Hydrochloride

Đề tài đã nghiên cứu tổng hợp được glibenclamide từ axit 5-chloro-2- methoxybenzoic với hiệu suất là 82,11%; đã đánh giá đƣợc hàm lượng sản phẩm glibenclamide theo tiêu chuẩn dược điển châu Âu với hiệu suất 99,95%,... Mời các bạn cùng tham khảo nội dung chi tiết.

50p closefriend04 17-10-2021 25 7   Download

Nội dung của Luận văn nghiên cứu sử dụng phương pháp sắc ký lỏng hiệu năng cao, phương pháp quang phổ hấp thụ phân tử dùng phổ toàn phần kết hợp với kỹ thuật tính toán và ứng dụng phần mềm máy tính đã được nghiên cứu và cho nhiều ưu điểm như độ nhạy, độ lặp, độ chính xác, độ tin cậy của phép phân tích cao, phân tích nhanh, tiện lợi. Mời các bạn cùng tham khảo!

79p fishbell 05-07-2021 23 5   Download

Nội dung Luận văn này nghiên cứu phân tích cấu trúc, hàm lượng của thuốc điều trị tiểu đường typ II metformin hydrochloride bằng các phương pháp hóa lý hiện đại là có ý nghĩa khoa học và thực tiễn. Mời các bạn cùng tham khảo!

64p fishbell 05-07-2021 32 3   Download

Pioglitazone hydrochloride (PGH) is an oral anti-hyperglycemic agent used in the treatment of type-2 diabetes mellitus. Potassium permanganate was found to oxidize PGH both in acidic and basic conditions, based on which two simple and sensitive methods were developed for its determination in bulk sample and tablets, and validated.

12p tocectocec 25-05-2020 14 2   Download

The current study presents the synthesis of thiophene-appended pyrazoles through 3+2 annulations of chalcones 3(a-g) with aryl hydrazine hydrochlorides 4(a-d) in acetic acid (30%) under reflux conditions produced the thiophene-pyrazole hybrids 5(a-g) in good yields.

8p tocectocec 25-05-2020 3 1   Download

Objectives of the dissertation: Research to improve the synthesis process of erlotinib hydrochloride drugs. Research on the synthesis and determination of quinazoline derivative structure. Research on the synthesis and determination of the structure of hybrid compounds of quinazoline derivatives and azides via triazole bridges.

26p xacxuoc4321 08-07-2019 32 5   Download

Protein refolding is currently a fundamental problem in biophysics and molecular biology. We have studied the refolding process of frutalin, a tetrameric lectin that presents structural homology with jacalin but shows a more marked biological activity. The initial state in our refolding puzzle was that proteins were unfolded after thermal denaturation or denaturation induced by guanidine hydrochloride, and under both conditions, frutalin was refolded.

6p research12 01-06-2013 43 4   Download

Unfolding, inactivation and dissociation of the lectin from Artocarpus hirsutaseedswere studiedbychemical (guanidine hydrochloride, GdnHCl) and thermal denaturation. Con-formational transitions were monitored by intrinsic ¯uor-escence and circular dichroism. The gradual red shift in the emissionmaxima of the native protein from335 to 356 nm, change intheellipticityat 218 nmandsimultaneousdecrease in the sugar binding activity were observed with increasing concentration of GdnHCl in the pH range between 4.0 and 9.0. ...

5p research12 29-04-2013 42 3   Download

Duckd2-crystallin is a soluble tetrameric lens protein. In the presence of guanidinium hydrochloride (GdnHCl),it undergoes stepwise dissociation and unfolding. Gel-filtra-tion chromatography and sedimentation velocity analysis has demonstrated the dissociation of the tetramer protein to a monomeric intermediate with a dissociation constant of 0.34lM 3 . Dimers were also detected during the dissociation and refoldingprocesses.

8p tumor12 20-04-2013 34 1   Download

Investigations of protein–solute interactions typically show that osmolytes favor native conformations. In this study, the effects of representative compatible and counteracting osmolytes on the reactivationof lactate dehydrogenase from two different conformational states were explored. Contrary to expectations, proline and trimethylamine oxide inhibited both the initial time course and the extent of reactivation of lactate dehydrogenase from bovine heart following dena-turation in guanidine hydrochloride, as well as following inactivation at pH 2.3.

12p fptmusic 12-04-2013 40 2   Download

To probe the stability of the seven-iron ferredoxin from Thermus thermophilus(FdTt), we investigated its chemical and thermal denaturationprocesses insolution.Aspredicted from the crystal structure, FdTt is extremely resistant to perturbation. The guanidine hydrochloride-induced unfolding transition shows a midpoint at 6.5M(pH 7, 20
C), andthe thermalmidpoint is aboveboiling, at 114
C. The stability of FdTt is much lower at acidic pH, suggesting that electrostatic interactions are important for the high stability at higher pH.

8p fptmusic 12-04-2013 58 5   Download

The CII protein of bacteriophagek, which activates the synthesis of thekrepressor, plays a key role in the lysis– lysogeny switch. CII has a small in vivo half-life due to its proteolytic susceptibility, and this instability is a key com-ponent for its regulatory role. The structural basis of this instability is not known. While studying guanidine hydro-chloride-assisted unfolding of CII, we found that low concentrations of the chaotrope (50–500 mM) have a con-siderable effect on the structure of this protein.

8p fptmusic 12-04-2013 37 2   Download

E2020 (R,S)-1-benzyl-4-[(5,6-dimethoxy-1-indanon)-2-yl]-methyl)piperidine hydrochloride isa piperidine-based ace-tylcholinesterase (AChE) inhibitor that was approved for the treatment of Alzheimer’s disease in the United States. Structure-activity studiesof thisclassof inhibitorshave indicated that both the benzoyl containing functionality and the N-benzylpiperidine moiety are the key featuresfor binding and inhibition of AChE.

12p fptmusic 12-04-2013 41 1   Download

The extreme thermal stabilization achieved by the introduction of a disul-fide bond (G8C⁄N60C) into the cysteine-free wild-type-like mutant (pWT) of the neutral protease fromBacillus stearothermophilus[Mansfeld J, Vri-end G, Dijkstra BW, Veltman OR, Van den Burg B, Venema G, Ulbrich-Hofmann R & Eijsink VG (1997)J Biol Chem 272, 11152–11156] was attributed to the fixation of the loop region 56–69.

12p awards 06-04-2013 43 3   Download

Thermodynamic parameters describing the conformational stability of the histidine-containing phosphocarrier protein fromStreptomyces coelicolor, scHPr, have been determined by steady-state fluorescence measurements of isothermal urea-denaturations, differential scanning calorimetry at different guanidinium hydrochloride concentrations and, independently, by far-UV circular dichroism measurements of isothermal urea-denaturations, and thermal denatura-tions at fixed urea concentrations.

17p dell39 03-04-2013 38 5   Download

The changes in the far-UV CD signal, intrinsic tryptophan fluorescence and bilirubin absorbance showed that the guanidine hydrochloride (GdnHCl)-induced unfolding of a multidomain protein, human serum albumin (HSA), followed a two-state process. However, using environment sensitive Nile red fluorescence, the unfolding and folding pathways ofHSA were found to followa three-state process andanintermediatewas detectedinthe range0.25–1.5M GdnHCl.

9p dell39 03-04-2013 40 4   Download

The functional activities of proteins are closely related to their molecular structure and understanding their structure–function relationships remains one of the intriguing problems of molecular biology. We investigated struc-tural changes in 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus(17b-HSDcl) induced by pH, temperature, salt, urea, guanidine hydrochloride, and coenzyme NADPH binding.

11p inspiron33 25-03-2013 41 4   Download

The unfolding pathways of the two forms ofPlasmodium falciparumacyl carrier protein, the apo and holo forms, were determined by guanidine hydrochloride-induced denaturation. Both the apo form and the holo form displayed a reversible two-state unfolding mechanism. The analysis of iso-thermal denaturation data provides values for the conformational stability of the two proteins.

14p galaxyss3 19-03-2013 29 4   Download

Onconase is an extremely stable member of the RNase A superfamily. The increase in the thermodynamic stability by 20 kJÆmol )1 in comparison to RNase A was expected to result in altered folding behavior. Despite the lack of cis-Pro residues in native Onconase, refolding at low concentrations of guanidine hydrochloride was complex and showed three kinetic phases (fast, medium, and slow), with rate constants differing by a factor of about 10 each.

8p media19 05-03-2013 36 3   Download

Poly(A)-specific ribonuclease (PARN) specifically catalyzes the degradation of the poly(A) tails of single-stranded mRNAs in a highly processive mode. PARN participates in diverse and important intracellular processes by act-ing as a regulator of mRNA stability and translational efficiency. In this article, the equilibrium unfolding of PARN was studied using both guani-dine hydrochloride and urea as chemical denaturants.

12p viettel02 22-02-2013 41 4   Download