Endonuclease

1.1. Restriction endonuclease enzyme (RE) 1962: hạn chế sự sinh sản của phage trong tế bào vi khuẩn Hệ thống hạn chế - biến đổi (restriction – modification system) giúp bảo vệ tế bào khỏi sự xâm nhập của DNA lạ. Trình tự nhận biết: trình tự 4-6 cặp nu đối xứng đảo ngược (palindrom) Cắt tạo thành đầu so le (sticky end) hay đầu bằng (cohesive end) .

45p htc_12 13-05-2013 48 9   Download

Bài giảng Sinh học phân tử: Chương 7 trình bày về kỹ thuật tạo dòng. Chương này trình bày những nội dung chính sau: Các công cụ sử dụng trong kỹ thuật tạo dòng, Restriction Enzyme (RE), vai trò sinh học của RE, restriction endonuclease, tạo dòng phân tử (molecular cloning), DNA tái tổ hợp, công cụ tạo dòng,... Mời các bạn tham khảo.

34p hihihaha2 03-12-2016 101 12   Download

The two group I introns Nae.L1926 and Nmo.L2563, found at two different sites in nuclear LSU rRNA genes of Naegleria amoebo-flagellates, have been characterized in vitro. Their structural organization is related to that of the mobile Physarum intron Ppo.L1925 (PpLSU3) with ORFs extending the L1-loop of a typical group IC1 ribozyme. Nae.L1926, Nmo.L2563 and Ppo.L1925 RNAs all selfsplice in vitro, generating ligated exons and full-length intron circles as well as internal processed excised intron RNAs....

9p system191 01-06-2013 31 5   Download

During starvation induced encystment, cells of the myxo-myceteDidymiumiridisaccumulate a7.5-kbRNAthat is the result of alternative processing of pre-rRNA. The 5¢ end corresponds to an internal processing site cleaved by the group I-like ribozyme DiGIR1, located within the twin-ribozyme intron Dir.S956-1. The RNAretains the majority ofDir.S956-1 including thehoming endonuclease geneanda small spliceosomal intron, the internal transcribed spacers ITS1 and ITS2, and the large subunit rRNAlacking its two group I introns. ...

9p tumor12 22-04-2013 30 2   Download

The RuvABC resolvasome ofEscherichia colicatalyses the resolution of Holliday junctions that arise during genetic recombination and DNA repair. This process involves two key steps: branch migration, catalysed by the RuvB protein that is targeted to the Holliday junction by the structure specific RuvAprotein, and resolution, which is catalysed by the RuvC endonuclease.

10p tumor12 22-04-2013 35 2   Download

Mammalian mitochondria contain strong nuclease activity. Endonuclease G (endoG), which predominantly resides in mitochondria, accounts for a large part of this nuclease activity. It has been proposed to act as an RNase H-like nuclease on RNAÆDNA hybrids (R-loops) in the D-loop region where the origins of mitochondrial replication are mapped, providing RNA primers for mtDNA replication.

6p tumor12 22-04-2013 38 2   Download

The AnCOB group I intron fromAspergillus nidulans encodes a homing DNA endonuclease called I-AniI which also functions as a maturase, assisting in AnCOB intron RNA splicing. In this investigation we biochemically char-acterized the endonuclease activity of I-AniIin vitro and utilizedcompetitionassays toprobe the relationshipbetween theRNA- andDNA-binding sites.

12p tumor12 20-04-2013 35 3   Download

Group I introns are thought to be self-propagating mobile elements, and are distributed over a wide range of organisms through horizontal trans-mission. Intron invasion is initiated through cleavage of a target DNA by a homing endonuclease encoded in an open reading frame (ORF) found within the intron. The intron is likely of no benefit to the host cell and is not maintained over time, leading to the accumulation of mutations after intron invasion.

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Endonuclease I is a periplasmic or extracellular enzyme present in many different Proteobacteria. TheendAgene encoding endonuclease I from the psychrophilic and mildly halophilic bacteriumVibrio salmonicidaand from the mesophilic brackish water bacterium Vibrio choleraehave been cloned, over-expressed inEscherichia coli, and purified.

12p inspiron33 23-03-2013 59 5   Download

Drosophila melanogasterXPG-like endonuclease (DmGEN) is a new cate-gory of nuclease belonging to the RAD2⁄XPG family. The DmGEN pro-tein has two nuclease domains (N and I domains) similar to XPG⁄class I nucleases; however, unlike class I nucleases, in DmGEN these two nuclease domains are positioned close to each other as in FEN-1⁄class II and EXO-1⁄ class III nucleases.

14p galaxyss3 21-03-2013 45 3   Download

Adaptation to extreme environments affects the stability and catalytic effi-ciency of enzymes, often endowing them with great industrial potential. We compared the environmental adaptation of the secreted endonuclease I from the cold-adapted marine fish pathogenVibrio salmonicida(VsEndA) and the human pathogen Vibrio cholerae (VcEndA).

13p media19 06-03-2013 43 3   Download

The genome of the human pathogenEntamoeba histolytica, a primitive pro-tist, contains non-long terminal repeat retrotransposable elements called EhLINEs. These encode reverse transcriptase and endonuclease required for retrotransposition. The endonuclease shows sequence similarity with bacterial restriction endonucleases.

13p vinaphone15 25-02-2013 36 2   Download

Apurinic⁄apyrimidinic endonuclease (APE), an essential DNA repair enzyme, initiates the base excision repair pathway by creating a nick 5¢ to an abasic site in double-stranded DNA. Although the Chinese hamster ovary cells remain an important model for DNA repair studies, the Chinese hamster APE (chAPE1) has not been studied in vitro in respect to its kinetic characteristics.

9p viettel02 19-02-2013 37 2   Download

Group II introns are both catalytic RNAs and mobile retroelements that move through a process catalyzed by a RNP complex consisting of an intron-encoded protein and the spliced intron lariat RNA. Group II intron-encoded proteins are multifunctional and contain an N-terminal reverse transcriptase domain, followed by a putative RNA-binding domain (domain X) associated with RNA splicing or maturase activity and a C-terminal DNA binding⁄DNA endonuclease region.

11p mobifone23 18-01-2013 37 4   Download

Tham khảo luận văn - đề án 'báo cáo khoa học: solution parameters modulating dna binding specificity of the restriction endonuclease ecorv', luận văn - báo cáo, báo cáo khoa học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

15p cosis54 05-01-2013 57 4   Download

The DNA binding stringency of restriction endonucleases is crucial for their proper function. The X-ray structures of the specific and non-cognate complexes of the restriction nucleaseEcoRV are considerably different sug-gesting significant differences in the hydration and binding free energies.

11p cosis54 05-01-2013 36 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: inherent potential for biosynthetic versus live recombinant microbicides

12p toshiba17 28-10-2011 61 3   Download

Cấu trúc virus HIV HIV có đặc điểm chung của họ retroviridae. Hạt virus hoàn chỉnh (virion) có cấu trúc gồm 3lớp. 1. Lớp vỏ ngoài (vỏ peplon): lớp này là 1 màng lipid kép có kháng nguyên chéo với màng vỏ capsid, bao gồm: + Hai phân tử ARN đơn, đó là bộ gen di truyền HIV(genom). Genom của HIV chứa 3 gen cấu trúc: Gag (group specific antigen) là cac gen mã hoá cho các kháng nguyên đặc hiệu của capsid cuûa virus; Pol (polymerase) mã hoá cho các Enzym: reverve transcriptase (RT:Enzym sao mã ngược), protease và endonuclease (còn...

2p kalynn 25-07-2011 453 82   Download

Enzyme giới hạn Enzyme giới hạn (restriction enzyme, RE) là một enzyme endonuclease có vị trí nhận biết điểm cắt DNA đặc hiệu. Những enzyme này phân huỷ liên kết phosphodieste của bộ khungDNA mạch đôi mà không gây tổn hại đến bases.

6p heoxinhkute7 30-12-2010 485 88   Download

Enzyme giới hạn (restriction enzyme, RE) là một enzyme endonuclease có vị trí nhận biết điểm cắt DNA đặc hiệu. Những enzyme này phân huỷ liên kết phosphodieste của bộ khung DNA mạch đôi mà không gây tổn hại đến bases.

8p heoxinhkute 27-07-2010 346 82   Download