Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro
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A number of antibiotics have been reported to disturb the decoding process in prokaryotic translation and to inhibit the function of various natural ribozymes. We investigated the eect of several antibiotics on in vitro splicing of a eukaryotic nuclear pre-mRNA (b-globin). Of the eight antibiotics studied, erythromycin, Cl-tetracycline and streptomycinwere identi®ed as splicing inhibitors in nuclear HeLa cell extract. TheKi values were 160, 180 and 230lM, respectively.
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