Báo cáo khoa học: Tet repressor residues indirectly recognizing anhydrotetracycline
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Two tetracycline repressor (TetR) sequence variants sharing 63%identical aminoacidswere investigated in terms of their recognition specificity for tetracycline and anhydrotetra-cycline. Thermodynamic complexstabilities determined by urea-dependent unfolding reveal that tetracycline stabilizes bothvariants toa similar extentbut that anhydrotetracycline discriminates between them significantly. Isofunctional TetR hybrid proteins of these sequence variants were constructed and their denaturation profiles identified resi-dues 57 and 61 as the complexstability determinant....
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