Methods of screening

The thesis is structured as follows: Chapter 1 reviews the literature and outlines the importance of blood, clinical use of blood, current methods of blood collection including alternative methods and the benefits of DBS. Chapter 2 summaries the overall methods used in the project. Chapter 3 explains the utility of hemaPEN® from both the collection and analytical perspective. Chapter 4 expands on the HCT bias associated with standard DBS method. Chapter 5 expands on the use of DBS in other fields such as genomic and epigenomics.

164p runthenight04 02-02-2023 6 2   Download

Target: Selecting a yeast strain having high activity of ethanol fermentation producing a specific aroma of fermented pineapple Queen juice, suitable for the production of comprehensive quality brandy from pineapple.

28p thebadguys 08-06-2021 22 3   Download

Two objectives: Establish the procedure for interlocking intramedullary nailing without opening the fracture site under the intensifying screen. Evaluate the results of treatment for femoral shaft fractures with the method of interlocking intramedullary nailing without opening the fracture site and compare to the method of interlocking nailing opening the fracture site.

30p petsematary 02-06-2021 16 4   Download

The primary objective of the current research was to evaluate the available chickpea germplasms by screening method against root knot nematode M. incognita.

8p chauchaungayxua11 23-03-2021 16 2   Download

Detection and screening of genes related to biosynthesis of substances with protease inhibitory activity from microorganisms associated with sponge collected from the Mien trung sea of Vietnam by metagenomics method. Then, selective gene, expression of in vitro and determination of activity of the expressed protein.

27p capheviahe26 25-01-2021 17 3   Download

In this study, the antimicrobial activity and phytochemical constituents of neem leaves and lemon grass oil extracts were evaluated. Oil extracts of neem leaves and lemon grass were obtained by solvent extraction method using hexane and ethanol.

8p lucastanguyen 01-06-2020 39 4   Download

A series of novel 10-phenylsulfonyl-2-substituted-4,10 dihydrobenzo[4,5]imidazo[1,2- a]pyrimidin-4-one derivatives obtained from N-sulfonation of 2-substituted-pyrimido[1,2- a]benzimidazol-4(10H)-ones and screened for for their in vitro anti-tuberculosis activities against Mycobacterium tuberculosis H37Rv by Microplate Alamar Blue Assay (MABA) method.

8p tocectocec 25-05-2020 14 1   Download

Antisense oligodeoxynucleotides (AS-ODNs) are frequently used for the down-regulation of protein expression.Because the majority of potential antisense sequences lacks effect-iveness, fast screening methods for the selection of effective AS-ODNs are needed.We describe a new cellular screening assay for the evaluationof the potency and specificityof new antisense sequences.

10p research12 29-04-2013 32 3   Download

The (ba)8-barrel is the most common enzyme fold and it is capable of cata-lyzing an enormous diversity of reactions. It follows that this scaffold should be an ideal starting point for engineering novel enzymes by directed evolution. However, experiments to date have utilized in vivo screens or selections and the compatibility of (ba)8-barrels with in vitro selection methods remains largely untested.

14p fptmusic 11-04-2013 41 3   Download

To facilitate the process of proteindesign and learn the basic rules that control the structure and stability of proteins, combinatorial methods have been developed to select or screen proteins with desired properties from libraries of mutants. One such method uses phage-display and proteo-lysis to select stably folded proteins. This method does not rely on specific properties of proteins for selection. There-fore, in principle it can be applied to any protein.

6p dell39 03-04-2013 31 4   Download

The aim of this study is to discover and characterize novel energy homeo-stasis-related molecules. We screened stock mouse embryonic stem cells established using the exchangeable gene trap method, and examined the effects of deficiency of the target gene on diet and genetic-induced obesity.

10p mobifone23 18-01-2013 27 3   Download

With a pen-based system, you use an electronic pen to write on the screen and choose commands. Pens are common input devices for handheld computers, like “personal digital assistants (PDAs).” Pens are handy for making notes or selecting commands, not for inputting a lot of text.

20p trada85 18-01-2013 40 5   Download

Weak and transient protein–protein interactions are associated with biolog-ical processes, but many are still undefined because of the difficulties in their identification. Here, we describe a redesigned method to screen transient protein–protein interactions by using a novel signal amplification circuit, which is incorporated into yeast to artificially magnify the signal responding to the interactions.

9p cosis54 04-01-2013 53 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A rapid method to screen putative mRNA targets of any known microRNA

8p dauphong1 21-12-2011 46 3   Download

Prevalence of severe mental distress and its correlates in a population-based study in rural south-west Uganda Eugene Kinyanda1*, Laban Waswa1, Kathy Baisley2 and Dermot Maher1,2 Abstract Background: The problem of severe mental distress (SMD) in sub-Saharan Africa is difficult to investigate given that a substantial proportion of patients with SMD never access formal health care. This study set out to investigate SMD and it’s associated factors in a rural population-based cohort in south-west Uganda.

9p thulanh29 17-12-2011 53 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Detection of DNA mismatch repair proteins in fresh human blood lymphocytes - towards a novel method for hereditary non-polyposis colorectal cancer (Lynch syndrome) screening

7p toshiba22 21-11-2011 62 3   Download

Parkinson’s disease is the second most common neurodegenerative disorder. The pathological hallmark of the disease is degeneration of midbrain dopaminergic neurons. Genetic association studies have linked 13 human chromosomal loci to Parkinson’s disease. Identification of gene(s), as part of the etiology of Parkinson’s disease, within the large number of genes residing in these loci can be achieved through several approaches, including screening methods, and considering appropriate criteria.

5p toshiba23 18-11-2011 57 4   Download

The highly pathogenic H5N1 is a major avian pathogen that crosses species barriers and seriously affects humans as well as some mammals. It mutates in an intensified manner and is considered a potential candidate for the possible next pandemic with all the catastrophic consequences. Methods: Nasal swabs were collected from donkeys suffered from respiratory distress. The virus was isolated from the pooled nasal swabs in specific pathogen free embryonated chicken eggs (SPF-ECE).

6p toshiba23 18-11-2011 52 4   Download

Schizophrenia is a complex disorder with involvement of multiple genes. Methods: In this study, genome-wide screening for DNA copy-number variations (CNVs) was conducted for ten pairs, a total of 20 cases, of affected siblings using oligonucleotide array-based CGH. Results: We found negative symptoms were significantly more severe (p

9p toshiba23 18-11-2011 66 6   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Development of a method for screening short-lived proteins using green fluorescent protein...

8p thulanh19 09-11-2011 53 4   Download