Báo cáo khoa học: Activation of transiently transfected reporter genes in 3T3 Swiss cells by the inducers of differentiation/apoptosis – dimethylsulfoxide, hexamethylene bisacetamide and trichostatin A
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Despitedecadesof investigation, theprimary siteof actionof the prototypical inducers of differentiation, dimethylsulfox-ide andhexamethylene bisacetamide (HMBA), has not been delineated.During studies designed to analyzecis-acting elements responsible for induction of stage-specific globin genes, we discovered the capacity of HMBA and dimethyl-sulfoxide toenhance the expressionof transiently transfected reporter genes derived from globin and nonglobin gene promoters, prominently innonerythroid3T3Swiss cells....
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