Rna isolation

Molecular knowledge regarding the primary esophageal achalasia is essential for the early diagnosis and treatment of this neurodegenerative motility disorder. Therefore, there is a need to find the main microRNAs (miRNAs) contributing to the mechanisms of achalasia. This study was conducted to determine some patterns of deregulated miRNAs in achalasia. This case-control study was performed on 52 patients with achalasia and 50 nonachalasia controls. The miRNA expression profiling was conducted on the esophageal tissue samples using the next-generation sequencing (NGS).

13p thiencuuchu 27-11-2021 9 2   Download

The aim of the study is to characterize D.nodosus isolates with respect to virulence. In the present investigation gelatin test and elastase test were used to study the virulence of D.nodosus isolates recovered in the study.

13p nguaconbaynhay7 15-08-2020 18 1   Download

Nghiên cứu kháng thể thụ động ở vịt con 1 ngày tuổi, các đối tượng không tiêm phòng gồm ngỗng, ngan, vịt và gà thả vườn cũng như sự lưu hành của virus H5N1 được khảo sát ở Đồng Tháp và Hậu Giang. Hiệu giá kháng thể HI được xác định bằng phương pháp ức chế ngưng kết hồng cầu HI. Sự hiện diện của virus H5N1 xác định bằng kỹ thuật RT-PCR với bộ kit Mag MAXTM - 96AI/ND viral RNA isolate kit (USA). Kết quả cho thấy: Vịt con giống Super M 1 ngày tuổi nhận được kháng thể thụ động kháng lại virus...

7p sunshine_9 23-07-2013 78 6   Download

The plastid transcription kinase (PTK), a component of the major RNA polymerase complex from mustard chloroplasts, has been implicated in redox-mediated regulation of plastid gene expression. A cloning strategy to define the PTK gene(s) resulted in the isolation of a full-length cDNA for a protein with overall high homology with the a subunit of cytosolic casein kinase (CK2) that contained an N-terminal extension for a putative plastid transit peptide.

9p system191 01-06-2013 45 4   Download

RNA aptamers with anity for the Ras-binding domain (RBD) of Raf-1 were isolated from a degenerate pool by in vitroselection.These aptamers eciently inhibited theRas interaction with the Raf-1 RBD, and also inhibited Ras-induced Raf-1 activation in a cell-free system. The RNA aptamer with the most potent inhibitory e€ect speci®cally inhibited the RasáRaf-1 interaction and had no anity for the RBD of the RGL protein, a homolog of the Ral GDP dissociation stimulator.

8p research12 23-04-2013 37 2   Download

Cold shock proteins (CSPs) form a family of highly conserved bacterial proteins capable of single-stranded nucleic acid binding. They are suggested to act as RNA chaperones during cold shock inhibiting the formation of RNA secondary structures, which are unfavourable for transcription and translation.

12p fptmusic 12-04-2013 37 2   Download

GE23077, a novel microbial metabolite recently isolated from Actinomadurasp. culture media, is a potent and selective inhibitor of bacterial RNApolymerase (RNAP). It inhibitsGram-positive (Bacillus subtilis) andGram-negative (Escherichia coli)RNAPs with IC50 values (i.e. the concen-tration at which the enzyme activity is inhibited by 50%) in the 10 )8 Mrange, whereas it is not active onE. coliDNA polymerase or on eukaryotic (wheat germ) RNAP II (IC50 values10 )4 Min both cases).

0p awards 05-04-2013 55 2   Download

RNA polymerase II (RNAPII) requires a set of general transcription factors )TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH)to initiate transcription from a gene promoterin vitro. General transcription factors have been isolated from Saccharomyces cerevisiae,rat, human and Drosophila,and their corresponding cDNAs have been cloned. In this report, we describe a reconstitutedin vitro transcription system that consists of the following prepara-tions of factors from the yeastSchizosaccharomyces pombe: affinity-purified RNAPII, TFIIH, and recombinant TBP, TFIIB, TFIIE and TFIIF. ...

12p dell39 03-04-2013 60 3   Download

Bcp2 was identified as a putative peroxiredoxin (Prx) in the genome data-base of the aerobic hyperthermophilic archaeonSulfolobus solfataricus.Its role in oxidative stress was investigated by transcriptional analysis of RNA isolated from cultures that had been stressed with various oxidant agents.

11p dell39 27-03-2013 35 4   Download

The crustacean hyperglycemic hormone (CHH) plays an important role in the regulation of hemolymph glucose levels, but it is also involved in other functions such as growth, molting and reproduction. In the present study we describe the first CHH family gene isolated from the Atlantic Ocean shrimp Litopenaeus schmitti.

9p inspiron33 23-03-2013 43 4   Download

Recent studies suggest that diverse types of intronic and small non-coding RNAs play pivotal regulatory roles, often as a part of ribonucleoprotein (RNP) complexes, in a variety of cellular proces-ses such as transcription and translation. Thus, isolation and char-acterization of novel RNP complexes, such as micro RNPs, small nuclear RNPs, small nucleolar RNPs, and heteronuclear RNPs, are crucial to understand the biological systems.

20p galaxyss3 19-03-2013 44 2   Download

At the optimal temperature (65C),Thermus thermophiluspolynucleotide phosphorylase (Tth PNPase), produced inEscherichia colicells and isolated to functional homogeneity, completely destroys RNAs that possess even a very stable intramolecular secondary structure, but leaves intact RNAs whose 3¢ end is protected by chemical modification or by hybridization with a complementary oligonucleotide.

13p media19 06-03-2013 43 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development

11p panasonic06 26-12-2011 55 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Rapid isolation of mycoviral double-stranded RNA from Botrytis cinerea and Saccharomyces cerevisiae

7p dauphong1 21-12-2011 38 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Meiosis-specific gene discovery in plants: RNA-Seq applied to isolated Arabidopsis male meiocytes

14p panasonic02 14-12-2011 52 5   Download

Rifampin Bacteria rapidly become resistant to rifampin by developing mutations in the B subunit of RNA polymerase that render the enzyme unable to bind the antibiotic. The rapid selection of resistant mutants is the major limitation to the use of this antibiotic against otherwise-susceptible staphylococci and requires that the drug be used in combination with another antistaphylococcal agent. Linezolid Enterococci, streptococci, and staphylococci become resistant to linezolid in vitro by mutation of the 23S rRNA binding site. Clinical isolates of E. faecium and E.

5p colgate_colgate 21-12-2010 69 3   Download

Other Nondiphtherial Corynebacteria C. xerosis is a human commensal found in the conjunctiva, nasopharynx, and skin. This nontoxigenic organism is occasionally identified as a source of invasive infection in immunocompromised or postoperative patients and prosthetic joint recipients. C. striatum is found in the anterior nares and on the skin, face, and upper torso of normal individuals. Also nontoxigenic, this organism has been associated with invasive opportunistic infections in severely ill or immunocompromised patients. C.

8p colgate_colgate 21-12-2010 80 5   Download