Active tuberculosis

The consequences of tuberculosis on human society are immense. Tuberculosis remains a major cause of morbidity and mortality in many countries and a significant public health problem worldwide. Active tuberculosis is diagnosed by detecting Mycobacterium tuberculosis complex bacilli in specimens from the respiratory tract (pulmonary TB) or in specimens from other bodily sites (extra pulmonary TB).

5p viuchiha 06-01-2025 0 0   Download

A series of novel 10-phenylsulfonyl-2-substituted-4,10 dihydrobenzo[4,5]imidazo[1,2- a]pyrimidin-4-one derivatives obtained from N-sulfonation of 2-substituted-pyrimido[1,2- a]benzimidazol-4(10H)-ones and screened for for their in vitro anti-tuberculosis activities against Mycobacterium tuberculosis H37Rv by Microplate Alamar Blue Assay (MABA) method.

8p tocectocec 25-05-2020 14 1   Download

The gene fprA of Mycobacterium tuberculosis, encoding a putative protein with 40% identity to mammalian adrenodoxin reductase, was expressed in Escherichia coli and the protein purified to homogeneity. The 50-kDa protein monomer contained one tightly bound FAD, whose fluorescence was fully quenched. FprA showed a low ferric reductase activity, whereas it was very active as a NAD(P)H diaphorase with dyes. Kinetic parameters were determined and the specificity constant (kcat/Km) for NADPH was two orders of magnitude larger than that of NADH....

9p system191 01-06-2013 38 3   Download

The Rv0948c gene fromMycobacterium tuberculosisH37Rv encodes a 90 amino acid protein as the natural gene product with chorismate mutase (CM) activity. The protein, 90-MtCM, exhibits Michaelis–Menten kinetics with akcat of 5.5 ± 0.2 s )1 and a Kmof 1500 ± 100lmat 37C and pH 7.5. The 2.0 A˚ X-ray structure shows that 90-MtCM is an alla-helical homodimer (Protein Data Bank ID: 2QBV) with the topology ofEscheri-chia coliCM (EcCM), and that both protomers contribute to each catalytic site.

12p research12 29-04-2013 39 3   Download

Pathogenicity ofMycobacterium tuberculosisis closely rela-ted to its ability to survive and replicate in the hostile envi-ronment of macrophages. For some pathogenic bacteria, secretion of ATP-utilizing enzymes into the extracellular environment aids in pathogen survival via P2Z receptor-mediated, ATP-induced death of infected macrophages. A component of these enzymes is nucleoside diphosphate kinase (Ndk). Thendkgenewas cloned fromM. tuberculosis H37Rv and expressed inEscherichia coli.

10p tumor12 20-04-2013 42 3   Download

The gene encompassing ORF Rv1885c with weak sequence similarity to AroQ chorismate mutases (CMs) was cloned from the genome ofMycobac-terium tuberculosis and expressed in Escherichia coli. The gene product (*MtCM) complements a CM-deficient E. colistrain, but only if produced without the predicted N-terminal signal sequence typical ofM. tuberculosis. The mature *MtCM, which was purified by exploiting its resistance to irre-versible thermal denaturation, possesses high CM activity in vitro.

15p awards 05-04-2013 36 2   Download

The Mycobacterium tuberculosisgenome contains an unusually high number of proteins involved in the metabo-lism of lipids belonging to the Lip family, including various nonlipolytic and lipolytic hydrolases. Driven by a structural genomic approach, we have biochemically characterized the Rv1399c gene product, LipH, previously annotated as a putative lipase. Rv1399c was overexpressed inE. colias inclusion bodies and refolded. Rv1399c efficiently hydro-lyzes soluble triacylglycerols and vinyl esters.

0p awards 05-04-2013 49 3   Download

The genesRv1318c, Rv1319c, Rv1320cand Rv3645 1 of Mycobacterium tuberculosisare predicted to code for four out of 15 adenylyl cyclases in this pathogen. The proteins consist of a membrane anchor, a HAMP region and a class IIIb adenylyl cyclase catalytic domain. Expression and purification of the isolated catalytic domains yielded aden-ylyl cyclase activity for all four recombinant proteins.

6p dell39 03-04-2013 35 3   Download

Phosphorylation of the mycobacterial transcriptional activator, EmbR, is essential for transcriptional regulation of theembCABoperon encoding cell wall arabinosyltransferases. This signaling pathway eventually affects the resistance to ethambutol (a frontline antimycobacterial drug) and the cell wall Lipoarabinomannan⁄Lipomannan ratio (an important determinant for averting the host immune response).

11p dell39 27-03-2013 36 2   Download

Adenylyl cyclase Rv2212 fromMycobacterium tuberculosishas a domain composition identical to the pH-sensing isoform Rv1264, an N-terminal regulatory domain and a C-terminal catalytic domain. The maximal velo-city of Rv2212 was the highest of all 10 mycobacterial cyclases investigated to date (3.9lmol cAMPÆmg )1 Æmin )1 ), whereas ATP substrate affinity was low (SC50¼2.1 mmATP).

10p inspiron33 25-03-2013 22 4   Download

The nicotinamidase⁄pyrazinamidase (PncA) ofMycobacterium tuberculosis is involved in the activation of the important front-line antituberculosis drug pyrazinamide by converting it into the active form, pyrazinoic acid. Mutations in thepncA gene cause pyrazinamide resistance in M. tuber-culosis.

10p media19 05-03-2013 34 1   Download

The characterization of membrane proteins having no identified function in Mycobacterium tuberculosisis important for a better understanding of the biology of this pathogen. In this work, the biological activity of the Rv2560 protein was characterized and evaluated.

13p media19 05-03-2013 34 2   Download

The alternative sigma factors are regulated by a phosphorylation-mediated signal transduction cascade involving anti-sigma factors and anti-anti-sigma factors. The proteins regulating Mycobacterium tuberculosissigma factor F (SigF), anti-SigF and anti-anti-SigF have been identified, but the factors catalyzing phosphorylation–dephosphorylation have not been well estab-lished.

14p vinaphone15 28-02-2013 34 2   Download

Antigen binding to the B-cell receptor (BCR) of pre-mature B lymphocytes induces their apoptotic cell death, but binding to the BCR of mature B lymphocytes triggers activation and proliferation. Binding to pre-mature B lymphocytes is thought not only to function as a mechanism to exclude B-cell clones that possess the ability to react with self-antigen, but also to act as a defense mechanism in auto-immune diseases.

20p vinaphone15 27-02-2013 37 3   Download

Tuberculosis (TB) continues to be a major cause of morbidity and mortal-ity worldwide. The increasing emergence and spread of drug-resistant TB poses a significant threat to disease control and calls for the urgent devel-opment of new drugs. The tryptophan biosynthetic pathway plays an important role in the survival of Mycobacterium tuberculosis.

11p vinaphone15 27-02-2013 48 2   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Host transcription in active and latent tuberculosis...

2p thulanh22 20-11-2011 39 2   Download

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: A DNA vaccine against tuberculosis based on the 65 kDa heat-shock protein differentially activates human macrophages and dendritic cells

0p toshiba20 12-11-2011 62 2   Download