In eukaryotes. in this study

The 26S proteasome, a protein complex consisting of a 20S proteasome and a pair of 19S regulatory particles (RP), is involved in ATP-dependent proteolysis in eukaryotes. In yeast, theRP contains six different ATPase subunits and, at least, 11 non-ATPase subunits. In this study, we identi®ed the rice homologs of yeast RP subunit genes from the rice expressed sequence tag (EST) library.

10p research12 29-04-2013 38 3   Download

Protein histidine phosphorylation in eukaryotes has been sparsely studied compared to protein serine/threonine and tyrosine phosphorylation. In an attempt to rectify this by probing porcine liver cytosol with the phosphohistidine-containing peptide succinyl-Ala-His(P)-Pro-Phe-p-nitro-anilide (phosphopeptide I), we observed a phosphatase activity that was insensitive towards okadaic acid and EDTA.

8p tumor12 22-04-2013 36 2   Download

Previous work from this laboratory has shown that the spectral and functional properties of a prokaryotic xanthine dehydrogenase fromComamonas acidovoransshow some similarities to those of the well-characterized eukaryotic enzymes isolated from bovine milk and from chicken liver [Xiang, Q. & Edmondson, D.E. (1996) Biochemistry35, 5441–5450]. Therefore, this system was chosen to study the factors involved in the expression of functional recombinant enzyme in Escherichia colito provide insights into the assembly of the functional Mo-pyranopterin center....

11p fptmusic 12-04-2013 39 2   Download

Eukaryotic genomes are composed of isochores, i.e. long sequences relat-ively homogeneous in GC content. In this paper, the isochore structure of Arabidopsis thalianagenome has been studied using a windowless technique based on theZcurve method and intuitive curves are drawn for all the five chromosomes. Using these curves, we can calculate the GC content at any resolution, even at the base level.

9p fptmusic 11-04-2013 40 3   Download

Searching the genome sequence ofStreptococcus pneumoniaerevealed the presence of a single Ser⁄Thr protein kinase genestkP linked to protein phosphatasephpP. Biochemical studies performed with recombinant StkP suggest that this protein is a functional eukaryotic-type Ser⁄Thr protein kinase.In vitrokinase assays and Western blots ofS. pneumoniaesubcellu-lar fractions revealed that StkP is a membrane protein.

12p awards 06-04-2013 29 3   Download

In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA.

8p awards 05-04-2013 30 5   Download

Advanced glycation end-products (AGEs) of DNA are formed spontane-ously by the reaction of carbonyl compounds such as sugars, methylglyoxal or dihydroxyacetonein vitro andin vivo. Little is known, however, about the biological consequences of DNA AGEs. In this study, a method was developed to determine the parameters that promote DNA glycation in cultured cells.

12p media19 05-03-2013 27 3   Download

The purpose of this review is to discuss the effect of natural antioxidant compounds as modulators of the 20Sproteasome, a multi-enzymatic multi-catalytic complex present in the cytoplasm and nucleus of eukaryotic cells and involved in several cellular activities such as cell-cycle progression, pro-liferation and the degradation of oxidized and damaged proteins.

15p vinaphone15 28-02-2013 57 3   Download

Lysine-specific demethylase 1 (LSD1) is an enzyme that removes methyl groups from mono- and dimethylated Lys4 of histone H3, a post-transla-tional modification associated with gene activation. Human LSD1 was the first histone demethylase to be discovered and this enzymatic activity is conserved among eukaryotes.

9p vinaphone15 25-02-2013 30 2   Download

DNA methylation affects important developmental processes in both plants and animals. The process of methylation of cytosines at C-5 is catalysed by DNA methyltransferases (MTases), which are highly conserved, both struc-turally and functionally, in eukaryotes. In this study, we identified and characterized cytosine DNA MTase genes that are activated with the onset of reproductive development in rice.

11p viettel02 20-02-2013 44 5   Download

Peroxisomes are cell organelles that are present in almost all eukaryotic cells and involved in a large range of metabolic pathways. The organelles are highly dynamic in nature: their number and enzyme content is highly variable and continuously adapts to prevailing environmental conditions. This review summarizes recent relevant developments in research on pro-cesses that are involved in the regulation of peroxisome abundance and maintenance.

10p viettel02 19-02-2013 32 3   Download

Cell navigation is the process whereby cells or cytoplasmic extensions are guided from one point to another in multicellular organisms or, in the case of unicellular eukaryotic organisms, in the environment. Recent work has demonstrated that membrane trafficking plays an important role in this process.

9p cosis54 04-01-2013 51 4   Download