Calli

The objective of this work is to experimentally investigate the effect of the oxygen potential on the fuel and FP chemical behaviour in conditions representative of a severe accident. More specifically, the speciation of Cs, Mo and Ba is investigated.

10p christabelhuynh 29-05-2020 9 2   Download

General objective: To get mutant flowering flowers that have more number of petals than the study samples, larger flowers and more aromatic. To evaluate growth culture technique to generate calli and shoots for irradiation of gamma ray;

31p phongtitriet000 08-08-2019 33 4   Download

In  Molecular simulation of nanoparticle diffusion at fluid interfaces molecular dynamics simulations are used to exam- ine the transport properties of a nanoparticle in both bulk solvent and at a liquid–liquid interface. Specifically it aims to address the effect of interfacial adsorption on the diffusion of nanoparticles (at low concentration).

5p hoquangbio 22-07-2015 27 2   Download

Introducing site-directed mutations in surface-exposed residues of subunit II of the heme aa3 cytochrome c oxidase of Paracoccus denitrificans, we analyze the kinetic parameters of electron transfer from reduced horse heart cytochrome c. Specifically we address the following issues: (a) which residues on oxidase contribute to the docking site for cytochrome c, (b) is an aromatic side chain required for electron entry from cytochrome c, and (c) what is the molecular basis for the previously observed biphasic reaction kinetics....

9p system191 01-06-2013 36 3   Download

The human multidrug resistance-associated protein (MRP1) is an ATP-dependent efflux pump that transports anionic conjugates, and hydrophobic compounds in a glutathione dependent manner. Similar to the other, well-characterized multidrug transporter P-gp, MRP1 comprises two nucleotide-binding domains (NBDs) in addition to transmembrane domains. However, whereas the NBDs of P-gp have been shown to be functionally equivalent, those of MRP1 differ significantly.

9p research12 01-06-2013 24 4   Download

Two genes (CM-AAT1 and CM-AAT2) with strong sequence homology (87% identity at the protein level) putatively involved in the formation of aroma volatile esters have been isolated from Charentais melon fruit. They belong to a large and highly divergent family of multifunctional plant acyl-transferases and show at most 21% identity to the only other fruit acyl-transferase characterized so far in strawberry. RT-PCR studies indicated that both genes were specifically expressed in fruit at increasing rates in the early and mid phases of ripening.

8p research12 01-06-2013 48 4   Download

Wilson disease (WD), an inherited disorder affecting copper metabolism, is characterized by hepatic cirrhosis and neuronal degeneration, which result from toxic levels of copper that accumulate in the liver and brain, respectively. We reported previously that the  1.3-kb promoter of the WD gene contains four metal response elements (MREs). Among the four MREs, MREa plays the most important role in the transcriptional activation of the WD promoter.

11p research12 01-06-2013 53 3   Download

The ÔMediterraneanÕ variant of glucose-6-phosphate dehydrogenase (G6PD) deficiency is due to the C563CT point mutation, leading to replacement of Ser with Phe at position 188, resulting in acute haemolysis triggered by oxidants. Previous work has shown increased formation of altered aspartate residues in membrane proteins during cell ageing and in response to oxidative stress in normal erythrocytes. These abnormal residues are specifically recognized by the repair enzyme L-isoaspartate (D-aspartate) protein O-methyltransferase (PCMT; EC 2.1.1.77). ...

8p research12 01-06-2013 38 4   Download

The RuvA, RuvB andRuvCproteins ofEscherichia coliact together to process Holliday junctions formed during recombination and DNA repair. RuvA has a well-de®ned DNA binding surface that is sculptured speci®cally to accommodate a Holliday junction and allow subsequent loading of RuvB and RuvC. A negatively charged pin pro-jecting from the centre limits binding of linear duplexDNA. The amino-acid sequences forming the pin are highly con-served.

9p research12 29-04-2013 39 3   Download

Nourseothricins (syn. Streptothricins), agroupof nucleoside peptides produced by several streptomycete strains, contain a polyb-lysine chain of variable length attached in amide linkage to the amino sugar moiety gulosamine of the nucleoside portion. We show that the nourseothricin-pro-ducingStreptomyces nourseicontains an enzyme (NpsA) of an apparentMr56 000 that speci®cally activatesb-lysine by adenylation but does not bind to it as a thioester.

11p research12 29-04-2013 23 4   Download

Yellow stem borer has been identified as a major insect pest of deepwater rice, causing severe yield losses. Bt gene(s) from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene(s) is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA(b) gene into embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation.

6p zingzing09 24-04-2013 54 1   Download

A number of findings have suggested the involvement of protein phosphorylation in the regulation of the epithelial Na + channel (ENaC).Arecent studyhas demonstrated that the C tails of thebandcsubunits of ENaC are subject to phosphorylation by at least three protein kinases [Shi, H., Asher, C., Chigaev, A., Yung, Y., Reuveny, E., Seger, R. & Garty, H. (2002)J. Biol. Chem.277, 13539–13547]. One of them was identified as ERK which phosphorylates bT613 andcT623 and affects the channel interaction with Nedd4....

8p research12 23-04-2013 34 1   Download

Cyclin-dependent kinase-5 (Cdk5) is a serine/threonine kinase activated by its neuron-specific activator, p35, or its truncated form, p25. It has been proposed that the deregu-lation of Cdk5 activity by association with p25 in human brain tissue disrupts the neuronal cytoskeleton and may be involved in neurodegenerative diseases such as Alzheimer’s disease.

8p research12 23-04-2013 32 2   Download

The intrachloroplastic localization of post-transcriptional gene expression steps represents one key determinant for the regulation of chloroplast development. We have character-ized an RNA binding protein of 63kDa (RBP63) from Chlamydomonas reinhardtiichloroplasts, which cofraction-ates with stromal thylakoid membranes. Solubility proper-ties suggest that RBP63is a peripheral membrane protein.

8p research12 23-04-2013 34 3   Download

We report the constitution and molecular characterization of a novel transglutaminase (EC 2.3.2.13) that starts to accumulate speci®cally in thenucleus in the star®sh (Asterina pectinifera) embryo after progression through the early blastula stage. The cDNA for the nuclear transglutaminase was clonedand the cDNA-deduced sequence de®nes a single open reading frame encoding aproteinwith737 aminoacids andapredictedmolecularmass of 83 kDa.

11p research12 23-04-2013 47 3   Download

RNA aptamers with anity for the Ras-binding domain (RBD) of Raf-1 were isolated from a degenerate pool by in vitroselection.These aptamers eciently inhibited theRas interaction with the Raf-1 RBD, and also inhibited Ras-induced Raf-1 activation in a cell-free system. The RNA aptamer with the most potent inhibitory e€ect speci®cally inhibited the RasáRaf-1 interaction and had no anity for the RBD of the RGL protein, a homolog of the Ral GDP dissociation stimulator.

8p research12 23-04-2013 37 2   Download

Ina search for genes regulated in response to cAMPwe have identified a newprotein, p76 RBE , whosemRNAand protein expression is enhanced in thyrocytes following thyrotropin stimulation of the cAMP transduction cascade. This protein presents important similarities with Rhophilin and contains different protein–protein interactionmotifs. The presence of HR1 and PDZ motifs as well as a potential PDZ binding domain motif suggests that p76 RBE could be implicated in targeting or scaffolding processes....

9p tumor12 22-04-2013 47 2   Download

Keyhole limpet (Megathura crenulata) hemocyanin (KLH), an extracellular respiratory protein, is widely used as hapten carrier and immune stimulant. Although it is generally accepted that the sugar constituents of this glycoprotein are likely to be implicated in the antigenicity and biomedical properties of KLH, knowledge of its carbohydrate structure is still limited. Therefore, we have investigated the N-linked oligosaccharides of KLH. Glycan chains were enzymati-cally liberated from tryptic glycopeptides, pyridylaminated and separated by two-dimensional HPLC....

15p tumor12 22-04-2013 32 2   Download

TheAspergillus nigerb-1,4-endogalactanase encoding gene (galA) was cloned and characterized. The expression ofgalA in A. nigerwas only detected in the presence of sugar beet pectin,D-galacturonic acid andL-arabinose, suggesting that galAis coregulatedwithboth thepectinolyticgenes aswell as the arabinanolytic genes. The corresponding enzyme, endogalactanase A (GALA), contains both active site resi-dues identified previously for thePseudomonas fluorescens b-1,4-endogalactanase.

9p tumor12 22-04-2013 22 3   Download

GToligomers, showingadose-dependent cytotoxic effect on a variety of human cancer cell lines, but not on normal human lymphocytes, recognize and form complexes with nuclear proteins.By working with human T-lymphoblastic CCRF-CEM cells and by using MS and SouthWestern blotting, we identified eukaryotic elongation factor 1 alpha (eEF1A) as the main nuclear protein that specifically recognizes these oligonucleotides.Western blotting and supershift assays confirmed the nature of this protein and its involvement in forming a cytotoxicity-related complex (CRC)....

12p tumor12 20-04-2013 34 1   Download