Temperature stability

The main aims of this research are: To determine the phase inversion temperatures for the formation of oil-in-brine nanoemulsions at various salt concentrations using conductivity measurements; To prepare the nano-emulsions at PIT points, and study their stability by measuring their droplet size, polydispersity and size distribution using dynamic light scattering technique; To confirm the DLS results using Cryo-SEM technique;...

324p runthenight07 01-03-2023 8 4   Download

This study focuses on the stability of nano-emulsions produced by phase inversion temperature method using n-Decane and n-Dodecane as the dispersed phase and pure water/brine as the aqueous phase (continuous phase). A non-ionic surfactant (Brij 30) was used in the preparation of the nano-emulsions. Three different types salt namely \(NaCl\), \(KCl\) and \(MgC{l_2}\) were used at different concentrations.

173p runthenight07 01-03-2023 7 3   Download

In this Masters thesis computational modelling techniques were employed to investigate iron-free apo-Lactoferrin (apo-Lf) structural conformation changes in the presence of variant temperature and pH. These conditions represent the environment most milk protein goes through in food processing and the production of food products.

162p runthenight04 02-02-2023 9 3   Download

The catalyst 7.5% NiO/TiO2 and seven samples of NiO/γ-Al2O3 with NiO content from 7.5% to 60% have been obtained and studied. Physico-chemical characteristics of the catalysts were determined by methods of Adsorption (BET), X-ray Diffraction (XRD), TemperatureProgrammed Reduction (TPR) and Hydrogen Pulse Titration. The catalytic properties of the obtained samples were investigated in the reaction of CO methanation at temperatures 180 – 220◦C and mole ratios hydrogen/carbon monoxide 25-100.

16p 12120609 01-06-2020 9 1   Download

MYRRHA is a multi-purpose research reactor able to operate in sub-critical and critical modes and currently in the design phase at SCK•CEN. The choice of LBE was driven by its chemical stability, low melting temperature, high boiling point, low chemical reactivity with water and air and a good neutronic performance.

9p christabelhuynh 29-05-2020 18 1   Download

Growth of the volumes of dangerous and critical welded metal structures constructed under low temperatures imposes additional requirements on the mechanical properties of the weld joints, particularly, on the impact resistance of the weld joint and on the stability of its values.

8p tohitohi 19-05-2020 12 0   Download

In this summary, we briefly review the recent studies focused on the structure and mechanical behavior of HEAs, covering the important issues from phase stability to elastic modulus, mechanical strength, hardness and fatigue resistance. Finally, we highlight a few key findings recently reported for HEAs and discuss the outstanding issues yet to be resolved.

20p tohitohi 19-05-2020 10 2   Download

(BQ) In the study, temperature measurements have been made for Ti–6Al–4V workpieces with various duty factors to clarify the essential causes of difficulty in machining titanium alloys and observe the optimal duty factor in terms of productivity and quality.

7p xuanphuongdhts 27-03-2017 50 4   Download

The thermal stabilityofperoxidase fromleavesof theAfrican oil palm treeElaeis guineensis(AOPTP) at pH 3.0 was studied by differential scanning calorimetry (DSC), intrinsic fluorescence, CD and enzymatic assays.The spectral parameters asmonitoredby ellipticity changes in the far-UV CD spectrum of the enzyme as well as the increase in tryp-tophan intensity emission upon heating, together with changes in enzymatic activity with temperature were seen to be good complements to the highly sensitive but integral methodofDSC....

7p research12 29-04-2013 45 3   Download

The crystal structure of citrate synthase from the thermo-philic ArchaeonSulfolobus solfataricus(optimum growth temperature ¼85
C) has been determined, extending the number of crystal structures of citrate synthase from differ-ent organisms to a total of five that span the temperature range over which life exists (from psychrophile to hyper-thermophile). Detailed structural analysis has revealed possible molecular mechanisms that determine the different stabilities of thefiveproteins.

11p tumor12 22-04-2013 62 3   Download

Neuroglobin (Ngb) and cytoglobin (Cygb), recent additions to the globin family, display a hexa-coordinated (bis-histidyl) heme in the absence of external ligands. Although these proteins have the classical globin fold they reveal a very high thermal stability with a melting temperature (Tm)of 100
C for Ngb and 95
C for Cygb. Moreover, flash photolysis experi-ments at high temperatures reveal that Ngb remains functional at 90
C

9p awards 06-04-2013 32 3   Download

FKBP22 from a psychrotrophic bacteriumShewanellasp. SIB1, is a dimer-ic protein with peptidyl prolyl cis-transisomerase (PPIase) activity. Accord-ing to homology modeling, it consists of an N-terminal domain, which is involved in dimerization of the protein, and a C-terminal catalytic domain. A longa3 helix spans these domains. An N-domain with the entirea3 helix (N-domain + ) and a C-domain with the entire a3 helix (C-domain + ) were overproduced inEscherichia coliin a His-tagged form, purified, and their biochemical properties were compared with those of the intact protein.

0p awards 05-04-2013 27 2   Download

The information required for successful assembly of an icosahedral virus is encoded in the native conformation of the capsidproteinand in its interactionwith the nucleic acid. Here we investigated how the packing and stability of virus capsids are sensitive to single amino acid substitutions in the coat protein. Tryptophan fluorescence, bis-8-anilinonaph-thalene-1-sulfonate fluorescence, CD and light scattering were employed to measure urea- and pressure-induced effects on MS2 bacteriophage and temperature sensitive mutants. ...

11p dell39 03-04-2013 38 4   Download

The functional activities of proteins are closely related to their molecular structure and understanding their structure–function relationships remains one of the intriguing problems of molecular biology. We investigated struc-tural changes in 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus(17b-HSDcl) induced by pH, temperature, salt, urea, guanidine hydrochloride, and coenzyme NADPH binding.

11p inspiron33 25-03-2013 43 4   Download

DNA-binding proteins from starved cells (Dps proteins) protect bacteria primarily from oxidative damage. They are composed of 12 identical subunits assembled with 23-symmetry to form a compact cage-like struc-ture known to be stable at temperatures 70C and over a wide pH range. Thermosynechococcus elongatus Dps thermostability is increased dramatically relative to mesophilic Dps proteins.

16p inspiron33 23-03-2013 47 4   Download

Life grows almost everywhere on earth, including in extreme environments and under harsh conditions. Organisms adapted to high temperatures are called thermophiles (growth temperature 45–75C) and hyperthermophiles (growth temperature ‡80C). Proteins from such organisms usually show extreme thermal stability, despite having folded structures very similar to their mesostable counterparts.

11p media19 04-03-2013 52 3   Download

Current theories agree that there appears to be no unique feature responsi-ble for the remarkable heat stability properties of hyperthermostable pro-teins. A concerted action of structural, dynamic and other physicochemical attributes are utilized to ensure the delicate balance between stability and functionality of proteins at high temperatures.

13p media19 04-03-2013 30 2   Download

In order to evaluate the role of 3(S)-hydroxyproline [3(S)-Hyp] in the triple-helical structure, we produced a series of model peptides with nine tripeptide units including 0–9 3(S)-hydroxyproline residues. The sequences are H-(Gly-Pro-4(R)Hyp) l -(Gly-3(S)Hyp-4(R)Hyp)m-(Gly-Pro-4(R)Hyp)n -OH, where (l, m, n) = (9, 0, 0), (4, 1, 4), (3, 2, 4), (3, 3, 3), (1, 7, 1) and (0, 9, 0). All peptides showed triple-helical CD spectra at room temperature and thermal transition curves.

11p vinaphone15 28-02-2013 31 3   Download

The hypothesis is tested that Jun–Fos activator protein-1 coiled coil inter-actions are dominated during late folding events by the formation of intri-cate intermolecular electrostatic contacts. A previously derived cJun–FosW was used as a template as it is a highly stable relative of the wild-type cJun–cFos coiled coil protein (thermal melting temperature = 63C versus 16C), allowing kinetic folding data to be readily extracted.

14p vinaphone15 27-02-2013 42 4   Download

The stability ofb-glucosidase from the hyperthermophile Pyrococcus furio-suswas studied as a function of pressure, temperature and pH. The confor-mational stability was monitored using FTIR spectroscopy, and the functional enzyme stability was monitored by inactivation studies.

9p vinaphone15 27-02-2013 28 2   Download