Anaerobic

I. Khái niệm UASB viết tắt của Upflow Anaerobic Sludge Blanket (bể Lọc sinh học kỵ khí dòng chảy ngược qua lớp đệm bùn) Vi sinh vật học bao gồm 2 khái niệm: Vi sinh vật (Microoganisms) và Vi sinh học (Microbiology) Vi sinh học là khoa học nghiên cứu về các đặc điểm sinh học của các vi sinh vật. Đó là các sinh vật có kích thước hiển vi và siêu hiển vi

81p thiendi91 15-04-2011 445 160   Download

Early metabolic steps, including glycolysis and the activity of the pyruvate dehydrogenase complex, yield a two-carbon fragment called an acetyl group, which is linked to a large cofactor known as coenzyme A (or CoA). It is during the citric acid cycle that acetyl-CoA is oxidized to the waste product, carbon dioxide, along with the reduction of the cofactors NAD+ and ubiquinone

94p zingzing09 21-10-2012 58 9   Download

The aim of this project is to provide a summary of the current knowledge on energy recovery options from wastewater treatment plants sludge and/or biosolids. This covers technologies as well as data from case studies and research experimental results. The focus of the study is the ATP. The project methodology comprised two stages. The first stage involved a review of the current literature.

166p runthenight07 01-03-2023 8 3   Download

The aim of this study is to enhance biogas production from the organic fraction of municipal solid waste (OFMSW) and garden waste (GW) via pretreatment and codigestion. In order to achieve the aims of the study, two objectives have been developed and are outlined below: To investigate an optimum co-digestion mixture of OFMSW with untreated and pretreated GW; to investigate the effectiveness of different pretreatments on the potential for biogas production from GW and to identify the relationship between certain changes in GW characteristics and enhanced yield.

150p runthenight04 02-02-2023 8 2   Download

The quantity of rice straw and straw management survey only did in WinterSpring and Fall-Winter crops in An Giang, Dong Thap, Kien Giang province and Can Tho City. The research focused on the evaluation of simply biological pretreatment, material size and mixing rate of rice straw/hyacinth with pig manure in anaerobic batch and semi-continuous condition in vitro; trial producing biogas in polyethylene digester type (PE) in household scale in vivo

24p beloveinhouse10 28-11-2021 17 3   Download

This thesis focuses on simulating the internal circulation process (gas pulling water) to determine: The amount of water (QN) was pulled up by the amount of gas (QK); and mixing ability of generated gas and circulating water flow, from which to calculate the periodic structure in the IC system.

27p capheviahe26 25-01-2021 22 4   Download

Alkaline pulping liquors are the problematic for environment due to their toxicity and poor biodegradability. During last decade, anaerobic treatment technology has been successfully applied to various types of pulp and paper industry effluents. In the present study, the treatment of highly toxic pulping liquors in lab-scale has been done using sulfate reducing bacteria (SRB) isolated from sediment samples of pulp and paper mills in Vietnam. This treatment could reduce 70 - 75% of COD after three weeks. The COD removal could achieve 82 - 88% by subsequent aerobic treatment in 48 hours.

7p 12120609 01-06-2020 8 1   Download

This research aimed to study the effect of salt (NaCl) concentration on the treatment efficiency of seafood processing wastewater by the use of a laboratory-scale bioreactor, which is operated in anaerobic combining aerobic system with concentration salt different from 0- 5%.

10p tocectocec 25-05-2020 13 2   Download

The expression of mitochondrial and hydrogenosomal ADP/ATP carriers (AACs) from plants, rat and the anaerobic chytridiomycete fungus Neocallimastix spec. L2 in Escherichia coli allows a functional integration of the recombinant proteins into the bacterial cytoplasmic membrane. For AAC1 and AAC2 from rat, apparent Km values of about 40 lM for ADP, and 105 lM or 140 lM, respectively, for ATP have been determined, similar to the data reported for isolated rat mitochondria.

10p system191 01-06-2013 37 3   Download

Quercetin 2,3-dioxygenase (2,3QD) is a copper-containing dioxygenase that catalyses the oxidation of the flavonol quercetin to 2-protocatechuoylphloroglucinol carboxylic acid with concomitant production of carbon monoxide. In contrast to iron dioxygenases, very little is known about copper dioxygenases. We have characterized 2,3QD from the fungus Aspergillus japonicus by electron paramagnetic resonance spectroscopy (EPR). At pH 6.0, 2,3QD shows a mixture of two EPR species. The major form has parameters typical of type 2 Cu sites (g// ¼ 2.330, A// ¼ 13.

9p system191 01-06-2013 32 7   Download

Two novel chondroitinases, chondroitin ABC lyase (EC 4.2.2.4) and chondroitin AC lyase (EC 4.2.2.5), have been purified from Bacteroides stercoris HJ-15, which was isolated from human intestinal bacteria with glycosaminoglycan degrading enzymes. Chondroitin ABC lyase was purified to apparent homogeneity by a combination of QAE-cellulose, CM-Sephadex C-50, hydroxyapatite and Sephacryl S-300 column chromatography with a final specific activity of 45.7 lmolÆmin)1Æmg)1.

7p system191 01-06-2013 50 5   Download

The Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio alginolyticus was inactivated by reactive oxygen species. Highest Na+-NQR activity was observed in anaerobically prepared membranes that exhibited 1 : 1 coupling of NADH oxidation and Q reduction activities (1.6 UÆmg)1). Optical and EPR spectroscopy documented the presence of b-type cytochromes, a [2Fe)2S] cluster and an organic radical signal in anaerobically pre- pared membranes from V. alginolyticus.

6p research12 01-06-2013 48 4   Download

Chlamydomonas reinhardtii, a unicellular green alga, contains a hydrogenase enzyme, which is induced by anaerobic adaptation of the cells. Using the suppression subtractive hybridization (SSH) approach, the differential expression of genes under anaerobiosis was analyzed. A PCR fragment with similarity to the genes of bacterial Fe-hydrogenases was isolated and used to screen an anaerobic cDNA expression library of C. reinhardtii.

11p research12 01-06-2013 42 6   Download

In Klebsiella pneumoniae, the flavoprotein, NifL regulates NifA mediated transcriptional activation of the N2-fixation (nif) genes in response tomolecular O2and ammonium.We investigated the influence of membrane-bound oxidoreduc-tases on nif-regulation by biochemical analysis of purified NifLandbymonitoringNifA-mediated expressionofnifH¢-¢lacZ reporter fusions in different mutant backgrounds. NifL-bound FAD-cofactor was reduced by NADH only in the presence of a redox-mediator or inside-out vesicles derived from anaerobically grown K.pneumoniaecells,...

12p tumor12 20-04-2013 33 4   Download

The kinetic properties of glycine oxidase fromBacillus sub-tiliswere investigated using glycine, sarcosine, andD-proline as substrate. The turnover numbers at saturating substrate andoxygen concentrationswere 4.0 s )1 ,4.2s )1 ,and3.5 s )1 , respectively, with glycine, sarcosine, andD-proline as sub-strate. Glycine oxidase was converted to a two-electron reduced form upon anaerobic reduction with the individual substrates and its reductive half-reaction was demonstrated tobe reversible.

9p tumor12 20-04-2013 38 4   Download

We have isolated and characterized a second [Fe]-hydro-genasegene fromthegreenalga,Chlamydomonas reinhardtii. TheHydA2gene encodes a protein of 505 amino acids that is 74% similar and 68% identical to the known HydA1 hydrogenase fromC. reinhardtii.HydA2 contains all the conserved residues and motifs found in the catalytic core of the family of [Fe]-hydrogenases. We demonstrate that both theHydA1and theHydA2transcripts are expressed upon anaerobic induction, achieved either by neutral gas purging or by sulfur deprivation of the cultures. ...

9p fptmusic 16-04-2013 47 2   Download

It has long been accepted that bacterial quinol-fumarate reductase (QFR)generally uses a low-redox-potential naphthoquinone, menaquinone (MK), as the electron donor, whereas mitochondrial QFR from facultative and anaerobic eukaryotes uses a low-redox-potential benzoqui-none, rhodoquinone (RQ), as the substrate. In the present study, we purifiednovel complex II fromtheRQ-containing phototrophic purple bacterium,Rhodoferax fermentansthat exhibited high rhodoquinol-fumarate reductase activity in addition to succinate-ubiquinone reductase activity....

12p fptmusic 16-04-2013 39 3   Download

The membrane lipid composition of planctomycetes capable of the an-aerobic oxidation of ammonium (anammox), i.e. Candidatus‘Brocadia anammoxidans’ and Candidatus‘Kuenenia stuttgartiensis’, was shown to be composed mainly of so-called ladderane lipids. These lipids are com-prised of three to five linearly concatenated cyclobutane moieties withcis ring junctions, which occurred as fatty acids, fatty alcohols, alkyl glycerol monoethers, dialkyl glycerol diethers and mixed glycerol ether⁄esters. ...

14p fptmusic 11-04-2013 63 6   Download

We have cloned NADH oxidase homologues fromPyrococcus horikoshii and P. furiosus, and purified the recombinant form of the P. horikoshii enzyme to homogeneity from Escherichia coli. Both enzymes (previously referred to as NOX2) have been shown to act as a coenzyme A disulfide reductases (CoADR: CoA-S-S-CoA + NAD(P)H + H +fi2CoA-SH + NAD(P) + ). The P. horikoshiienzyme shows a kcat app of 7.2 s )1 with NADPH at 75
C.

0p awards 06-04-2013 38 4   Download

In recent studies on heme-copper oxidases a particular glutamate residue in subunit II has been suggested to constitute the entry point of the so-called K pathway. In contrast, mutations of this residue (E78 II ) in the Paracoccus denitrificanscytochromecoxidase do not affect its catalytic activity at all (E78 II Q) or reduce it to about 50% (E78 II A); in the latter case, the mutation causes no drastic decrease in hemea3 reduction kinetics under anaerobic con-ditions, when compared to typical K pathway mutants....

9p awards 05-04-2013 29 4   Download