Applications in medicine

The study clarified the main chemical composition of the roots of F. multiflora. Studying and developing a process of qualitative and quantitative analysis of several active ingredients at the same time. Application of analytical procedure has established and evaluated the content of active ingredients in some F. multiflora medicinal samples. The research results are obtained as a scientific basis for further studies.

23p extraenglish 24-05-2021 26 2   Download

Describe clinico-pathological characteristics and procedure of endoscopic thyroidectomy for treatment of early differentiated thyroid cancer in National Hospital of Endocrinology. Evaluate results of endoscopic thyroidectomy for treatment of early differentiated thyroid cancer in National Hospital of Endocrinology.

27p trinhthamhodang7 31-08-2020 28 3   Download

The main aim of this thesis is to overcome the second limitation of the automatic plant identification (low recognition accuracy) by proposing novel and robust methods for plant recognition. For this, we first focus on improving the recognition accuracy of plant identification based on images of one sole organ. Among different organs of the plant, we select leaf as this organ is the most widely in the literature.

27p gaocaolon6 30-07-2020 29 3   Download

Describe clinico-pathological characteristics and procedure of endoscopic thyroidectomy for treatment of early differentiated thyroid cancer in National Hospital of Endocrinology. Describe clinico-pathological characteristics and procedure of endoscopic thyroidectomy for treatment of early differentiated thyroid cancer in National Hospital of Endocrinology.

27p angicungduoc6 21-07-2020 30 4   Download

Research the results of the application of follow-up and diagnosis of nodules in the lungs of Mayo Clinic hospital-United States after 3-6 months.

31p angicungduoc6 21-07-2020 33 3   Download

Precision medicine promises to improve both the efficacy and safety of therapeutic products by better informing why some patients respond well to a drug, and some experience adverse reactions, while others do not. Pharmacogenomics is a key component of precision medicine and can be utilized to select optimal doses for patients, more precisely identify individuals who will respond to a treatment and avoid serious drug-related toxicities.

5p caothientrangnguyen 09-05-2020 11 2   Download

Development of optimized pediatric formulations for oral administration can be challenging, time consuming, and financially intensive process. Since its inception, the biopharmaceutical classification system (BCS) has facilitated the development of oral drug formulations destined for adults. At least theoretically, the BCS principles are applied also to pediatrics. A comprehensive age-appropriate BCS has not been fully developed.

9p caothientrangnguyen 09-05-2020 36 3   Download

So we conducted this study aiming at: Describe the current situation of clinical teaching-learning in fulltime general practitioner students at Hai Phong University of Medicine and Pharmacy in 2014 and some influencing factors. Evaluating the results of the application of a number of pilot clinical t

27p cotithanh321 06-08-2019 33 2   Download

The goad of the thesis is to build the manufacture process of nano-sized magnetic fluids based on iron oxide (uniform particle size and high magnetic saturation) with stable technology; Characteristic research of magnetic properties of magnetic nanoparticles; assessment of toxicity and test of effects on cells, aiming to make contrast medicine in imaging diagnosis by magnetic resonance imaging (MRI), application on accurately identifying cancer.

28p xacxuoc4321 08-07-2019 45 5   Download

The design of b-glycosidases with planed substrate specificity for biotechnological application has received little attention. This is mostly a consequence of the lack of data on the molecular basis of the b-glycosidase specificity, namely data on the energy of the noncovalent interactions in the enzymetransition state complex. In an attempt to fill this gap, sitedirected mutagenesis and enzyme steady-state kinetic experiments with different substrates were conducted, using as model a digestive b-glycosidase (glycoside hydrolase family 1) from Spodoptera frugiperda (Lepidoptera) (Sfbgly50).

10p system191 01-06-2013 41 4   Download

An immuno-homologue of maize Ca2+/calmodulin (CaM)dependent protein kinase with a molecular mass of 72 kDa was identified in pea. The pea kinase (PsCCaMK) was upregulated in roots in response to low temperature and increased salinity. Exogenous Ca2+ application increased the kinase level and the response was faster than that obtained following stress application. Low temperaturemediated, but not salinity-mediated stress kinase increase was inhibited by the application of EGTA and W7, a CaM inhibitor....

12p system191 01-06-2013 39 5   Download

Cytochrome c is widely distributed in bacterial species, from mesophiles to thermophiles, and is one of the best-characterized redox proteins in terms of biogenesis, folding, structure, function, and evolution. Experimental molecular biology techniques (gene cloning and expression) have become applicable to cytochrome c, enabling its engineering and manipulation. Heterologous expression systems for cytochromes c in bacteria, for use in mutagenesis studies, have been established by extensive investigation of the biological process by which the functional structure is formed. ...

7p research12 01-06-2013 32 3   Download

Structural analysis of the lipopolysaccharide (LPS) of nontypeableHaemophilus in¯uenzaestrain 1003 has been achieved by the application of high-®eld NMR techniques, ESI-MS, capillary electrophoresis coupled to ESI-MS, composition and linkage analyses on O-deacylated LPS and core oligosaccharide material. It was found that the LPS contains the common structural element of H.

11p research12 01-06-2013 47 7   Download

The nitrilase AtNIT1 fromArabidopsis thalianawas over-expressed inEscherichia coliwith an N-terminal His6tag and puri®ed by zinc chelate anity chromatography in a single step almost to homogeneity in a 68% yield with a speci®c activity of 34.1 Uámg )1 . The native enzyme (450 kDa) consists of 11±13 subunits (38 kDa). The temperature optimum was determined to be 35°C, and a pH optimum of 9 was found.

8p research12 23-04-2013 27 2   Download

Pycnoporus cinnabarinuslaccaselac1genewasoverexpressed in Aspergillus niger, a well-known fungal host producing a large amount of homologous or heterologous enzymes for industrial applications. The corresponding cDNA was placed under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter as a strong and constitutive pro-moter. The laccase signal peptide or the glucoamylase preprosequence ofA.

8p research12 23-04-2013 51 5   Download

As one of the most important families of non-natural poly-mers with the propensity to form well-defined secondary structures, theb-peptides are attracting increasing attention. The compounds incorporatingb-amino acid residues have found various applications in medicinal chemistry and biochemistry.The conformational pool ofb-peptides com-prises several periodic folded conformations, which can be classified as helices, and nonpolar and polar strands.The latter two are prone to form pleated sheets.

10p tumor12 20-04-2013 30 4   Download

Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleo-tide. These analogs have strong binding affinities for most nucleotide-requiring systems. Their bindings are easily detected by absorption and fluorescence changes in the visi-ble region.Recent years have seendramatic developments in the application of the TNP nucleotide analogs as spectro-scopic probes for the study on the nucleotide-interacting properties of various enzymes and proteins including their mutants.

7p tumor12 20-04-2013 38 3   Download

The Tet repressor protein (TetR) regulates transcription of a family of tetracycline (tc) resistance determinants in Gram-negative bacteria. The resistance protein TetA, a membrane-spanning H + -[tcÆM]+ antiporter, must be sen-sitively regulated because its expression is harmful in the absence of tc, yet it has to be expressed before the drugs’ concentration reaches cytoplasmic levels inhibitory for protein synthesis.

13p tumor12 20-04-2013 40 4   Download

Here, we show that recombinant bovine PDE5A1 is pro-teolysedby recombinant caspase-3 inin vitroand transfected Cos-7 cells. In addition, the treatment of PDE5A1-trans-fectedCos-7andPC12cellswithstaurosporine, anapoptotic agent that activates endogenous caspase-3, also induced proteolysis and inactivation of PDE5A1. These findings suggest that there is specificity in the interaction between caspase-3 and PDE5A1 that requires application of an apoptotic stimulus.

9p tumor12 20-04-2013 57 5   Download

Molecular mechanics calculations on conformers of Ac-HGly-NHMe, Ac-b 2 -HAla-NHMe and Ac-b 3 -HAla-NHMe indicate that low-energy conformations of the b-amino acids backbone, corresponding to gauche rotamers around the Ca–Cbbond, may overlap canonical backbone conformers observed for a-amino acids. Therefore, Sub-stance P (SP) was used as a model peptide to analyse the structural and biological consequences of the substitution of Phe7andPhe8by(R)-b 2 -HPheandofGly9byHGly (R)-b 2 -HAla or (S)-b 3 -HAla....

11p tumor12 20-04-2013 33 3   Download