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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:8

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Yeast Erv1p is a ubiquitous FAD-dependent sulfhydryl oxidase, located in the intermembrane space of mito-chondria.The dimeric enzyme is essential for survival of the cell.Besides the redox-active CXXC motif close to the FAD, Erv1p harbours two additional cysteine pairs.Site-directed mutagenesis has identified all three cysteine pairs as essential for normal function.The C-terminal cysteine pair is of structural importance as it contributes to the correct arrangement of the FAD-binding fold.Variations in dimer formation and unique colour changes of mutant proteins argue in favour of an interaction between the N-terminal cysteine pair with the redox centre of the partner monomer....

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Nội dung Text: Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre

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