Crude extract

This study aimed to isolate and identify marine fungi from Bai Tu Long Bay and assess their antimicrobial potential. We successfully isolated twenty strains of marine-derived fungi. The crude extracts from these fungi were tested against pathogenic microorganisms. All twenty strains exhibit some degree of growth inhibition against the tested microorganisms.

9p dianmotminh02 03-05-2024 5 2   Download

Zoanthid is a potential natural resource with many biological activities, such as antioxidant, antiviral, antiinflammatory, and cytotoxic activity. Therefore, this study aimed to evaluate the antioxidant activity of 70% ethanol crude extract and dichloromethane fraction from Zoanthus vietnamensis. The total polyphenol content of ethanol and dichloromethane extracts were 19.36 ± 1.37 and 24.95 ± 0.63 mg GAE/g extract, respectively.

9p dianmotminh02 03-05-2024 7 3   Download

Develop the process of producing crude extracts, fractions and pure chemical compounds isolated from E. fortunei; tudy of inhibitory effect of the crude ethanol extracts from E. fortunei on the growth of M.aeruginosa and evaluating their ecological safety to non-target aquatic organisms.

28p larachdumlanat129 20-01-2021 28 3   Download

The maceration was applied to the seeds of the plant to afford the crude extract which was then fractionated by liquid-liquid extraction to obtain the ethyl acetate extract. The extract was carried out by using normal phase silica gel column chromatography and thin-layer chromatography. Analysis of spectroscopic data including HR-ESI-MS, 1D and 2D-NMR and a comparison of the NMR data with that in the literature led to the structural elucidation of isolated compounds.

4p larachdumlanat127 02-01-2021 12 2   Download

In this study, methanolic extracts from the stems, leaves and flowers of Ipomoea pes-caprae that were collected in Xuan Thuy National Park were evaluated for their antibacterial and antioxidative activities, and their ability to inhibit plant growth. The DPPH free radical scavenging activity of crude methanol extracts was evaluated.

7p tamynhan8 04-11-2020 20 2   Download

Recently, we collected fruit bodies of Ganoderma neo-japonicum in Lamdong Province and found that the EtOAc crude extract obtained, having an IC50 value of 19.14 µg/mL, shows strong cytotoxicity against the KB cell line. This paper describes the isolation and cytotoxicity of four compounds from this mushroom.

5p tamynhan8 04-11-2020 19 1   Download

The objective of the present investigation was to isolate and identify the antimicrobial activity showing actinomycetes associated with sponges. Sponges such as Acanthella klethra, Theonella cylendrica, Helicona sp., Auletta sp., and Callyspongia sp. were collected at Sangumal, in Rameswaram island, Ramanathapuram District, Tamil Nadu, India. Isolates of 22 actinomycetes were isolated from sponges. Isolates No. 16 showed high antibacterial and antifungal activity and the isolate was identified as Streptomyces sp.

8p cothumenhmong5 17-05-2020 13 1   Download

Natural precious products such as aroma compounds, essential oils, and bio-activated materials are usually extracted from about 30,000 botanical species. These extracts are often high competitive market due to their small content (less than 1 %) in plants and high purification cost. Thus, development of a modeling for the optimization of the crude oil extraction is highly paid attention. In this work, a modeling of Vietnam lemongrass oil extraction using steam distillation is developed and the optimization of the process parameters is performed using response surface methodology (RSM).

6p abcxyz123_02 03-03-2020 31 2   Download

Department of Biochemistry, University of Rajshahi, Rajshahi-6205. Bangladesh Three lectins were extracted and purified from mulberry seeds by gel filtration of 100% ammonium sulfate saturated crude protein extract followed by ion-exchange chromatography on DEAE and CM-cellulose. The lectins were found to be homogeneous as judged by polyacrylamide disc gel electrophoresis. The molecular masses of the lectins as determined by gel filtration were 175 000 for MSL-1, 120 000 for MSL-2 and 89 500 for MSL-3....

6p system191 01-06-2013 36 5   Download

From the antennae of the moth Mamestra brassicae, we have identified a lepidopteran G protein a subunit belonging to the Gq family, through immunological detection in crude antennal extract and antennal primary cell cultures, followed by molecular cloning. The complete cDNA sequence (1540 bp) contains an open reading frame encoding a protein of 353 amino acids.

10p research12 01-06-2013 26 4   Download

An extracellular lipase, LipA, extracted fromAcinetobacter sp. RAG-1 grown on hexadecane was purified and proper-ties of the enzyme investigated. The enzyme is released into the growth medium during the transition to stationary phase. The lipase was harvested from cells grown to sta-tionary phase, and purified with 22% yield and 10-fold purification. The protein demonstrates little affinity for anionexchange resins,withcontaminatingproteins removed by passing crude supernatants over a Mono Q column....

9p tumor12 22-04-2013 47 2   Download

A novel nucleosidase enzymatic activity was discovered in the marine spongeAxinella polypoides. This enzyme, desig-nated as ATP N-glycosidase, converts adenosine-5¢-tri-phosphate into adenine and ribose-5-triphosphate. The crude extract ofA.polypoideswas capable of hydrolysing 25lmol ATPÆmin )1 per g wet weight of sponge. The cata-lytic activity of a sponge crude extract permg total protein is comparable with specific activities of purified plant adeno-sine and bacterial AMP nucleosidases.

11p tumor12 20-04-2013 55 3   Download

The isopenicillin N acyltransferases (IATs) ofAspergillus nidulansandPenicilliumchrysogenumdiffered in their ability to maintain the 40-kDa proacyltransferaseabheterodimer in an undissociated form. The native A. nidulansIAT exhibited a molecular mass of 40 kDa by gel filtration. The P. chrysogenumIATshowedamolecularmassof 29 kDaby gel filtration (corresponding to the b subunit of the enzyme) but the undissociated 40-kDa heterodimer was never observed even in crude extracts.

11p fptmusic 16-04-2013 41 5   Download

Two distinguishable activity bands for dye-linkedl-proline dehydrogenase (PDH1 and PDH2) were detected when crude extract of the hyperthermo-philic archaeonPyrococcus horikoshiiOT-3 was run on a polyacrylamide gel. After purification, PDH1 was found to be composed of two different subunits with molecular masses of 56 and 43 kDa, whereas PDH2 was composed of four different subunits with molecular masses of 52, 46, 20 and 8 kDa.

11p fptmusic 11-04-2013 30 1   Download

2-Amino-5-carboxymuconic 6-semialdehyde is an unstable intermediate in the meta-cleavage pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d.In vitro, this compound is nonenzymatically converted to 2,5-pyrid-inedicarboxylic acid. Crude extracts of strain 10d grown on 4-amino-3-hydroxybenzoic acid converted 2-amino-5-car-boxymuconic 6-semialdehyde formed from 4-amino-3-hydroxybenzoic acid by the first enzyme in the pathway, 4-amino-3-hydroxybenzoate 2,3-dioxygenase, to a yellow compound (emax¼375 nm).

0p awards 05-04-2013 45 2   Download

Crude rat liver extract showed AMP–AMP phosphotransferase activity which, on purification, was ascribed to a novel interaction between adeny-late kinase, also known as myokinase (EC 2.7.4.3), and adenosine kinase (EC 2.7.1.20). The activity was duplicated using the same enzymes purified from recombinant sources.

15p vinaphone15 27-02-2013 28 2   Download

After screening 25 marine invertebrates, a novel metallocarboxypeptidase (SmCP) has been identified by activity and MS analytical approaches, and isolated from the marine annelid Sabellastarte magnifica. The enzyme, which is a minor component of the molecularly complex animal body, as shown by 2D gel electrophoresis, has been purified from crude extracts to homogeneity by affinity chromatography on potato carboxypeptidase inhib-itor and by ion exchange chromatography.

16p vinaphone15 25-02-2013 35 1   Download