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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional
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The AnCOB group I intron fromAspergillus nidulans encodes a homing DNA endonuclease called I-AniI which also functions as a maturase, assisting in AnCOB intron RNA splicing. In this investigation we biochemically char-acterized the endonuclease activity of I-AniIin vitro and utilizedcompetitionassays toprobe the relationshipbetween theRNA- andDNA-binding sites.
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