Conformance

In this Masters thesis computational modelling techniques were employed to investigate iron-free apo-Lactoferrin (apo-Lf) structural conformation changes in the presence of variant temperature and pH. These conditions represent the environment most milk protein goes through in food processing and the production of food products.

162p runthenight04 02-02-2023 8 3   Download

Nội dung của Luận văn này nhằm giới thiệu những kiến thức cơ bản về ung thư, cơ sở sinh học và vật lý trong ung thư, hệ thống máy gia tốc và hệ MLC, các bước tiến hành của quy trình điều trị ung thư theo hình dạng khối u 3D-CRT. Mời các bạn cùng tham khảo nội dung chi tiết.

115p closefriend03 13-10-2021 50 7   Download

Mục tiêu nghiên cứu của đề tài là tìm hiểu một cách tổng quan về khai phá quy trình nghiệp vụ. Nghiên cứu một số thuật toán sử dụng trong khai phá quy trình nghiệp vụ. Qua đó so sánh, đánh giá ưu và nhược điểm của các thuật toán. Sử dụng các thuật toán và công cụ khai phá quy trình nghiệp vụ để minh họa ứng dụng khai phá quy trình xử lý yêu cầu bồi thường tại sân bay với dữ liệu thực tế được mô tả trong tài liệu W.M.P.

26p tabicani 24-09-2021 23 3   Download

In the course of trying to understand the pathogenesis of leishmaniasis in relation to extracellular matrix (ECM) elements, laminin, a major ECM protein, has been found to bind saturably and with high affinity to a 67-kDa cell surface protein of Leishmania donovani. This interaction involves a single class of binding sites, which are ionic in nature, conformation-dependent and possibly involves sulfhydryls. Binding activity was significantly enhanced by Zn2+, an effect possibly mediated through Cys-rich zinc finger-like sequences on laminin....

8p system191 01-06-2013 41 4   Download

With the aim to distinguish between local and global conformational changes induced by trifluoroethanol in RNase A, spectroscopic and activity measurements in combination with proteolysis by unspecific proteases have been exploited for probing structural transitions of RNase A as a function of trifluoroethanol concentration. At 30% (v/v) trifluoroethanol (pH 8.0; 25 °C), circular dichroism and fluorescence spectroscopy indicate a cooperative collapse of the tertiary structure of RNase A coinciding with the loss of its enzymatic activity....

7p system191 01-06-2013 48 3   Download

Visual arrestin is converted from a ÔbasalÕ state to an ÔactivatedÕ state by interaction with the phosphorylated C-terminus of photoactivated rhodopsin (R*), but the conformational changes in arrestin that lead to activation are unknown. Small-angle X-ray scattering (SAXS) was used to investigate the solution structure of arrestin and characterize changes attendant upon activation. Wild-type arrestin forms dimers with a dissociation constant of 60 lM.

9p system191 01-06-2013 53 3   Download

P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. When the UIC2-shift was elicited by certain modulators (e.g. cyclosporin A or vinblastine, but not with verapamil or Tween 80), the subsequent binding of other monoclonal anti-Pgp Ig sharing epitopes with UIC2 (e.g. MM12.10) was abolished [Nagy, H., Goda, K., Arceci, R., Cianfriglia, M., ´ Mechetner, E. & Szabo Jr, G.

6p system191 01-06-2013 62 7   Download

Departamento de Bioquı´mica y Biologı´a Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Spain; Department of Chemistry, University of Minnesota, Minneapolis, Minnesota, USA Reduction potential determinations of K75E, E139K and E301A ferredoxin-NADP+ reductases provide valuable information concerning the factors that contribute to tune the flavin reduction potential. Thus, while E139 is not involved in such modulation, the K75 side-chain tunes the flavin potential by creating a defined environment that modulates the FAD conformation. ...

6p system191 01-06-2013 36 4   Download

The serpins are of general protein chemical interest due to their ability to undergo a large conformational change consisting of the insertion of the reactive centre loop (RCL) as strand 4 of the central b sheet A. To make space for the incoming RCL, the ‘shutter region’ opens by the b strands 3A and 5A sliding apart over the underlying a helix B. Loop insertion occurs during the formation of complexes of serpins with their target serine proteinases and during latency transition. This type of loop insertion is unique to plasminogen activator inhibitor-1 (PAI-1).

10p system191 01-06-2013 41 4   Download

The octadecaneuropeptide (ODN; QATVGDVNTDRPG LLDLK) and its C-terminal octapeptide (OP; RPGLLDLK), which exert anxiogenic activity, have been previously shown to increase intracellular calcium concentration ([Ca21]i) in cultured rat astrocytes through activation of a metabotropic receptor positively coupled to phospholipase C. It has also been found that the [D -Leu5]OP analog possesses a weak antagonistic activity. The aim of the present study was to synthesize and characterize cyclic analogs of OP and [D -Leu5]OP.

13p system191 01-06-2013 32 4   Download

To establish an infection, Yersinia pseudotuberculosis utilizes a plasmid-encoded type III secretion machine that permits the translocation of several anti-host factors into the cytosol of target eukaryotic cells. Secreted YopD is essential for this process. Pre-secretory stabilization of YopD is mediated by an interaction with its cognate chaperone, LcrH. YopD possesses LcrH binding domains located in the N-terminus and in a predicted amphipathic domain located near the C-terminus. This latter domain is also critical for Yersinia virulence. ...

10p research12 01-06-2013 48 3   Download

The phenomenon of the transformation of proteins into amyloid-fibrils is of interest, firstly, because it is closely connected to the so-called conformational diseases, many of which are hitherto incurable, and secondly, because it remains to be explained in physical terms (energetically and structurally). The process leads to fibrous aggregates in the form of extracellular amyloid plaques, neuro-fibrillary tangles and other intracytoplasmic or intranuclear inclusions.

10p research12 01-06-2013 32 4   Download

The solution structure of a synthetic mutant type I antifreeze protein (AFP I) was determined in aqueous solution at pH 7.0 using nuclear magnetic resonance (NMR) spectroscopy. The mutations comprised the replacement of the four Thr residues by Val and the introduction of two additional Lys-Glu salt bridges. The antifreeze activity of this mutant peptide, VVVV2KE, has been previously shown to be similar to that of the wild type protein, HPLC6 (defined here as TTTT).

8p research12 01-06-2013 36 4   Download

Calcitonin (Ct) is a 32-residue peptide hormone that is mainly known for its hypocalcemic effect and the inhibition of bone resorption. Our previous studies have led to potent, side-chain lactam-bridged human Ct (hCt) analogues [Kapurniotu, A. Kayed, R., Taylor, J.W. & Voelter W. (1999) Eur. J. Biochem.265, 606±618; Kapurniotu, A. & Taylor, J.W. (1995)J. Med. Chem.38, 836±847]. We have hypothesized that a possibly type Ibturn/bsheet confor-mation in the region 17±21 may play an important role in hCt bioactivity. ...

12p research12 01-06-2013 46 5   Download

An interface is a way to describe what classes should do, without specifying how they should do it. It’s not a class but a set of requirements for classes that want to conform to the interface E.g. public interface Comparable { int compareTo(Object otherObject); } this requires that any class implementing the Comparable interface contains a compareTo method, and this method must take an Object parameter and return an integer

19p huanltgc00061 05-05-2013 61 5   Download

Mucin glycoproteins on breast cancer cells carry shortened carbohydrate chains. These partially deglycosylatedmucin 1 (MUC-1) structures are recognized by the monoclonal antibody SM3, which is being tested for its diagnostic utility. We used NMR spectroscopy to analyze the binding mode and thebindingepitopeof peptideandglycopeptideantigens to the SM3 antibody. The pentapeptide PDTRP and the glycopentapeptide PDT(O-a-D-GalNAc)RP are known lig-ands of the monoclonal antibody.

12p research12 29-04-2013 40 3   Download

Glycosylatedpolyenemacrolide antibiotics, as nystatins and amphotericins, are amphiphilic structures known to exert antifungal activity by disrupting the fungal cell membrane, leading to leakage of cellular materials, and cell death. This membrane disruption is strongly influenced by the presence and the exact nature of the membrane sterols.

9p research12 23-04-2013 39 2   Download

Membrane-bound transhydrogenases are conformationally driven proton-pumps which couple an inward proton translocation to the reversible reduction of NADP + by NADH(forward reaction). This reaction is stimulatedby an electrochemical protongradient,Dp, presumably throughan increased release of NADPH. The enzymes have three domains: domain II spans the membrane, while domain I and III are hydrophilic and contain the binding sites for NAD(H) andNADP(H), respectively.

11p research12 23-04-2013 35 3   Download

Vibrio choleraehemolysin (HlyA), a water-soluble protein with a native monomeric relative molecular mass of 65 000, forms transmembrane pentameric channels in target bio-membranes.TheHlyAbinds to lipid vesicles nonspecifically and without saturation; however, self-assembly is triggered specifically by cholesterol.Here we show that the HlyA partitioned quantitatively to amphiphilic media irrespective of their compositions, indicating that the toxin had an amphiphilic surface.

8p research12 23-04-2013 56 4   Download

The characterization of the gene encodingLeishmania donovaniphosphofructokinase (PFK) and the biochemical properties of the expressedenzyme are reported.L. donovani has a singlePFKgene copyperhaploidgenome that encodes a polypeptide with a deducedmolecular mass of 53 988 and a pI of 9.26. The predicted amino acid sequence contains a C-terminal tripeptide that conforms to an established signal for glycosome targeting.

12p research12 23-04-2013 62 3   Download