Lysozyme

Nhằm giúp các bạn đang học môn Sinh học đại cương có thêm tài liệu tham khảo, mời các bạn cùng tham khảo bài thuyết trình đề 3 "Đặc điểm, vai trò của các bào quan có cấu trúc màng lysozyme, vi thể, không bào" dưới đây. Nội dung bài thuyết trình giới thiệu đến các bạn những nội dung về lysozome, vi thể, không bào.

22p boo1202 18-09-2015 81 5   Download

Bài giảng Hóa sinh đại cương - Chương 3 Enzyme cung cấp cho người học những kiến thức như: Khái niệm chung về hiện tượng xúc tác; Enzyme là chất xúc tác sinh học; Đơn vị hoạt lực của enzyme; Phần lớn các enzyme có bản chất là protein; Trung tâm hoạt động của enzyme;...

80p buctranhdo 02-07-2021 85 11   Download

Mục tiêu của luận án là xác định giá trị chẩn đoán của từng xét nghiệm: Phosphatse kiềm dịch màng phổi (ALP DMP) và tỉ số ALP dịch màng phổi / huyết thanh (ALP DMP/HT), Lysozyme dịch màng phổi (Lys DMP) và tỉ số Lysozyme dịch màng phổi/ huyết thanh (Lys DMP/HT), Adenosine deaminase dịch màng phổi (ADA DMP) và Interferon gamma dịch màng phổi (INF-γ DMP) trong TDMP lao

27p cotithanh321 06-08-2019 35 6   Download

Mục tiêu của luận án là nhận xét về đặc điểm lâm sàng, cận lâm sàng của nhóm bệnh nhân được chẩn đoán cuối cùng là tràn dịch màng phổi do lao. Xác định giá trị chẩn đoán của từng xét nghiệm: Phosphatse kiềm dịch màng phổi (ALP DMP) và tỉ số ALP dịch màng phổi / huyết thanh (ALP DMP/HT), Lysozyme dịch màng phổi (Lys DMP) và tỉ số Lysozyme dịch màng phổi/ huyết thanh (Lys DMP/HT), Adenosine deaminase dịch màng phổi (ADA DMP) và Interferon gamma dịch màng phổi (INF-γ DMP) trong TDMP do lao.

181p cotithanh321 06-08-2019 44 2   Download

Reduced denatured lysozyme tends to aggregate at neutral pH and competition between productive folding and aggregation substantially reduces the efficiency of refolding. Trigger factor, a folding catalyst and chaperone can, depending on the concentration of trigger factor and the solution conditions, cause either a substantial increase (chaperoneactivity) or a substantial decrease (antichaperone activity) in the recoveryof native lysozymeas comparedwith spontaneous refolding.

8p research12 23-04-2013 40 3   Download

Antibacterial peptides were isolated fromhuman peripheral granulocytes of a healthy donor who had been treated with granulocyte-colony stimulating factor (G-CSF) and cortisol. Peptides were solubilized in acidi®ed chloroform/methanol, and partitioned in chloroform/methanol/water. Water-soluble polypeptides were separated by cation-exchange and reversed-phase chromatography. Several previously char-acterized antibacterial polypeptides were identi®ed; defen-sins 1±3, defensin 4, lysozyme, eosinophil cationic protein, and calgranulin A. ...

7p research12 23-04-2013 26 3   Download

One-electron oxidation of six different c-type lysozymes from hen egg white, turkey egg white, human milk, horse milk, camel stomach and tortoise was studied by gamma-and pulse-radiolysis.In the first step, one tryptophan side chain is oxidized to indolyl free radical, which is produced quantitatively.As shown already, the indolyl radical subse-quently oxidizes a tyrosine side chain to the phenoxy radical in an intramolecular reaction.However this reaction is not total and its stoichiometry depends on the protein.Rate constants also vary between proteins, from 120Æs )1 to 1000Æs )1 at pH 7.

7p tumor12 20-04-2013 41 2   Download

Proteins tend to form inactive aggregates at high tempera-tures. We show that polyamines, which have a relatively simple structure as oligoamids, effectively prevent thermal inactivation and aggregation of hen egg lysozyme. In the presence of additives, including arginine and guanidine (100 mM), more than 30% of 0.2 mgÆmL )1 lysozyme in sodium phosphate buffer (pH 6.5) formed insoluble aggre-gates by heat treatment (98
C for 30 min). 1

8p fptmusic 12-04-2013 44 3   Download

Arginine is a versatile additive to prevent protein aggrega-tion. This paper shows that arginine ethylester (ArgEE) prevents heat-induced inactivation and aggregation of hen egg lysozyme more effectively than arginine or guanidine. TheadditionofArgEEdecreased themelting temperatureof lysozyme. This data could be interpreted in terms of ArgEE binding to unfolded lysozyme, possibly through the ethyl-ated carboxyl group, which leads to effective prevention of intermolecular interaction among aggregation-prone mole-cules.

6p awards 05-04-2013 26 3   Download

On the basis of a partial N-terminal sequence, Jolle`s and Jolle`s [Jolle`s, J., & Jolle`s, P. (1975)Eur. J. Biochem.54, 19–23] previously proposed that the lysozyme from the starfishAsterias rubensrepresents a new form of lysozyme, called typei(invertebrate) lysozyme. Indeed, it differed from both the typesc(chicken) and g(goose) known in other animals, as well as from plant and phage lysozymes. Recently, several proteins belonging to the same family have been isolated from protostomes. Here we report the com-plete mature protein sequence and cDNA sequence of the lysozyme from Asterias. ...

6p dell39 03-04-2013 43 2   Download

We report the secreted expression byPichia pastorisof two human lyso-zyme variants F57I and W64R, associated with systemic amyloid disease, and describe their characterization by biophysical methods. Both variants have a substantially decreased thermostability compared with wild-type human lysozyme, a finding that suggests an explanation for their increased propensity to form fibrillar aggregates and generate disease.

10p dell39 27-03-2013 40 4   Download

While many antibodies with strong antigen-binding affinity have stable variable regions with a strong antibody heavy chain variable region frag-ment (VH)⁄antibody light chain variable region fragment (VL) interaction, the anti-lysozyme IgG HyHEL-10 has a fairly strong affinity, yet a very weak VH⁄VLinteraction strength, in the absence of antigen.

11p inspiron33 26-03-2013 43 5   Download

The interaction of antibodies (Abs) with protein antigens (Ags) of different size, such as hen egg white lysozyme, ovalbumin, and bovine serum albu-min, was examined using analytical ultracentrifugation, electrospray ioniza-tion time-of-flight mass spectrometry, and surface plasmon resonance in order to estimate regional and segmental Ab flexibility.

12p inspiron33 26-03-2013 41 4   Download

The role of the two disulfide bonds (Cys4–Cys60 and Cys18–Cys29) in the activity and stability of goose-type (G-type) lysozyme was investigated using ostrich egg-white lysozyme as a model. Each of the two disulfide bonds was deleted separately or simultaneously by substituting both Cys residues with either Ser or Ala.

13p media19 06-03-2013 33 3   Download

Amyloid aggregation of polypeptides is related to a growing number of pathologic states known as amyloid disorders. There is a great deal of interest in developing small molecule inhibitors of the amyloidogenic pro-cesses.

11p media19 05-03-2013 40 2   Download

We have reported previously that the calcium-depleted form of bovine a-lactalbumin (apoa-LA) interacts with hen egg-white lysozyme (LYS) to form spherical supramolecular structures. These supramolecular structures contain an equimolar ratio of the two proteins. We further explore here the organization of these structures.

9p media19 05-03-2013 41 1   Download

Protein oligomeric complexes have emerged as a major target of current research because of their key role in aggregation processes in living systems andin vitro. Hydrophobic and charged surfaces may favour the self-assembly process by recruiting proteins and modifying their interactions. We found that equine lysozyme assembles into multimeric complexes with oleic acid (ELOA) at the solid–liquid interface within an ion-exchange chromatography column preconditioned with oleic acid.

15p vinaphone15 25-02-2013 51 4   Download

In ruminants, some leaf-eating animals, and some insects, defensive lyso-zymes have been adapted to become digestive enzymes, in order to digest bacteria in the stomach. Digestive lysozyme has been reported to be resis-tant to protease and to have optimal activity at acidic pH. The structural basis of the adaptation providing persistence of lytic activity under severe gastric conditions remains unclear.

9p viettel02 22-02-2013 40 3   Download

Humana-lactalbumin made lethal to tumor cells (HAMLET) and equine lysozyme with oleic acid (ELOA) are complexes consisting of protein and fatty acid that exhibit cytotoxic activities, drastically differing from the activity of their respective proteinaceous compounds. Since the discovery of HAMLET in the 1990s, a wealth of information has been accumulated, illuminating the structural, functional and therapeutic properties of protein complexes with oleic acid, which is summarized in this review.

12p viettel02 19-02-2013 42 2   Download

Prediction and understanding of the folding and stability of the 3D struc-ture of proteins is still a challenge. The different atomic interactions, such as non polar contacts and hydrogen bonding, are known but their exact relative weights and roles when contributing to protein folding and stability are not identified.

17p cosis54 08-12-2012 43 2   Download