Genetically engineered

This study introduces a practical and efficient methodology to improve the estimation of the ultimate axial bearing capacity of Pre-bored Grouted Planted Nodular (PGPN) piles, addressing a critical need in geotechnical engineering.

25p viyamanaka 06-02-2025 1 1   Download

Thuật ngữ ‘chuyển gien’ (Transgenic) dùng để chỉ việc đưa gien vào 1 đối tượng SV nào đó bằng KT ADN tai tổ hợp. Gien được chuyển từ các loài khác hay trong cùng 1 loài. Từ đồng nghĩa (Synonym): Genetically modified organism (GMO) Genetically engineered organism (GEO)

26p trada85 22-01-2013 71 9   Download

An applied field of biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring bioproducts. (http://en.wikipedia.org/wiki/Biotechnology) It is the products and exploitation from other fundamental sciences.Traditional Biotechnology Traditional biotechnology refers to a number of ancient ways of using living organisms to make new products or modify existing ones. In its broadest definition, traditional biotechnology can be traced back to human's transition from hunter-gatherer to farmer.

29p zingzing09 24-04-2013 64 9   Download

The research has designed a novel active truck seat suspension system for a further study of active vibration control. A 5-degree-of-freedom driver and seating suspension system model for active vibration control has been developed. A novel fast system parameter identification method from vibration measurement data has been proposed for the seat-occupant system based on the multi-objective Genetic Algorithm optimization (GA).

206p runthenight04 02-02-2023 13 5   Download

The objective of the thesis is to know about two species of SLP P.heterotremus and P. westermani, providing a scientific basis for the diagnosis and prevention of SLP disease, contributing to protecting public health.

27p xacxuoc4321 11-07-2019 57 4   Download

Division of Medicinal and Natural Products Chemistry, Department of Chemistry and Department of Chemical Engineering, University of Iowa, USA; 2Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark Synthetic peptides based on amino-acid residues 27–38 of human serum amyloid P component represent a novel type of heparin binders as they do not contain clusters of basic amino acids or other known features associated with protein or peptide heparin binding.

8p system191 01-06-2013 56 4   Download

The envelope protein gp64 of the baculovirusAutographa californicanuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein bothmediates pH-dependentmembrane fusionandbinds tohost cell receptors. Surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and powerful tool in molecular biology.

10p research12 23-04-2013 28 2   Download

Human butyrylcholinesterase (BChE; EC 3.1.1.8) is of particular interest because it hydrolyzes or scavenges a wide range of toxic compounds including cocaine, organophos-phorus pesticides andnerveagents.The relative contribution of each N-linked glycan for the solubility, the stability and the secretionof the enzymewas investigated. Arecombinant monomeric BChE lacking four out of nine N-glycosylation sites and the C-terminal oligomerization domain was stably expressed as a monomer in CHO cells.

8p research12 23-04-2013 31 4   Download

The (ba)8-barrel is the most common enzyme fold and it is capable of cata-lyzing an enormous diversity of reactions. It follows that this scaffold should be an ideal starting point for engineering novel enzymes by directed evolution. However, experiments to date have utilized in vivo screens or selections and the compatibility of (ba)8-barrels with in vitro selection methods remains largely untested.

14p fptmusic 11-04-2013 42 3   Download

The structure of the complex between the minor groove binder netropsin and d(GGCCAATTGG) was determined via single-crystal X-ray tech-niques. The structure was refined to completion usingrefmac5.1.24, result-ing in a residual R-factor of 20.0% (including 68 water molecules). Using crystal engineering and cryocooling techniques, the resolution could be enhanced to 1.75 A ˚ , resulting in an unambiguous determination of the drug conformation and orientation.

11p fptmusic 11-04-2013 35 2   Download

The efficient engineering of enzymes with novel activities remains an ongoing challenge. Towards this end, genetic selection techniques provide a method for finding rare solutions to catalytic problems that requires only a limited foreknowledge of structure–function relationships. We have used genetic selections to extensively probe the structure and mechanism of chorismate mutases. The insights gained from these investigations will aid future enzyme design efforts.

8p dell39 03-04-2013 51 4   Download

We show a simple and reliable method of tRNA aminoacylation with natural, as well as non-natural, amino acids at high pressure. Such specific and noncognate tRNAs can be used as valuable substrates for protein engineering. Aminoacylation yield at high pressure depends on the chem-ical nature of the amino acid used and it is up to 10%.

10p inspiron33 25-03-2013 46 3   Download

An approach based on anin silico analysis predicted that CYP77A4, a cytochrome P450 that so far has no identified function, might be a fatty acid-metabolizing enzyme. CYP77A4was heterologously expressed in a Saccharomyces cerevisiaestrain (WAT11) engineered for cytochrome P450 expression. Lauric acid (C12:0 ) was converted into a mixture of hydroxy-lauric acids when incubated with microsomes from yeast expressing CYP77A4.

17p vinaphone15 27-02-2013 46 2   Download

KcsA, a potassium channel fromStreptomyces lividans, was the first ion channel to have its transmembrane domain structure determined by crystal-lography. Previously we have shown that its C-terminal cytoplasmic domain is crucial for the thermostability and the expression of the channel. Expression was almost abolished in its absence, but could be rescued by the presence of an artificial left-handed coiled coil tetramerization domain GCN4.

11p viettel02 20-02-2013 48 3   Download

Our previous studies revealed that the double variant of cytochrome P450 (CYP)105A1, R73V⁄R84A, has a high ability to convert vitamin D3 to its biologically active form, 1a,25-dihydroxyvitamin D3 [1a,25(OH)2D3 ], suggesting the possibility for R73V⁄R84A to produce 1a,25(OH)2D3 . BecauseActinomycetes, including Streptomyces, exhibit properties that have potential advantages in the synthesis of secondary metabolites of industrial and medical importance

11p viettel02 19-02-2013 40 2   Download

The mammalian natriuretic peptide system, consisting of at least three ligands and three receptors, plays critical roles in health and disease. Exam-ination of genetically engineered animal models has suggested the signifi-cance of the natriuretic peptide system in cardiovascular, renal and skeletal homeostasis.

12p cosis54 05-01-2013 48 4   Download

Clostridial glutamate dehydrogenase mutants, designed to accommodate the 2¢-phosphate of disfavoured NADPH, showed the expected large speci-ficity shifts with NAD(P)H. Puzzlingly, similar assays with oxidized cofac-tors initially revealed little improvement with NADP + , although rates with NAD + were markedly diminished.

9p cosis54 05-01-2013 50 4   Download

This paper presents the experiences gained from ongoing research collaboration between the School of Computer Engineering at Nanyang Technological University and the Southampton e-Science centre at the University of Southampton using a serviceoriented approach for complex engineering design optimization. The service-oriented approach enables programmatic collaboration to be realized while maintaining the autonomy of individual codes at the different institutes and organizations. In the current work, a Genetic algorithm optimization logic implemented as a Grid...

6p khangoc239 10-08-2012 55 6   Download

EURASIP Journal on Applied Signal Processing 2003:3, 238–243 c 2003 Hindawi Publishing Corporation GA-Based Image Restoration by Isophote Constraint Optimization Jong Bae Kim Department of Computer Engineering, Kyungpook National University, 1370 Sangyuk-dong, Puk-gu, Daegu, 702-701, Korea Email: kjblove@ailab.knu.ac.kr Hang Joon Kim Department of Computer Engineering, Kyungpook National University, 1370 Sangyuk-dong, Puk-gu, Daegu, 702-701, Korea Email: kimhj@ailab.knu.ac.

6p sting12 10-03-2012 38 4   Download

Kỹ thuật gen hoặc công nghẹ gen (genetic engineering, genetic technology) là công nghẹ cốt lõi của công nghệ sinh học hiện đại được ra đời kể từ năm 1977. Công nghệ gen bao gồm các kỹ thuật thực hiện trên axit nucleic, nhằm nghiên cứu cấu trúc của gen, điều chỉnh và biến đổi gen, tách, tổng hợp và chuyển các gen mong muốn vào các tế bào sinh vật chủ mới tạo ra các cơ thể mới, sinh vật mới mang đặc tính mới. ...

17p thy_ht 22-02-2012 211 66   Download