Glycosaminoglycan chain analysis

Domain IV, consisting of about 230 residues, represents a particular protein module so far found only in lamininb1 andb2 chains. Both domains were obtained by recombi-nant production in mammalian cells. They showed a globular structure, as expected from electron microscopic examination of laminins. Fragmentb1IV was obtained as a monomer and a disul®de-bonded dimer, and both were modi®ed to50% by a single chondroitin sulfate chain attached to Ser721 of an SGD consensus sequence.

12p research12 23-04-2013 42 2   Download

The importance of proteoglycans for secretionof proteolytic enzymes was studied in the murine macrophage cell line J774. Untreated or 4b-phorbol 12-myristate 13-acetate (PMA)-stimulated macrophages were treated with hexyl-b-D-thioxyloside to interfere with the attachment of glycosaminoglycan chains to their respective protein cores. Activation of the J774 macrophages with PMA resulted in increased secretion of trypsin-like serine proteinase activity.

10p tumor12 20-04-2013 34 3   Download