Essential amino acid

The present study was designed to evaluate the amino acid profiles and anti-nutritional contents of six wild vegetables viz. Cardamine hirsuta, Melothria perpusilla, Cryptolepis sinensis, Persicaria chinensis, Lippia javanica and Polygonum perfoliatum from Assam, India.

8p tocectocec 25-05-2020 6 0   Download

Objective of study determining diet formula for 4-breeds exotic hybrid with optimal protein level bases on balancing some essential amino acids includes: lysine, methionine and threonine in order to improve grow capacity, feed utilization efficiency and to reduce urinal and faecal nitrogen and phosphorus excretion; formulating some formulas base on the local ingredients with optimal protein and amino acids level to ensure production efficiency of exotic 4-breeds hybrid and to reduce environment pollution in pig production.

25p nguyenthiminh32 12-07-2014 66 7   Download

The N-terminal domain (NTD) and the ligand-binding domain (LBD) of the androgen receptor (AR) exhibit a ligand–dependent interaction (N/Cinteraction). Amino acids 3–36 in theNTD(AR3)36) play a dominant role in this interaction. Previously, it has been shown that a FxxFF motif inAR3)36 , 23 FxxLF 27 , is essential forLBDinteraction. We demonstrate in the current study that AR3)36 can be subdivided into two functionally distinct fragments: AR3)13 andAR16)36.AR3)13does not directly interact with the AR LBD, but rather contributes to the transactivation function of theAR.NTD-AR.LBDcomplex....

12p tumor12 22-04-2013 29 2   Download

Era is a highly conserved GTPase essential for bacterial growth. The N-terminal part of Era contains a conserved GTPase domain, whereas the C-terminal part of the protein contains anRNA- andmembrane-binding domain, theKH domain. To investigate whether the binding of Era to 16S rRNA and membrane requires its GTPase activity and whether the GTPase domain is essential for these acti-vities, the N- and C-terminal parts of the Streptococ-cus pneumoniaeEra –Era-N(aminoacids 1–185) andEra-C (amino acids 141–299), respectively – were expressed and purified. ...

9p tumor12 20-04-2013 32 3   Download

Dipeptidyl peptidase IV (DP IV, CD26) plays an essential role in the activation and proliferation of lymphocytes, which is shown by the immunosuppressive effects of syn-thetic DP IV inhibitors. Similarly, both human immuno-deficiency virus-1 (HIV-1) Tat protein and the N-terminal peptide Tat(1–9) inhibit DP IV activity and T cell prolifer-ation. Therefore, the N-terminal amino acid sequence of HIV-1 Tat is important for the inhibition of DP IV.

10p fptmusic 16-04-2013 32 4   Download

Many pharmacologically important agents are assembled on multimodular nonribosomal peptide synthetases (NRPSs) whose modules comprise a set of core domains with all essential catalytic functions necessary for the incorporation and modification of one building block. Very often, d-amino acids are found in such products which, with few exceptions, are generated by the action of NRPS integrated epimerization (E) domains that alter the stereochemistry of the corresponding peptidyl carrier protein (PCP) bound l-intermediate....

15p fptmusic 12-04-2013 30 2   Download

Glutamine is an essential amino acid for enterocytes, especially in states of critical illness and injury. In several studies it has been speculated that the beneficial effects of glutamine are dependent on the route of supply (lumi-nal or systemic). The aim of this study was to investigate the relevance of both routes of glutamine delivery toin vitro intestinal cells and to explore the molecular basis for proposed beneficial glutamine effects: (a) by deter-mining the relative uptake of radiolabelled glutamine in Caco-2 cells;...

15p fptmusic 11-04-2013 46 2   Download

Glutamine is an essential amino acid in cancer cells and is required for the growth of many other cell types. Glutami-nase activity is positively correlated with malignancy in tumours andwith growth rate in normal cells. In the present work, Ehrlich ascites tumour cells, and their derivative, 0.28AS-2 cells, expressing antisense glutaminase mRNA, were assayed for apoptosis induced by methotrexate and hydrogen peroxide. It is shown that Ehrlich ascites tumour cells, expressing antisense mRNA for glutaminase, contain lower levels of glutathione than normal ascites cells. In addition, 0.

9p awards 05-04-2013 47 4   Download

In the postgenomic era it is essential that protein sequences are annotated correctly in order to help in the assignment of their putative functions. Over 1300 proteins in current pro-tein sequence databases are predicted to contain a PAS domainbaseduponaminoacid sequence alignments.Oneof theproblemswith the current annotationof thePASdomain is that this domain exhibits limited similarity at the amino acid sequence level.

11p dell39 03-04-2013 37 5   Download

Methionine (Met) is an essential amino acid and the only sub-strate for synthesis of S-adenosylmethionine (AdoMet) that is the main substrate for multiple intracellular methylases. There are two modes of Met metabolism in liver. In case of its dietary restriction Met can be metabolized via conservative remethylation cycle. In case of Met excess (high [Met]) it is mostly converted to cysteine via transsulfuration pathway.

291p inspiron33 26-03-2013 155 5   Download

Eukaryotic translation initiation factor 5A (eIF5A) is a protein that is highly conserved and essential for cell viability. This factor is the only pro-tein known to contain the unique and essential amino acid residue hypu-sine. This work focused on the structural and functional characterization ofSaccharomyces cerevisiaeeIF5A.

15p media19 06-03-2013 50 3   Download

Yeast elongation factor 2 is an essential protein that contains two highly conserved threonine residues, T56 and T58, that could potentially be phos-phorylated by the Rck2 kinase in response to environmental stress. The importance of residues T56 and T58 for elongation factor 2 function in yeast was studied using site directed mutagenesis and functional comple-mentation.

13p media19 04-03-2013 38 3   Download

Transglutaminase 1 (TGase 1) is an essential enzyme for cornified enve-lope formation in stratified squamous epithelia. This enzyme catalyzes the cross-linking of glutamine and lysine residues in structural proteins in differentiating keratinocytes. To gain insight into the preferred substrate structure of TGase 1, we used a phage-displayed random peptide library to screen primary amino acid sequences that are preferentially selected by human TGase 1.

11p vinaphone15 28-02-2013 32 2   Download

The ubiquitination and subsequent degradation of stress-induced abnormal proteins are indispensable to cell survival. We previously showed that a yeast (Saccharomyces cerevisiae) mutant carrying a single amino acid change, Ala401Glu, in RSP5, which encodes an essential E3 ubiquitin ligase, is hypersensitive to various stresses.

11p vinaphone15 25-02-2013 37 2   Download

The C-terminus of the fungal laccase fromMelanocarpus albomyces(MaL) is processed during secretion at a processing site conserved among the ascomycete laccases. The three-dimensional structure of MaL has been solved as one of the first complete laccase structures. According to the crystal structure of MaL, the four C-terminal amino acids of the mature protein penetrate into a tunnel leading towards the trinuclear site.

16p viettel02 20-02-2013 49 3   Download

Translation initiation factor 1 (IF1) is an essential protein in prokaryotes. The nature of IF1 interactions with the mRNA during translation initiation on the ribosome remains unclear, even though the factor has several known functions, one of them being RNA chaperone activity. In this study, we analyzed translational gene expressionin vivo in two cold-sensitive chromo-somal mutant variants of IF1 with amino acid substitutions, R40D and R69L, using two different reporter gene systems.

12p mobifone23 21-01-2013 45 3   Download

Roquin is an E3 ubiquitin ligase with a poorly understood but essential role in preventing T-cell-mediated autoimmune disease and in microRNA-mediated repression of inducible costimulator (Icos) mRNA. Roquin and its mammalian paralogue membrane-associated nucleic acid binding protein (MNAB) define a protein family distinguished by an 200 amino acid domain of unknown function, ROQ, that is highly conserved from mam-mals to invertebrates and is flanked by a RING-1 zinc finger and a CCCH zinc finger....

19p mobifone23 21-01-2013 31 4   Download

Abstract Enterovirus type 71 (EV71) 2A protease exhibited strong transcriptional activity in yeast cells. The transcriptional activity of 2A protease was independent of its protease activity. EV71 2A protease retained its transcriptional activity after truncation of 40 amino acids at the N-terminus but lost this activity after truncation of 60 amino acids at the N-terminus or deletion of 20 amino acids at the C-terminus. Thus, the acidic domain at the C-terminus of this protein is essential for its transcriptional activity.

9p toshiba23 18-11-2011 59 2   Download

Excitatory amino acid release and subsequent biochemical cascades following traumatic brain injury (TBI) have been well documented, especially glutamate-related excitotoxicity. The effects of TBI on the essential functions of inhibitory GABA-A receptors, however, are poorly understood. Methods: We used Western blot procedures to test whether in vivo TBI in rat altered the protein expression of hippocampal GABA-A receptor subunits α1, α2, α3, α5, β3, and γ2 at 3 h, 6 h, 24 h, and 7 days post-injuy.

11p toshiba23 18-11-2011 46 4   Download