Scientific papers

The research subject is the process of economic and mathematical modelling of time series characterizing the bitcoin exchange rate volatility, based on the use of artificial neural networks. The purpose of the work is to search and scientifically substantiate the tools and mechanisms for developing prognostic estimates of the crypto currency market development. The paper considers the task of financial time series trend forecasting using the LSTM neural network for supply chain strategies.

5p longtimenosee09 08-04-2024 10 1   Download

This thesis is presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy at the College of Business, RMIT University. It includes five scientific papers which have been submitted and are currently under review or accepted for publication in targeted journals. These papers comprise one conceptual paper and four empirical papers.

259p runthenight04 02-02-2023 7 3   Download

This paper will attempt to examine whether Organisational Psychology is a science and the extent to which its findings are of practical use to the managers. As it will be seen, the answer to the second half of this question depends on the answer given to the first one.

7p guineverehuynh 20-06-2020 12 2   Download

The paper revisits the various schools of thought of arts and science to critically analyse scientific realism, logical positivism, historic relativism and logical empiricism.

6p guineverehuynh 21-06-2020 13 1   Download

This paper pointed out achievements, constraints and the need of provision of appropriate intervention measures to overcome cumbersome in the current financial regime for scientific research in universities. Especially, the author proposed groups of solution with a view to improving the financing mechanism for S&T in universities, in general and in the Academy of Finance(AOF), in particular.

11p jangni6 07-05-2018 31 3   Download

Shikimate kinase was chosen as a convenient representative example of the subclass of a/b proteins with which to examine the mechanism of protein folding. In this paper we report on the refolding of the enzyme after denaturation in urea. As shown by the changes in secondary and tertiary structure monitored by far UV circular dichroism (CD) and fluorescence, respectively, the enzyme was fully unfolded in 4 M urea. From an analysis of the unfolding curve in terms of the two-state model, the stability of the folded state could be estimated as 17 kJÆmol)1.

9p system191 01-06-2013 46 5   Download

Certain amino acids, like glutamine and leucine, induce an anabolic response in liver. They activate p70 ribosomal protein S6 kinase (p70S6K) and acetyl-CoA carboxylase (ACC) involved in protein and fatty acids synthesis, respectively. In contrast, the AMP-activated protein kinase (AMPK), which senses the energy state of the cell and becomes activated under metabolic stress, inactivates by phosphorylation key enzymes in biosynthetic pathways thereby conserving ATP.

9p system191 01-06-2013 50 5   Download

A series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of Mycobacterium smegmatis and purified by gel-filtration and paper chromatography and TLC. Their structures were determined by HPLC and GLC to determine sugar composition and ratios, MALDI-TOF MS to measure molecular mass, methylation analysis to determine linkages, 1H-NMR to obtain anomeric configurations of glycosidic linkages, and exoglycosidase digestions followed by TLC to determine sequences and anomeric configurations of the monosaccharides....

8p system191 01-06-2013 36 5   Download

Penaeidins are a family of antimicrobial peptides constitutively produced and stored in the haemocytes of penaeid shrimp. In response to microbial stimulation, they are released into the blood circulation and they further attach to shrimp cuticle surfaces through a chitin-binding property. In the present paper, we have analysed their expression, regulation and distribution in shrimp tissues in response to experimental microbial challenge.

12p system191 01-06-2013 40 6   Download

Glyoxal is a key compound involved in glyoxal oxidase (GLOX)-dependent production of glyoxylate, oxalate and H2O2 by lignin-degrading basidiomycetes. In this paper, we report that glyoxal was produced from a metabolite of ligninolytic fungi, linoleic acid, by manganese peroxidase (MnP)-dependent lipid peroxidation. In the absence of the parent substrate of linoleic acid, the dialdehyde was oxidized by MnP and Mn(III) chelate to start free radical reactions with emission of chemiluminescence at 700– 710 nm. ...

9p system191 01-06-2013 69 7   Download

A cytosolic thyroid-hormone-binding protein (xCTBP), predominantly responsible for the major binding activity of T3 in the cytosol of Xenopus liver, has been shown to be identical to aldehyde dehydrogenase class 1 (ALDH1) [Yamauchi, K., Nakajima, J., Hayashi, H., Horiuchi, R. & Tata, J.R. (1999) J. Biol. Chem. 274, 8460–8469]. Within this paper we surveyed which signaling, and other, compounds affect the thyroid hormone binding activity and aldehyde dehydrogenase activity of recombinant Xenopus ALDH1 (xCTBP/xALDH1) while examining the relationship between these two activities....

8p research12 01-06-2013 38 4   Download

In this paper we address the problem of extracting features relevant for predicting protein±protein interaction sites from the three-dimensional structures of protein complexes. Our approach is based on information about evolutionary con-servation and surface disposition. We implement a neural network based system, which uses a cross validation proce-dure and allows the correct detection of 73% of the residues involved in protein interactions in a selected database comprising 226 heterodimers.

6p research12 29-04-2013 31 3   Download

This paper describes violaxanthin de-epoxidation in model lipid bilayers. Unilamellar egg yolk phosphatidylcholine (PtdCho) vesicles supplemented with monogalactosyldi-acylglycerol were found to be a suitable system for studying this reaction. Such a system resembles more the native thylakoid membrane and offers better possibilities for studying kinetics and factors controlling de-epoxidation of violaxanthin than a systemcomposed only of monogalacto-syldiacylglycerol and is commonly used in xanthophyll cycle studies. ...

10p research12 23-04-2013 30 3   Download

This paper deals with the purification and the partial char-acterization of glutathione S-transferase (GST) isoforms from the clam Ruditapes decussatus. For the first step of purification, two affinity columns, reduced glutathione (GSH)–agarose and S-hexyl GSH–agarose, were mounted in series. Four affinity fractionswere thus recovered. Further purification was performed using anion exchange chroma-tography. Seven fractions,whichpresent aGSTactivitywith 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, were collected and analyzed by RP-HPLC....

8p research12 23-04-2013 39 2   Download

A new approach of predicting structural classes of protein domain sequences is presented in this paper. Besides the amino acid composition, the composition of several dipeptides, tripeptides, tetrapeptides, pentapeptides and hexapeptides are taken into account based on the step-wise discriminant analysis. The result of jackknife test shows that this new approach can lead to higher pre-dictive sensitivityand specificityfor reduced sequence similaritydatasets.

7p research12 23-04-2013 33 3   Download

Achromobacterprotease I (API) has a unique region of aromatic ring stacking with Trp169–His210in close proxi-mity to the catalytic triad. This paper reveals the electrostatic role of aromatic stacking in the shift in optimum pH to the alkaline region, which is the highest pH range (8.5–10) among chymotrypsin-type serine proteases. The pH-activity profile of API showed a sigmoidal distribution that appears at pH 8–10, with a shoulder at pH 6–8.

7p research12 23-04-2013 41 2   Download

Structurally tachykinin-related peptides have been isolated fromvarious invertebrate species and shown to exhibit their biological activities through a G-protein-coupled receptor (GPCR) for a tachykinin-related peptide. In this paper, we report the identification of a novel tachykinin-related pep-tide receptor, the urechistachykinin receptor (UTKR) from the echiuroidworm,Urechis unitinctus.

9p research12 23-04-2013 24 2   Download

The serotype a-specific polysaccharide antigen ofActinoba-cillus actinomycetemcomitansis an unusual sugar, 6-deoxy-D-talose.Guanosinediphosphate (GDP)-6-deoxy-D-talose is the activated sugar nucleotide form of 6-deoxy-D-talose, which has been identified as a constituent of only a few microbial polysaccharides. In this paper, we identify two genes encoding GDP-6-deoxy-D-talose synthetic enzymes, GDP-a-D-mannose 4,6-dehydratase and GDP-4-keto-6-deoxy-D-mannose reductase, in the gene cluster required for the biosynthesis of serotype a-specific polysaccharide anti-gen from A.

9p tumor12 22-04-2013 31 2   Download

This paper describes the cloning of the genes coding for each component of the complex of toluene/o-xylene monooxy-genase fromPseudomonas stutzeriOX1, their expression, purification and characterization. Moreover, the reconsti-tution of the active complex from the recombinant subunits has been obtained, and the functional role of each compo-nent in the electron transfer from the electron donor to molecular oxygen has been determined.

11p tumor12 22-04-2013 44 3   Download

In the accompanying paper [Adams, H., Scotti, P.A., de Cock, H., Luirink, J. & Tommassen, J. (2002)Eur. J. Bio-chem.269, 5564–5571.], we showed that the precursor of outer-membrane proteinPhoEofEscherichia coliwithaGly to Leu substitution at position )10 in the signal sequence (G-10L) is targeted to the SecYEGtranslocon via the signal-recognition particle (SRP) route, instead of via the SecB pathway. Here, we studied the fate of the mutant precursor in aprlA4mutant strain.

9p tumor12 22-04-2013 39 3   Download