lý nước bơm ép

Hdi thdo khoa hoc "Ndng cao he sdthu hdi ddu md Bqch Hd"<br /> <br /> <br /> <br /> <br /> MOT S 6 K^T QUA NGHIEN ccru v^ CHAT DI$T KHUAN CHO X(J LY NLTOC BOM t?<br /> Cao Anh Dung, Ta Dinh Vinh, Tran Xuan Phuang<br /> Le Anh Dao, Le Minh San, Nguyen Lan Huang<br /> Tnnh Thanh San, Hoang Linh, Than Hoang Cudng<br /> Cong ty Dung dich khoan va Hoa pham Dau khi<br /> <br /> ABSTRACT<br /> Production of Hydrogen Sunfide in Sea-Water due to the Activitiy of Sulfat Reducing Bacteha (SRB) is<br /> potentially serious problem. Sea-Water contains all of the nutrients required to support SRB metabolism. This<br /> paper will present results from testing biocides, which producted by DMC controlling SRB. Results indicated th<br /> biocide C (Mixture of aldehydes C1-C5, amine and surfactant) was effective against SRB at lOOppm concentr<br /> whereas biocide A (Thazine), biocide B (condensation product of acrolein and formaldehyde) at the 200 ppm<br /> concentration. Tests have also shown that H2S production was halted almost immediately after one week of<br /> treatment.<br /> <br /> Viec sCr dung nude bien ed ham lugng ion S04^' cao va vi khuan khCr Sunfat (SRB) de bdm ep cd the gay<br /> ra nhieu thiet hai cho md. Su hoat ddng cua vi khuan SRB gay ra an mdn trong he thdng bam ep do sinh khi<br /> HjS va lam tac nghen dudng dng vi tao ra bun sat. Nguy hai han, viec hinh thanh HjS trong tang chira dau do vi<br /> khuan SRB khu ion SO/" lam ngudn dau va khi bi chua hoa.<br /> <br /> Theo nghien eCru eua eae nha vi sinh, vi khuan khCr Sunfat la tap hgp nhdm cac vi khuan dac biet cd<br /> trong mdi trudng nude thieu oxy cd ten la Desulforibno, Desulfotomaculum... Chung phat trien dugc nhd vao<br /> ngudn nang lugng thu dugc tU phan Crng oxy hoa cac chat huu ea ed trong nude nhu Lactat, Ethanol, Methanol,<br /> acid Diearboxylie... va phan Ung khCr S04^' thanh H2S [1].<br /> <br /> 2C + SO^^- + 2H2O ^ ' ^ ^ ' ^ ' " ^ ^ % 2HC03^- + H^S<br /> (Vat chat huu ca)<br /> <br /> Nhiet do thich hgp cho hau het cac vi khuan khCr sunfat la 20°CH- 4 0 ° C . Tuy nhien cung cd mdt vai loai ed<br /> the chiu dugc nhiet do tU 65°CH-70°C, vidu nhu Desulfotomaculum nigrificants. Vi khuan SRB chiu nhiet khdng<br /> phat hien thay trong nude bien md Bach Hd [2]<br /> <br /> Trong mdi trudng nude, vi khuan khu Sunfat SRB tdn tai d hai dang: bai Idi va bam dinh.<br /> <br /> Vi khuan SRB bam dinh tren be mat kim loai (be mat dudng dng d i n dau nude) tao ra mdt mang sinh<br /> hgc bao gdm cac Polysaeharit, enzim va ion kim loai (Mg, Fe, Ca...) dugc ggi la mang Biofilm. Chinh mang<br /> Biofilm nay la nai cu ngu va phat then cua vi khuan SRB bam dinh. Su tao thanh mang Biofilm trong dudng dng<br /> lam giam kha nang trao ddi nhiet eua cac dng dan, lam giam luu lugng ddng chay va dac biet la gay an mdn<br /> diem rat khd phat hien.<br /> <br /> Oe giam eae tdn that ke tren, nude bien hoae nude via trudc khi bam ep xudng via dugc xu ly bang cac<br /> chat diet khuan de kiem soat su phat trien eua vi khuan SRB. Tuy nhien can luu y hieu qua diet vi khuan bam<br /> dinh cua eae chat diet khuan thugng nhd han so vgi diet vi khuan bai lgi. Cac chat diet vi khuan SRB dugc chia<br /> lam hai nhdm ehinh [3]:<br /> <br /> • Chat diet khuan oxy hoa gdm: Hydro Peroxyt H2O2, Cloro Dioxyt CIO2. Chat diet khuan loai nay dugc<br /> sCr dijng vgi ndng do 1500^5000 ppm theo trgng lugng.<br /> <br /> • Chat diet khuan huu ea khdng oxy hoa gdm:<br /> <br /> <br /> <br /> 164<br />  Hdi thdo khoa hoc "Nang cao he sd thu hdi ddu md Bach Ho"<br /> <br /> <br /> <br /> + Cac hgp chat aldehyde nhu:<br /> <br /> Acrolein (CH2=CH-CH0)<br /> <br /> Glutaraldehyde (CH0-(CH2)3-CH0)<br /> <br /> + Cac hap chat amin nhu: amin vong Tnazin, cac amin bae bd'n, Amin th^ng,,,<br /> <br /> + Cac hgp chat Thiocacbamat, Thiazolone...<br /> <br /> Ndng dp chat diet khuan huu ea khdng oxy hoa de xCr ly nude tU 50ppm den 500ppm,<br /> <br /> Trong sd cac chat diet khuan ke tren thi acrolein cd hieu qua nhat. Tuy nhien acrolein cung cd han che la<br /> de bi polymer hoa va ed mui hae rat khd chiu gay khd khan cho ngudi sCr dung.<br /> <br /> Vdi muc dich tim kiem cac chat thay the chat diet khuan eua nude ngoai dang su dung d md Bach Hd,<br /> chung tdi da nghien eUu che tao trong phdng thi nghiem 3 loai diet khuan sau:<br /> <br /> - Chat A tren ca sd hgp chat amin vdng (Thazine).<br /> <br /> - Chat B tren ea sd hgp chat ngung tu cua acrolein vdi formaldehyde.<br /> <br /> - Chat C tren ca sd ket hap eae aldehyde, chat HDBM loai Nonyl phenol polyethylen oxyt va hgp<br /> chat Amin.<br /> <br /> Hieu qua diet khuan eua eae chat nay da duac danh gia va so sanh vdi Troskin 5407, va Aeticide DDQ<br /> 50 (hang Benh-Bayer).<br /> <br /> I-PHLTONG P H A P D I ^ U C H ^<br /> <br /> 1.1-Oieu che Thazine (1,3,5 tri Hydroxylethyl Hexahydro-S-triazin) [2,3]<br /> <br /> G2H4OH<br /> <br /> <br /> <br /> H2N-C2H4OH + HCHO — ^ I 1<br /> (C2H40H)-N N-C2H4OH<br /> <br /> Oieu kien thue hien phan Ung:<br /> <br /> - Ty le moi monoEthanolamin/Formalin = 1/1,1<br /> -XuetacNH40H<br /> -Nhiet do 58°C±1<br /> <br /> San pham thu dugc la chat Idng mau vang, cd 60%^65% Triazine, tan trong nuge, pH * 10^10,5<br /> <br /> 1.2-Dieu che san pham ngung tu<br /> <br /> 1.2.1- Dieu che acrolein [5]:<br /> <br /> Xt K2SO4<br /> CH20H-CHOH-CH20H CHn=CH-CHO + 2H,0<br /> 170^180°C<br /> Oieu kien thuc hien phan Ung:<br /> -XuetacKHSO^<br /> -Tyle moi Glyxeryl/KHSO^* 1/1<br /> - Nhiet dd phan ung 190-200-C<br /> <br /> <br /> <br /> 165<br /> Hdi thdo khoa hoc "Ndng cao he sd thu hdi ddu md Bqch Ho"<br /> <br /> <br /> <br /> Sau khi chung eat loai nude, san pham ehCra 90% acrolein. Oe tranh acrolein bi polymer hoa, khi chung<br /> cat cho them mdt lugng nhd Hydroquinon vao acrolein.<br /> 1.2.2- Ngung tti Acrolein va Formaldehyde [6,7]<br /> <br /> Xuc tac ^^^°^<br /> (n+2)CH2=CH-CH0 + (n+3)HCH0 + H2O •<br /> OH-CH,—C —CHO<br /> CH2OH<br /> <br /> CH2—C —CHO ^<br /> Oieu kien thifc hien phan Crng:<br /> CH2OH<br /> - Ty le moi acrolein/formaldehyde = 1/1,5 I<br /> - Xuc tac Methylamine (CH3- NH2) CH2 - C - C H O<br /> <br /> - Nhiet dp phan Crng: 82^85°C CH2OH<br /> <br /> San pham cd mau vang nhat chCra 40^45% nude tach ra bang chung cat chan khdng d ap suat<br /> 5^6mmHg.<br /> 1.3- Thanh phan chat C<br /> Chat C cd thanh phan 70-80% hdn hgp eae Aldehyde tU C1 den C5, 20^30% hgp chat amin va mot<br /> phan nhd chat hoat ddng be mat.<br /> <br /> II- PHL/ONG PHAP O A N H GIA HIEU Ll/C CAC CHAT DIET KHUAN<br /> Phudng phap danh gia hieu luc cua chat diet khuan deu dua tren nguyen tac cho vi khuan tiep xuc vdi<br /> chat diet khuan d cac ndng do va thdi gian khae nhau. Sau dd kiem tra kha nang sdng sdt eua vi khuan tren mdi<br /> trudng nudi cay tdng hgp. Trong phdng thi nghiem thudng ket hgp 3 phudng phap sau de xac dinh hieu li/c chat<br /> diet khuan:<br /> 1. Phuang phap dua tren viec phan tich tdng lugng HjS cd trong miu thi nghiem bang phuang phap so<br /> mau (phuang phap HACH 8131).<br /> 2. Phuang phap dinh tinh vi khuan (dua tren su sdng sdt eCia vi khuan sau khi tiep xuc vdi chat diet khuan<br /> thdng qua su xuat hien mau den tren mdi trudng dinh dudng mdi theo tieu chuan API).<br /> 3. Phuang phap dinh lugng vi khuan (dua tren su dem sd te bao vi khuan cd trong 1ml mau trudc va sau<br /> khi cho chat diet khuan bang phuang phap pha loang theo tieu chuan API). Bang phuang phap nay<br /> cung ed the xac dinh dugc sd vi khuan bi diet (tinh theo %).<br /> 11.1- Danh gia hieu luc chat diet khuan doi vdi hon hdp vi khuan SRB thong qua xac djnh nong do HjS [8]<br /> Cho vao Ig penieilin da khCr trung (dung tich 13ml) theo thu tu: 9 ml nude bien cd chCra chat diet khuan,<br /> 2ml mdi trudng dinh dudng va 1ml dung ed chCra vi khuan SRB vdi ham lugng 10^-^-1 O^TB/ml. Sau dd day kin<br /> bang nut cao su, han che tdi da khdng khi lot vao. U am cac Ig thi nghiem d nhiet do 30°C±1. Su phat trien cua<br /> vi khuan sau thgi gian 7 ngay dugc danh gia bang lugng H2S sinh ra va su xuat hien mau den trong ket tua d<br /> day cac Ig thi nghiem.<br /> <br /> HjS xac dinh theo phuang phap so mau tren giay HACH-HS-C, Hydrogen Sunfide Test Kit - Model<br /> HS-C-2537800 [8].<br /> Ngudn vi khuan khCr Sunfat phan lap tir nude via va lam giau trong mdi trudng dinh dudng theo phudng<br /> phap [9,10].<br /> <br /> <br /> 166<br />  Hdi thdo khoa hoc "Ndng cao he sdthu hdi ddu md Bqch Hd "<br /> <br /> <br /> <br /> Mdi trudng cho vi khuan khCr Sunfat phat trien (mdi trudng dinh dudng) ed thanh phan [10,11]:<br /> Lactat Natri: 4,0ml<br /> Cao nam men: 1,0g<br /> ThioglicolicAcid: 0,1g<br /> <br /> MgS04.7H20: 0,2g<br /> K2HPO4 (khan): 0,1g<br /> Mudi Morh FeS04(NH4)2S04.6H20: 0,1g<br /> CaCl2.2H20: 0,1g<br /> NaCI: lO.Og<br /> Cac thanh phan tren pha trong 1000ml nude cat, pH mdi trudng dugc dieu ehinh bang NaOH tdi pH 7,5.<br /> 11.2 - Xac dinh kha nang diet vi khuan khLf Sunfat bam dinh va bdi loi theo phi/dng phap API [11]<br /> 11.2.1 - Xac dinh kha nang diet nhdm vi khuan khCr Sunfat bam dinh<br /> Ngam thanh thep da dugc lam sach be mat (danh bdng be mat, tay md, khu trung) vao mdi trudng dinh<br /> dudng ed hdn hgp vi khuan khCr Sunfat trong thgi gian 2^3 tuan cho den khi bi bao phu mau den (mang Biofilm).<br /> Chuyen thanh thep vao mdi trudng nude bien cd ehUa chat diet khuan. Thdi gian thanh thep tiep xuc vdi chat<br /> diet khuan la 1 ngay, 4 ngay, 7 ngay. Sau dd lay thanh thep ra va ngam vao mdi trudng dinh dudng, quan sat su<br /> xuat hien mau den eua can day. Tat ca cac thi nghiem tren deu thue hien d 30°C. Neu cd mau den chUng td vi<br /> khuan bam dinh vin chua bi diet.<br /> <br /> Bang phuang phap nay cung ed the xac dinh dugc ndng do chat diet khuan nhd nhat de diet vi<br /> khuan SRB.<br /> <br /> 11.2.2- Xac dinh kha nang diet nhdm vi khuan khCr Sunfat bai loi<br /> <br /> Ngam thanh thep da dugc lam sach be mat vao mdi trudng dinh dudng ed chCra hdn hgp vi khuan khCr<br /> Sunfat, bao quan miu d nhiet do 30°C±1 trong hai den 3 tuan. Lay thanh thep bi phu mang den ra, can cdn lai<br /> trong Ig thi nghiem chUa ehCi yeu la vi khuan bai Idi. Lay 1ml can nay cay vao nude bien ehUa chat diet khuan.<br /> Bao quan mau d nhiet dp 30°C. Sau 1 ngay, 4 ngay, 7 ngay lay can day eua nhung Ig nay cay vao mdi trudng<br /> dinh dudng. Theo ddi su phat trien trd lai eua vi khuan bang each quan sat su xuat hien mau den d cac Ig thi<br /> nghiem. Xac dinh sd vi khuan bdi Idi cdn lai bang each pha loang va so sanh vdi miu ddi ehirng. Tren ed sd nay<br /> tinh dugc % vi khuan bi diet:<br /> <br /> Sd vi khuan ban dau - Sd vi khuan cdn lai<br /> % Vi khuan bi diet = ; ^ '•— x 100% (1)<br /> Sd vi khuan ban dau<br /> <br /> Neu 1 hoa chat co kha nang diet > 99% so vi khuan so vdi ban dau a mot nong do nao do co the duac<br /> xem ta chat diet khuan a ndng do dd.<br /> <br /> Ill- cAc K^T QuA vA THAO LUAN<br /> Ndng do H2S trong cac mau ed chat diet khuan d ndng do 50ppm, lOOppm, 200ppm bao quan d nhiet<br /> dp 30°±1 trong sudt 7 ngay da dugc xac dinh va trinh bay trong Bang 1. Ket qua nay cung dugc minh hoa<br /> bang Od thi 1.<br /> <br /> <br /> <br /> 167<br /> Hdi thdo khoa hoc "Ndng cao he sdthu hdi ddu md Bqch Ho"<br /> <br /> <br /> <br /> Bang 1: Ndng dp H2S trong eae m i u sau 1 tuan xu ly vdi chat DK<br /> <br /> Nong do H2S sinh ra (ppm)<br /> STT Chat diet khuan<br /> 50 100 200<br /> 1 M i u ddi chCfng 450 450 450<br /> 2 1 Troskin 5407 20 5 0<br /> 3 Aeticide DDQ50 120 75 5<br /> 4 ! A 100 50 10<br /> 5 B 135 65 1<br /> 6 C 25 10 0<br /> Ghi chu: Troskin5407 la chat diet khuan Viet X6 dang dung<br /> Aeticide DDQ50 la chat diet vi khuan SRB cua hang Benh-Baye<br /> <br /> <br /> 45U 1 1 j i*<br /> <br /> <br /> <br /> '^^n - — Doi chirng<br /> w<br /> 'xnn - — Troskin<br /> a. LJ<br /> a..<br /> 9^n -<br /> \ \\ --.- - Aeticide<br /> V \^<br /> lOf) -<br /> \ \A<br /> A,<br /> 1 sn - \ \\ U -A<br /> <br /> 1 no - V YL * *'^^**^.<br /> 0<br /> yj -B<br /> ^0 - \ 1"^^^^:^^^^--.<br /> - -X- - -c<br /> 0 -<br /> 50 100 150 200<br /> Nung do chat diet khuan (ppm)<br /> <br /> 0 6 thi 1: Sir phu thudc eua ndng dp H2S sinh ra do su ed mat<br /> eua vi khuan SRB vao ndng do thudc diet khuan sau 1 tuan xCr ly<br /> <br /> TCr eae sd lieu trong Bang 1 nhan thay d miu ddi ehCrng (khdng ed chat diet khuan) sau 7 ngay lugng H2S<br /> sinh ra do dugc 450ppm. Oieu nay chUng td trong mdi trudng dinh dudng, hdn hgp vi khuan khCr sunfat phat<br /> trien rat manh (tai thgi diem ban dau lugng H2S do dugc khoang 5ppm^10ppm). Su phat sinh khi H2S do ket<br /> qua hd hap trong dieu kien thieu oxy eua vi khuan khu sunfat da lam xCie tac cho phan Ung:<br /> <br /> Vi khuan SRB<br /> 2C + SO4'- + 2H2O 2HCO3'- + HjS<br /> (Vat chat huu ea)<br /> <br /> Sir cd mat chat diet khuan da lam giam lugng H2S trong m i u nghien eCru. Theo chieu tang ndng dp<br /> chat diet khuan tU 50ppm den 200ppm, lugng H2S sinh ra do dugc giam manh (xem Od thi 1). Dae biet d<br /> ndng do 200ppm, tat ca cac chat diet khuan nghien cuu deu ed hieu qua Cre che hinh thanh Hydro Sunfua.<br /> Ndng dp H2S do dugc nam trong ngudng tU On-IOppm. Hoat tinh kim ham H2S cua cac chat d ndng dp lOOppm<br /> giam theo thCftu sau:<br /> <br /> <br /> <br /> 168<br />  Hdi thdo khoa hoc "Ndng cao he sdthu hdi ddu md Bqch Hd "<br /> <br /> <br /> <br /> THOI GIAN VI KHUAN BAM DINH BI ITC CHE PHU THUOC<br /> VAO NONG DO CHAT DIET KHUAN (SAU 1 NGAY TIEP XIJC V(3l CHAT DIET KHUAN)<br /> <br /> u 7<br /> •o<br /> b<br /> ._,<br /> X!<br /> 6<br /> C<br /> ••a 5<br /> 3<br /> JZ .^.<br /> St 4<br /> c<br /> > 3<br /> c<br /> n T<br /> 01<br /> ;5 1<br /> t-<br /> 0<br /> Oppm 50ppm lOOppm 200ppm<br /> Nong do chat diet khuan (ppm)<br /> <br /> <br /> THC)I GIAN VI KHUAN BAM DINH BI iTC CHE PHU THUOC<br /> VAO NONG DO CHAT DIET KHUAN (SAU 4 NGAY TIEP XUC V6l CHAT DIET KHUAN)<br /> <br /> <br /> <br /> o ElTroskin<br /> •a<br /> c<br /> ••a<br /> S Aeticide<br /> 3<br /> iHA<br /> c<br /> C<br /> WB<br /> S<br /> 'si HC<br /> H<br /> <br /> Oppm 50ppm lOOppm 200ppm<br /> Nong do chat diet khuan (ppm)<br /> <br /> <br /> THOI GIAN VI KHUAN BAM DINH BI UC CHE PHU THUOC<br /> VAO NONG DO CHAT DIET KHUAN (SAU 7 NGAY TIEP XUC V6l CHAT DIET KHUAN)<br /> <br /> <br /> <br /> ^Troskin<br /> <br /> c H Aeticide<br /> ••rl<br /> 3 DA<br /> c<br /> > mB<br /> c<br /> .2 HC<br /> '»b<br /> :5<br /> <br /> <br /> Oppm 50ppm lOOppm 200ppm<br /> Nong dp chat diet khuan (ppm)<br /> <br /> <br /> <br /> <br /> 169<br /> Hdi thdo khoa hoc "Ndng cao he sd thu hdi ddu md Bach Ho'<br /> <br /> <br /> <br /> Troskin 5407 > C > A > B > Aeticide DDQ50<br /> Rieng d ndng do 50ppm ed the nhan xet la ehi cd Troskin 5407 va chat C cd tac dung ure che sinh ra<br /> HjS. Oieu nay cung dugc xac minh bang viec quan sat can day eae miu ehUa Troskin 5407 va C thi trang<br /> edn eae miu chua Aeticide DDQ50, A va B thi den sau 7 ngay Ci am (30"C). Mau den d can day ehinh la FeS<br /> tao thanh tU phan Crng sau:<br /> <br /> Fe'^ + H2S ^ FeS4 + 2H^ '. :'<br /> Oe xac dinh ehinh xac hieu luc diet vi khuan khCr Sunfat cua cac chat nghien eCru, da tien hanh danh gia<br /> kha nang diet vi khuan bam dinh va vi khuan bdi Idi. Cac sd lieu ve diet vi khuan bam dinh la ea sd quan trgng<br /> de danh gia hieu luc eua chat diet khuan vi diet vi khuan bam dinh khd hdn nhieu so vdi vi khuan bdi Idi. Tren<br /> bieu dd I, II, III trinh bay thdi gian vi khuan khu Sunfat bam dinh bi dc che (khdng hoat ddng) phu thudc vao<br /> ndng do chat diet khuan vdi thdi gian tiep xuc 1 ngay, 4 ngay va 7 ngay (Od thi 2). Nhin vao cac bieu dd nay<br /> nhan thay sau 1 ngay tiep xCie d ndng dp 50ppm ehi ed Troskin 5407 va C la cd hieu qua diet vi khuan bam dinh<br /> (thdi gian bi uc che 2 ngay). Cac chat diet khuan edn lai ehi bat dau ed hieu qua d lOOppm nhung d mCfc dp<br /> yeu. Gia tang thdi gian tiep xCie lam cho kha nang diet vi khuan bam dinh cua eae chat dugc cai thien rat nhieu.<br /> Sau 4 ngay tiep xuc d ndng dp lOOppm, thdi gian vi khuan bam dinh bi Cre che tang gan gap 2 lan ddi vdi<br /> Troskin 5407 va C, 3 lan ddi vdi cac chat edn lai so vdi 1 ngay tiep xuc. 6 cac ndng do va thgi gian tiep xuc khae<br /> nhau, hoat tinh cua eae chat deu tuan theo thU tu sau:<br /> <br /> Troskin 5407 > C > Aeticide DDQ50 > A, B<br /> Hoat tinh diet vi khuan bai Idi eua eae chat diet khuan cGng da dugc xac dinh theo phuang phap 11.3.2.<br /> Cac miu thi nghiem vai ndng dp chat diet khuan 50ppm, lOOppm, 200ppm sau 1 ngay va 4 ngay Ci am d 30°C±1<br /> (chinh la thgi gian tiep xuc) da dugc xem xet. Cac ket qua dugc trinh bay tren Od thi 3, 4 va Bang 2.<br /> TU cac sd lieu trong Bang 2 dd dang nhan thay hieu qua diet vi khuan bai Idi eua eae chat nghien cuu<br /> deu rat cao. Ngay d ndng do 50ppm, ehi trong 1 ngay tiep xuc, hieu qua diet khuan eua eae chat da dat dugc d<br /> mCre > 90%. Cung tu day thay rang de diet hoan toan vi khuan bai Idi ddi vdi chat diet khuan Troskin 5407 va C<br /> chi can ndng do lOOppm, vai cac chat cdn lai la 200ppm va thdi gian tiep xCie la 24h.<br /> <br /> <br /> <br /> <br /> - - O - - Troskin<br /> <br /> Acticid<br /> <br /> <br /> <br /> <br /> — r 1 1 —<br /> <br /> 50 100 150 200<br /> Nong dp chat diet khuan (ppm)<br /> <br /> <br /> Do thi 3: Hieu qua diet vi khuan bai Idi phu thudc vao ndng do chat diet khuan<br /> (thdi gian tiep xCie 1 ngay)<br /> <br /> <br /> <br /> 170<br />  Hdi thdo khoa hoc "Ndng cao he sdthu hdi ddu md Bqch Hd "<br /> <br /> <br /> <br /> <br /> 50 100 150<br /> Noi^i' do chat diet khuan (ppm)<br /> <br /> <br /> Do thi 4: Hieu qua diet vi khuan bai Idi phu thudc vao ndng do chat diet khuan<br /> (thai gian tiep xCie 4 ngay)<br /> Bang 2.<br /> Hieu qua diet khuan (%) *<br /> TT Chat diet khuan Thcfi(jian tiep xuc' ngay Thdi(jian tiep xuc 4 ngay<br /> SOppm lOOppm 200ppm SOppm lOOppm 200ppm<br /> 1 Troskin 5407 99,90 99,99 >99,99 >99,99 >99,99 >99,99<br /> 2 Aeticide DDQ50 90,90 99,90 ; >99,99 ^ 99,00 99,90 >99,99<br /> 3 A 90,00 99,90 1 >99,99 1 99,00 >99,99 >99,99<br /> 4 B 90,00 99,90 1 >99,99 : 99,90 >99,99 >99,99<br /> 5 C 99,00 99,99 ' >99,99 1 99,90 >99,99 >99,99<br /> <br /> * Hieu qua diet vi khuan bai loi tinh theo cong thuc (1).<br /> <br /> IV- K^'T L U A N<br /> <br /> 1 - Cac chat diet khuan da che tao trong phdng thi nghiem A, B, C deu ed kha nang uc che hinh thanh<br /> HjS va hoat tinh diet vi khuan khu sunfat cao, cd the dung de xCr ly nude bam ep nham muc dich nang cao thu<br /> hdi dau. Trong sd dd chat C cd hoat tinh tuang duang vdi Troskin 5407, chat A va B tuang duang vdi Aeticide<br /> DDQ50 (hang Benh Bayer).<br /> <br /> 2 - Ndng dp hieu qua de diet vi khuan khCr sunfat (bdi lgi va bam dinh) ddi vdi:<br /> -ChatC: lOOppm<br /> - Chat A va B: 200ppm<br /> <br /> 3 - Hieu qua diet vi khuan SRB phu thudc nhieu vao thdi gian tiep xCie vdi chat diet khuan. Odi vdi chat C,<br /> d ndng do lOOppm sau 4 ngay tiep xuc se diet dugc gan het (>99,99%) lugng vi khuan bai Idi ban dau va sau 7<br /> ngay tiep xuc da Uc che dugc hoan toan su phat sinh khi HjS. Oieu nay cd nghla la sau 7 ngay tiep xCie, chat C<br /> hoan toan ed kha nang diet va Uc che hdn hgp vi khuan SRB d ndng dp lOOppm.<br /> <br /> <br /> <br /> 171<br /> Hoilltao khoa hoc "Nang cao he so thu hdi ddu mo Bach Ho"<br /> <br /> <br /> <br /> TAI Lieu THAM KHAO<br /> <br /> 1. R.Cord-Ruwisch, W.KIemitz,<br /> F,Widdel: "Sunfate reducing<br /> Bactena and their activities in Oil<br /> Production" - Journal Petroleum<br /> mm<br /> t? it<br /> Technology, January 1987-97-106, 4 5^5*<br /> <br /> 2. Eri-l\")3aH0Ba. I'l.A. Bop^eii-<br /> KOB... MnK'po6iio.iornH - 2001. \<br /> TOM 7 0 N - 1 , 118-127,<br /> k<br /> 3.<br /> 4,<br /> US Patent 4,802,994,<br /> Cao Anh Dung, Tran Xuan<br /> Phuang, Le Minh San, Le Anh<br /> Dao, Trinh Viet Thang, Than<br /> Hoang Cudng, Nguyen Thi Thai-<br /> Bao cao de tai nghien cuu cap<br /> nganh "Nghien cuu che tao vat<br /> i%x\.\<br /> Miu thep co vi khuan khu Sunfatb bam dinh da duac xd ly vai<br /> chat diet khuan C (ky hieu N19) d cac nong dd SOppm, lOOppm<br /> lieu va giai phap cdng nghe lam va 200ppm (thai gian tiep xuc 4 ngay) sau 3 ngay quan sat.<br /> sach H2S trong khi ddng hanh<br /> md Bach Ho"-Ha N61 7-2-2001,<br /> trang 32-^37,<br /> <br /> Tai lieu neng eua PGS,TS Hoa<br /> Huu Thu - Trudng Dai hoc Tdng<br /> hap Ha Npi,<br /> US Patent 4,479,820,<br /> US Patent 2,696,477,<br /> [8]: C K /Kill \cHOBa. l^.b,<br /> Po5nh iipnK.iaoiiiia>i 6iio\ii\iii>i<br /> H ,\lHK'po6llO.'Kiril^ IO\l.-)().<br /> N"-6, 2000 traiiLi 6