Agar well diffusion

This study aims to provide an in vitro evidence for the potential antibacterial activity of the ethanolic and aqueous extracts from fruiting bodies of the Pycnoporus sanguineus (L.: Fr.) Murrill mushroom via agar well diffusion method (concentration of extracts 250 mg/mL) and determination of the antibacterial activity and minimum inhibitory concentrations (MIC) of ethanolic and aqueous extracts from the fruiting bodies of this mushroom by agar dilution method (concentration range of 25; 12.5; 6.25; 3.125 and 1.5625 mg/mL).

8p viporsche 28-10-2022 6 2   Download

The objective of this study was to evaluate the toxicity assessment of Pseudomonas silesiensis strain A3 CuNPs against human normal (Wi38) and cancer (A549) cell lines as well as antimicrobial activity agent.

8p schindler 30-12-2021 8 0   Download

The aim of this study was to evaluate the antimicrobial activity of three commercial GSE (Grapefruit Seed Extract) products against six bacterial and one fungal strain, through the agar well diffusion method, determination of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC). Obtained results indicate strong antimicrobial effects of investigated extracts, with different microbial susceptibility, including the multidrug-resistant (MDR) bacteria.

4p mudbound 10-12-2021 11 1   Download

A series of new extensively conjugated E -stilbene azomethines (5a–5h) were synthesized and screened for their antioxidant and antimicrobial activity. The compounds were tested against bacterial (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus subtilis) and fungal strains (Aspergillus niger, A. flavus, and Trichoderma harzianum) using the agar well diffusion method.

16p tudichquannguyet 29-11-2021 17 1   Download

Lá Neem (xoan chịu hạn) Azadirachta indica A.Juss Meliaceae được chiết siêu âm với các dung môi có độ cồn khác nhau, lọc, cô dịch chiết đến cao đặc, tiến hành đánh giá hoạt tính kháng khuẩn của cao đặc bằng phương pháp khuếch tán qua giếng thạch (well diffusion agar). Từ kết quả hiệu suất chiết và hoạt tính kháng khuẩn, xây dựng quy trình sản xuất cao khô lá xoan thu mua tại Ninh Thuận với dung môi cồn 25% có hoạt tính kháng khuẩn với vi khuẩn gram dương Staphylococcus aureus (MIC=80 mg/ml).

8p vibigates 31-10-2021 44 2   Download

Nghiên cứu này được thực hiện nhằm khảo sát hoạt tính kháng khuẩn và khả năng kháng ôxy hóa của lá Đinh lăng Polyscias fruticosa (L.) Harms. Tiềm năng điều trị bệnh tiêu chảy của lá Đinh lăng sẽ được khảo sát thông qua phương pháp khuếch tán trên giếng thạch (agar well diffusion method) dựa trên phương pháp của Anonymous (1996) và Hadecek, ctv (2000). Mời các bạn cùng tham khảo!

9p limingao 29-06-2021 60 2   Download

The present investigation was undertaken to isolate, characterize and screen the actinomycetes from salt pan sediment soils.

13p trinhthamhodang11 27-04-2021 11 2   Download

The metabolite 2-(1,1-diallyl-but-3-enyl)-5-nonyl-phenol isolated from Streptomyces sp. TC1 exhibited antimicrobial activity against bacterial leaf blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). The in vitro antimicrobial activity was found to be between 1.8-2.5 cm in agar well diffusion assay against the tested Xoo isolates and 200 µg/ml in terms of minimum inhibitory concentration. The isolated compound showed well binding with the virulence proteins of Xoo by producing a better docking score in molecular docking studies.

10p nguaconbaynhay10 22-01-2021 12 1   Download

The study focused on determining the antibacterial properties of P. nigrum against pathogenic bacteria and analyzing the phytochemical constituents responsible for these medicinal activities. The plant seeds were collected, shade dried and powdered and subjected to extraction by Cold maceration in various solvents.

10p nguaconbaynhay8 13-10-2020 11 1   Download

Oral diseases are among the major public health problems and the most common of chronic diseases that affect mankind. Essential oils could serve as an important natural alternative to prevent microbial growth in oral infection diseases. This study was undertaken to determine the in vitro anticariogenic activities of 11 essential oils against dental pathogenic bacteria (Staphylococcus aureus, Streptococcus mutans and Streptococcus pyogenes) and fungi (Candida albicans and Candida parapsilosis) using agar well diffusion method, followed by determination of MIC.

14p trinhthamhodang1215 23-09-2020 11 1   Download

The fruit (Nabag or Christ's Thorn Jujube) of Sidir tree (Ziziphus spina-christi) is a native snack food in Sudan. The antibacterial properties of methanol fruit extract of the Sudanese Ziziphus spina-christi was investigated against different ATCC strains of Gram-positive and Gram-negative bacteria.

7p trinhthamhodang7 31-08-2020 8 1   Download

The study measured and compared quality parameters such as pH, moisture content, ash content and hydrogen peroxide concentration of three types of honey from different localities in Ghana. Using the agar well diffusion, antimicrobial potential of natural honey from two different sources as well as an adulterated honey were determined against Staphylococcus aureus (ATCC 25923), Escherichia coli(ATCC 35218)and Klebsiella pneumonia (ATCC 27736). The results of the physico-chemical properties showed the following range of values; pH of (3.68– 4.49), moisture content (17.20 – 22.

11p caygaocaolon6 30-07-2020 10 1   Download

Antifungal activities of the three mushrooms Pleurotus pulmonarius, Pleurotus florida and Schizophyllum commune ethanol extracts were evaluated against some plant pathogenic fungi (Aspergillus flavus, Aspergillus niger and Alternaria solani, Colletotrichum graminicola, Fusarium oxysporum) by using agar well diffusion method. Minimum inhibitory concentration (MIC) was investigated for 1-20 mg/ml concentration to find out the lowest concentration of the sample that inhibits the growth of the test organisms.

8p angicungduoc6 22-07-2020 15 1   Download

The present study was conducted to assess the antimicrobial potential and phytochemical analysis of Aloe vera (Aloe barbadensis Miller) leaves extracts.The extracts were prepared by the sequential cold maceration method by using hexane, ethyl acetate, methanol and distilled water as a solvent. Antimicrobial activity of four extracts was performed by agar well diffusion method against different bacteria and fungi.

11p kethamoi6 29-06-2020 12 1   Download

The development of nanotechnology interests the researchers for synthesis of nanoparticles with various bio-applications. The green synthesis of copper nanoparticles using Momordica charantia fruit extract acts as both reducing and capping agent. The biosynthesized CuNps were characterized by using UV-Vis analysis, Dynamic Light Scattering (DLS), Fourier Transform Infrared analysis (FTIR), X-ray diffraction analysis (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) analysis.

11p kethamoi6 29-06-2020 10 2   Download

Honey has been used for various purposes including treatment of some diseases locally especially in wounds and upper respiratory tract infections (URTIs). There is paucity of information on the scientific basis for the use of honey in the treatment of upper respiratory tract infections caused by bacteria. This study was carried out to determine antibacterial activities of different brands of honey marketed in Ado-Ekiti and relate with those of some orthodox antibiotics used in the treatment of URTIs.

10p nguaconbaynhay6 24-06-2020 12 1   Download

Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates.

7p nguaconbaynhay6 23-06-2020 30 0   Download

Antibacterial susceptibility profiles of alum were determined against bacteria isolated from shellfish bivalve oysters. In-vitro bioassay using disc and agar well diffusion techniques with different concentrations; 1.5, 2.0, 2.5 and 3.0% of Alum were used to determine the susceptibility profiles of these isolates and compared with standard antibiotic, Ofloxacin (OFL) as control. Diameter of inhibition zones (DIZ) also were determined by measuring with a meter rule.

7p nguaconbaynhay6 23-06-2020 8 1   Download

The present study was conducted to asses phytochemical and antibacterial activity of various crude extracts viz, pet-ether, chloroform, methanol and aqueous extracts of Salvia aegyptiaca against bacteria and fungi such as Staphylococcus aureus (MTCC Code - 9886), Pseudomonas aeruginosa (MTCC Code - 6458), Aspergillus niger (MTCC Code - 872), Aspergillus flavus (MTCC Code - 8790) by adapting agar well diffusion method. The highest percentage of extraction yield was observed in aqueous extract followed by methanol, chloroform and petether extracts.

9p nguaconbaynhay6 23-06-2020 32 1   Download

The crude extract from the leaves of Acacia Arabica traditionally used in Indian system of medicines were screened against Escherichia coli NCIM 2931, Salmonella typhi MTCC 734, Salmonella typhimurium MTCC 98, Klebsiella pneumoniae MTCC432, Proteus vulgaris NCIM2857, Proteus mirabilis MTCC425, Pseudomonas aeruginosa NCIM5029, Staphylococcus aureus MTCC 96, Staphylococcus epidermis MTCC 435, Bacillus cereus NCIM2155, Bacillus subtilis NCIM 2063 and Bacillus megaterium NCIM 2087 by using agar well diffusion method.

7p angicungduoc5 12-06-2020 16 1   Download