Glycoproteins

Luận văn được thực hiện với mục tiêu nhằm thiết kế được vector biểu hiện protein tái tổ hợp GP5; biểu hiện và tinh sạch được protein tái tổ hợp GP5 của Porcine Reproductive and Respiratory Syndrome virus. Mời các bạn cùng tham khảo.

54p zhangyan 13-07-2021 32 5   Download

Mục tiêu luận án là đánh giá khả năng áp dụng phương pháp định lượng Hocmon PAG và Lactogen trên trâu đầm lầy Việt Nam nhằm mục đích chẩn đoán có chửa sớm và cung cấp thông tin về biến động của Hocmon này trong quá trình mang thai. Kết quả nghiên cứu về Protein Hocmon thời kỳ có chửa và biểu hiện của chúng ở trâu là một đóng góp quan trọng liên quan đến kiến thức cơ bản của sinh sản trâu đầm lầy.

27p lequangvinh1608 13-08-2019 40 1   Download

Thường sử dụng kích dục tố là HCG được chiết xuất từ nước tiểu của phụ nữ có thai hoặc từ nhau thai, bản chất hóa học là glycoprotein, do có chất lượng ổn định. Hình 3.1.7: HCG Trung Quốc sản xuất (trái) và Việt Nam sản xuất (phải). Tiêm kích dục tố vào gốc vây lưng, nghiêng một gốc 450 so với thân, hướng mũi kim về phía đầu cá, tay giữ chặt phần đầu và đuôi, khi tiêm xong thoa nhẹ chổ tiêm. Chuẩn bị kích dục tố bằng cách lấy 10cc nước muối sinh lý (9o/oo) đem pha với...

3p beepbeepnp 21-06-2013 80 5   Download

Dystrobrevin is one of the intracellular components of the transmembrane dystrophin–glycoprotein complex (DGC). The functional role of this complex in normal and pathological situations has not yet been clearly established. Dystrobrevin disappears from the muscle membrane in Duchenne muscular dystrophy (DMD), which results from dystrophin mutations, as well as in limb girdle muscular dystrophies (LGMD), which results from mutations affecting other members of the DGC complex.

6p system191 01-06-2013 34 5   Download

There is extensive evidence that FcR c-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR c-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR c-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin....

10p system191 01-06-2013 35 3   Download

Trypanosoma brucei is the cause of the diseases known as sleeping sickness in humans (T. brucei ssp. gambiense and ssp. rhodesiense) and ngana in domestic animals (T. brucei brucei) in Africa. Procyclic trypomastigotes, the tsetse vector stage, express a surface-bound trans-sialidase that transfers sialic acid to the glycosylphosphatidylinositol anchor of procyclin, a surface glycoprotein covering the parasite surface. Trans-sialidase is a unique enzyme expressed by a few trypanosomatids that allows them to scavenge sialic acid from sialylated compounds present in the infected host. ...

10p system191 01-06-2013 44 4   Download

P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. When the UIC2-shift was elicited by certain modulators (e.g. cyclosporin A or vinblastine, but not with verapamil or Tween 80), the subsequent binding of other monoclonal anti-Pgp Ig sharing epitopes with UIC2 (e.g. MM12.10) was abolished [Nagy, H., Goda, K., Arceci, R., Cianfriglia, M., ´ Mechetner, E. & Szabo Jr, G.

6p system191 01-06-2013 62 7   Download

In avian species, an egg envelope homologous to the mammalian zona pellucida is called the perivitelline membrane. We have previously reported that one of its components, a glycoprotein homologous to mammalian ZPC, is synthesized in the granulosa cells of the quail ovary. In the present study, we investigated the proteolytic cleavage of the newly synthesized ZPC and the secretion of ZPC from the granulosa cells. Western blot analysis of the cell lysates demonstrated that the 43-kDa protein is the precursor of mature ZPC (proZPC), and is converted to the 35-kDa protein before secretion. ...

9p research12 01-06-2013 36 4   Download

MFG-E8 (milk fat globule-EGF factor 8) is a peripheral membrane glycoprotein, which is expressed abundantly in lactating mammary glands and is secreted in association with fat globules. This protein consists of two-repeated EGF-like domains, a mucin-like domain and two-repeated discoidinlike domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain. To clarify the role of each domain on the peripheral association with the cell membrane, several domain-deletion mutants of MFG-E8 were expressed in COS-7 cells. ...

10p research12 01-06-2013 53 3   Download

Subunits (a, b and c) of the interleukin-2 receptor complex (IL-2R) are involved in both proliferative and activationinduced cell death (AICD) signaling of T cells. In addition, the signaling b and c chains are shared by other cytokines (e.g. IL-7, IL-9, IL-15). However, the molecular mechanisms responsible for recruiting/sorting the a chains to the signaling chains at the cell surface are not clear.

10p research12 01-06-2013 29 5   Download

In this study, we show that the G protein-coupled receptor agonist thrombin, the glycoprotein VI agonist convulxin, and the cytokine receptor Mpl agonist thrombopoietin (TPO) are able to induce activation of RAS in human platelets. Recruitment ofGRB2 by tyrosine-phosphorylated proteins in response to TPO and convulxin but not by thrombin occurred with a similar time-course to RAS acti-vation, consistent with a causal relationship.

7p research12 29-04-2013 40 3   Download

Mucin glycoproteins on breast cancer cells carry shortened carbohydrate chains. These partially deglycosylatedmucin 1 (MUC-1) structures are recognized by the monoclonal antibody SM3, which is being tested for its diagnostic utility. We used NMR spectroscopy to analyze the binding mode and thebindingepitopeof peptideandglycopeptideantigens to the SM3 antibody. The pentapeptide PDTRP and the glycopentapeptide PDT(O-a-D-GalNAc)RP are known lig-ands of the monoclonal antibody.

12p research12 29-04-2013 40 3   Download

The envelope protein gp64 of the baculovirusAutographa californicanuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein bothmediates pH-dependentmembrane fusionandbinds tohost cell receptors. Surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and powerful tool in molecular biology.

10p research12 23-04-2013 27 2   Download

The N-terminal fusion peptide of Sendai virus F1envelope glycoprotein is a stretchof 14aminoacids,most ofwhichare hydrophobic. Following this region, we detected a segment of 11 residues that are strikingly similar to the N-terminal fusion peptide. We found that, when anchored to the mem-brane by palmitoylation of its N-terminus, this segment (WT-palm-19–33) induces membrane fusion of large unila-mellar liposomes toalmost the same extent as a segment that includes the N-terminal fusion peptide....

9p research12 23-04-2013 40 2   Download

The zona pellucida, a transparent envelope surrounding the mammalian oocyte, consists of three glycoproteins, ZPA, ZPBandZPC, andplays a role in sperm–egg interactions. In bovines, these glycoproteins cannot be separated unless the acidic N-acetyllactosamine regions of the carbohydrate chains are removed by endo-b-Galactosidase digestion. Endo-b-Galactosidase-digestedZPBretains stronger sperm-binding activity than ZPC.

10p research12 23-04-2013 33 3   Download

The human multidrug resistance protein 2 (MRP2, symbol ABCC2) is a polytopic membrane glycoprotein of 1545 amino acids which exports anionic conjugates across the apical membrane of polarized cells. A chimeric protein composed of C-proximal MRP2 and N-proximal MRP1 localized to the apicalmembraneof polarizedMadin±Darby canine kidney cells (MDCKII) indicating involvement of the carboxy-proximal part of human MRP2 in apical sorting.

11p research12 23-04-2013 31 2   Download

Keyhole limpet (Megathura crenulata) hemocyanin (KLH), an extracellular respiratory protein, is widely used as hapten carrier and immune stimulant. Although it is generally accepted that the sugar constituents of this glycoprotein are likely to be implicated in the antigenicity and biomedical properties of KLH, knowledge of its carbohydrate structure is still limited. Therefore, we have investigated the N-linked oligosaccharides of KLH. Glycan chains were enzymati-cally liberated from tryptic glycopeptides, pyridylaminated and separated by two-dimensional HPLC....

15p tumor12 22-04-2013 33 2   Download

The thyrotropin receptor (TSHR) is a member of the G protein-coupled receptor superfamily. It has by now been clearly established that the maturation of the glycopro-teins synthesized in the endoplasmic reticulum involves interactions with molecular chaperones, which promote the folding and assembly of the glycoproteins. In this study, we investigated whether calnexin (CNX), calreti-culin (CRT) and BiP, three of the main molecular chap-erones present in the endoplasmic reticulum, interact with the TSHR and what effects these interactions might have on the folding of the receptor....

8p tumor12 22-04-2013 36 4   Download

aIIbb3, a member of the integrin family of adhesive protein receptors, is the most abundant glycoprotein on platelet plasma-membranes and binds to adhesive proteins via the recognition of short amino acid sequences, for example the ubiquitous RGD motif.However, elucidation of the ligand-binding domains of the receptor remains controversial, mainly owing to the fact that integrins are conformationally labile during purification and storage.

8p tumor12 20-04-2013 48 2   Download

Lactoferrin (LF) is a Fe 3+ -binding glycoprotein, first recognized in milk and then in other human epithelial secretions and barrier fluids. Many different functions have been attributed to LF, including protection from iron-induced lipid peroxidation, immunomodulation and cell growthregulation, DNA binding, and transcriptional acti-vation. Its physiological role is still unclear, but it has been suggested to be responsible for primary defense against microbial and viral infection.

9p tumor12 20-04-2013 36 3   Download