Animal protein

Vibrio parahaemolyticus is one of the dangerous pathogens of aquatic animals, including fish. The outer membrane proteins play an essential role in bacterial virulence and are potential candidates for vaccine development. In this study, the virulence of four V. parahaemolyticus strains was studied, and the result was that strain. The V. parahaemolyticus N9 has the strongest virulence with an LD50 of 106.15 CFU/mL.

8p viinuzuka 28-02-2025 5 1   Download

This study explores the potential of chickpea protein as a nutritious alternative to animal protein. The study investigated the effects of water/powder ratio, ultrasound, and coagulation methods on chickpea protein recovery efficiency.

6p viyamanaka 06-02-2025 3 1   Download

he overall aim of this thesis was to improve nutritive value of cassava roots by fermentation yeast (Saccharomyces cerevisiae), Urea and di-ammonium phosphate additive and its utilization as protein source in the diets of Moo Lath pigs.

132p nguathienthan12 23-05-2021 18 3   Download

The study aimed at the utilization of locally available feed resources for increasing growth performance and reducing enteric methane emissions from goats in Lao PDR.

34p nguathienthan12 23-05-2021 30 3   Download

The main findings of the study were that (i) gas production and methane content of the gas were reduced when ensiled cassava root replaced the dried cassava root as a carbohydrate source, and when cassava leaf meal replaced water spinach meal as a protein source; (ii) Adding brewers’ grains at 5% dry matter (DM) to the diet of ensiled cassava root supplemented with either cassava foliage or water spinach as the main protein source increased DM feed intake...

193p nguathienthan12 23-05-2021 29 3   Download

The results of microbiological and toxicological tests have proved the safety of the bone-muscular cod waste (BMW). Waste (humerus with meat cuts) from cod cutting on fillets contains 18,95 % of a full-grade animal protein and insignificant amount of fat (0.15 %).

14p lucastanguyen 01-06-2020 21 2   Download

Quantifying the multiple processes which control and modulate the extent of oral bioavailability for drug candidates is critical to accurate projection of human pharmacokinetics (PK). Understanding how gut wall metabolism and hepatic elimination factor into first-pass clearance of drugs has improved enormously. Typically, the cytochrome P450s, uridine 5′- diphosphate-glucuronosyltransferases and sulfotransferases, are the main enzyme classes responsible for drug metabolism. Knowledge of the isoforms functionally expressed within organs of first-pass clearance, their anatomical topology (e.

16p caothientrangnguyen 09-05-2020 23 1   Download

Members of the proline-rich antibacterial peptide family, pyrrhocoricin, apidaecin and drosocin appear to kill responsive bacterial species by binding to themultihelical lid region of the bacterial DnaK protein.Pyrrhocoricin, the most potent among these peptides, is nontoxic to healthy mice, andcanprotect these animals frombacterial challenge.

12p research12 23-04-2013 35 3   Download

Intrinsic factor (IF) is the gastric protein that promotes the intestinal uptake of vitamin B12 . Gastric IF from animal sources is used in diagnostic tests and in vitamin pills. However, administration of animal IF to humans becomes disadvantageous because of possible pathogenic transmis-sion and contamination by other B12binders. We tested the use of recombinant plants for large-scale production of pathogen-free human recombinant IF. Human IF was successfully expressed in the recombinant plantArabidopsis thaliana....

6p tumor12 20-04-2013 42 3   Download

A number of proteins related to the homotetrameric trans-port protein transthyretin (TTR) forms a highly conserved protein family, whichwe present in an integrated analysis of data from different sources combined with an initial bio-chemical characterization.Homologues of the transthyretin-relatedprotein (TRP) canbe found inawide rangeof species including bacteria, plants and animals, whereas transthyre-tinshave so faronlybeen identified invertebrates.

15p tumor12 20-04-2013 49 3   Download

Transmissible spongiform encephalopathies (TSE) are fatal neuro-degenerative diseases of humans and animals. The underlying infectious agent, the prion, accumulates not only in the central ner-vous system (CNS) but also in secondary lymphoid organs. I will revisit the role of the immune system in peripheral prion pathogen-esis, while focusing on the mechanisms by which extraneural and extralymphatic prion infectivity develops.

64p fptmusic 11-04-2013 37 4   Download

Protein aggregation is central to most neurodegenerative diseases, as shown by familial case studies and by animal models. A modified ‘amyloid cas-cade’ hypothesis for Alzheimer’s disease states that prefibrillar oligomers, also called amyloid-b-derived diffusible ligands or globular oligomers, are the responsible toxic agent. It has been proposed that these oligomeric spe-cies, as shown for amyloid-b, b2 -microglobulin or prion fragments, exert toxicity by forming pores in membranes, initiating a cascade of detrimental events for the cell. ...

10p awards 06-04-2013 43 3   Download

In many animal species includingXenopus, ovulated eggs possess an intrin-sic apoptotic execution system. This program is inhibited for a limited time by some maternal apoptosis inhibitors, although their molecular properties remain uncharacterized. Baculovirus IAP repeat (BIR) family proteins con-tain evolutionarily conserved BIR domains and play important roles in apoptosis suppression, and are therefore good candidates as maternal apoptosis inhibitors.

14p awards 06-04-2013 39 4   Download

Planarians are one of the simplest animal groups with a central nervous system. Their primitive central nervous system produces large quantities of a variety of neuropeptides, of which many are amidated at their C terminus. In vertebrates, peptide amidation is catalyzed by two enzymes [peptidylglycine a-hydroxylating monooxygenase (PHM) and peptidyl-a-hydroxylglycinea-amidating lyase] acting sequentially. In mammals, both enzymatic activities are contained within a single protein that is encoded by a single gene....

14p awards 05-04-2013 43 4   Download

The conduction of protons through human Nox2 has pre-viously been shown to be dependent upon His115. Align-ment of sequences for both animal and plant Nox proteins indicated that histidines 115 and 119 are both highly con-served, while His111 was conserved among animal homo-logues of Nox1–4. To investigate the possible role that these histidine residues might play in the conduction of protons through Nox2, we have introduced both paired and single mutations into these histidine residues.

8p awards 05-04-2013 45 4   Download

During a search for cDNAs encoding plant sterol acyl-transferases, we isolated four full-length cDNAs fromAra-bidopsis thaliana that encode proteins with substantial identity with animal lecithin : cholesterol acyltransferases (LCATs). The expressionof one of these cDNAs,AtLCAT3 (At3g03310), invarious yeast strains resulted in the doubling of the triacylglycerol content.

13p awards 05-04-2013 42 4   Download

Experimental autoimmune encephalomyelitis can be induced in susceptible animals by immunodominant deter-minants of myelin basic protein (MBP). To characterize the molecular features of antigenic sites important for designing experimental autoimmune encephalomyelitis suppressing molecules, we report structural studies, based on NMR experimental data in conjunction with molecular dynamic simulations, of the potent linear dodecapeptide epitope of guinea pig MBP, Gln74-Lys75-Ser76-Gln77-Arg78-Ser79-Gln80-Asp81-Glu82-Asn83-Pro84-Val85 [MBP(74–85)], and its antagonist analogue Ala81MBP(74–85)....

15p awards 05-04-2013 56 3   Download

Collectins are a family of collagenous calcium-dependent defense lectins in animals. Their polypeptide chains consist of four regions: a cysteine-rich N-terminal domain,a colla-gen-like region,ana-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain. These polypeptide chains form trimers that may assemble into larger oligomers. The best studied family members are the mannan-binding lectin,which is secreted into the blood by the liver,and the surfactant proteins A andD,which are secreted into the pulmonary alveolar and airway lining fluid....

21p dell39 03-04-2013 50 6   Download

Spermine oxidase (SMO) is a flavoenzyme involved in polyamine homeostasis in animal cells. Themouse spermine oxidasegene (mSMO) codes for splicevariants, including the previously reportedmajor active isoform, herein named alfa (a). In the present work, eight additional gene splicing variants were characterized. The heterologous expression and biochemical characterization ofthree recombinant iso-forms (namely mSMOl,-cand -d) revealed that only the recombinant protein mSMOldisplays biochemical charac-...

11p dell39 03-04-2013 45 5   Download

On the basis of a partial N-terminal sequence, Jolle`s and Jolle`s [Jolle`s, J., & Jolle`s, P. (1975)Eur. J. Biochem.54, 19–23] previously proposed that the lysozyme from the starfishAsterias rubensrepresents a new form of lysozyme, called typei(invertebrate) lysozyme. Indeed, it differed from both the typesc(chicken) and g(goose) known in other animals, as well as from plant and phage lysozymes. Recently, several proteins belonging to the same family have been isolated from protostomes. Here we report the com-plete mature protein sequence and cDNA sequence of the lysozyme from Asterias. ...

6p dell39 03-04-2013 45 3   Download